Rapid and high-flux acute toxicity test method for luminous bacteria

Abstract

The invention relates to a rapid and high-flux acute toxicity test method for luminous bacteria, belonging to technical fields of analysis and detection. A luminous bacteria reviving solution is developed and can be used for rapidly reviving lyophilized luminous bacteria powder and cryopreserved strains within 30min, and the revived bacterium liquid can be directly used for a toxicity test as a working bacterium liquid without pre-culture after being stirred for a certain time. Meanwhile, a microporous plate is selected to serve as an exposing container and a detection tank for the toxicity test, a sample to be tested and the working bacterium liquid are added into pores and exposed for 15min, and then can be subjected to a luminous intensity test by adopting a chemiluminescence ELISA (Enzyme-Linked Immunosorbent Assay) reader. By adoption of the method, not only is a tedious and time-consuming working bacterium liquid pre-culture procedure omitted, but also the detection time is saved, the cost is reduced, the accuracy of a test result is increased, the high flux of the toxicity test is realized, and a wide popularization and application prospect is achieved.

Description

technical field [0001] The invention relates to a rapid and high-throughput acute toxicity test method for luminescent bacteria, which belongs to the technical category of environmental detection and evaluation. Background technique [0002] With the progress of society and the development of industrial and agricultural production, more and more chemicals are produced and used. At present, there are more than tens of millions of registered chemicals, and more than 100,000 chemicals in daily use. These chemicals may enter the environment through various channels during production and use, and then accumulate in the human body and organisms, posing a serious threat to the ecological environment and human health. In order to effectively manage chemicals and accurately evaluate the safety of environmental samples, biological toxicity testing has gradually developed into an essential and most intuitive and effective method. [0003] Luminescence bacterial toxicity detection is a...

AI Technical Summary

Problems solved by technology

[0005] However, the traditional method for testing the toxicity of luminescent bacteria has some deficiencies. First of all, the traditional methods recommend using freeze-dried powder to preserve the luminescent bacteria. The recovery process of the freeze-dried powder is generally time-consuming. Hours, slow ones need several days, so it takes more time to recover or pre-culture the strains, prolonging the time of toxicity test

Secondly, most of the traditional toxicity test methods use test tubes as exposure containers, and the amount of sample required for the test is large, and the amount of working bacteria solution is also large, which inevitably dilutes the concentration of the solution to be tested, resulting in inaccurate results

In addition, traditional method tests generally have a limited number of samples that can be tested each time, which is not conducive to the toxicity screening of a large number of samples

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