Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for real-time detection of the number of rare cells based on low-field nmr

A rare cell, real-time detection technology, applied in the field of molecular biology detection, can solve problems such as long centrifugation time, and achieve the effects of low price, simple and quick operation, and sensitive detection process.

Active Publication Date: 2017-02-08
上海美吉医学检验有限公司
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: ① longer centrifugation time; ② need to prepare inert gradient medium solution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for real-time detection of the number of rare cells based on low-field nmr
  • A method for real-time detection of the number of rare cells based on low-field nmr
  • A method for real-time detection of the number of rare cells based on low-field nmr

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Sample preparation

[0042] Preparation of samples to be tested

[0043] 1.1 Obtain an anticoagulated blood sample (~10 ml) from a breast cancer model mouse containing a mixed cell population suspected of containing circulating rare cells. Collected specimens should be processed the same day! Keeping time at room temperature away from light should not exceed 24 hours;

[0044] 1.2 Ficoll density gradient centrifugation method to obtain candidate mixed cell populations

[0045] 1) Add an appropriate amount of cell layering solution (prepared from Ficoll solution) to the bottom of sterile centrifuge tube A, then dilute the above anticoagulant test sample with PBS solution (2:1), and gently add On top of the layered liquid, the two form a clear interface.

[0046] 2) Centrifuge horizontally at 400×g for 30 minutes (the increase and decrease of the centrifuge speed should be uniform and stable to ensure the formation of a clear interface).

[0047] 3) After centrif...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting the number of rare cells in real time based on low field NMR (nuclear magnetic resonance). The method comprises the following steps: (1) preparing a standard sample and a to-be-tested sample of target rare cell suspension; (2) respectively adding an NMR contrast media coupled with a target rare cell specific expression antibody, mixing uniformly, and immediately and respectively carrying out NMR relaxation time detection before immune incubation; (3) carrying out constant-temperature immune incubation on the standard sample and the to-be-tested sample, and carrying out NMR relaxation time detection at same interval; (4) drawing an immune incubation time-relaxation time value standard curve, an immune incubation time-relaxation time variable standard curve and an incubation cell number-relaxation time variable maximum curve of the standard sample; and (5) computing the relaxation time variable of the to-be-tested sample, and measuring the concentration of rare cells in the to-be-tested sample according to the curves obtained in the step (4). The method can be used for detecting the concentration and the number of rare cells simply, conveniently, quickly, highly sensitively and directly in real time and with high specificity.

Description

technical field [0001] The invention relates to molecular biology detection technology, in particular to a detection method for rare cells in biological fluid samples. Background technique [0002] Rare cells refer to some atypical cells in biological fluid samples (including blood, pleural effusion, ascites, urine, cerebrospinal fluid, etc.). has important guiding significance. Therefore, finding an accurate and rapid detection method for rare cells will become an urgent problem to be solved. However, the concentration of rare cells in biological fluids is very low, with a ratio of about 1:10 to non-target cells 7 , cannot be counted with traditional techniques, so a simple, accurate and fast method is urgently needed to solve this difficult problem. [0003] Since the content of rare cells in body fluids is very small, effective enrichment steps are required to identify and identify them in subsequent experiments. At present, the methods widely used in the detection an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N24/08
Inventor 张祥林
Owner 上海美吉医学检验有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com