40results about How to "Shorten experimental operation time" patented technology

The invention discloses a preparation method of ultrathin slices aiming at a Hainan rubber tree leaf tissue. The preparation method comprises a preparation condition, a preparation device, preparationof various reagents and preparation of the ultrathin slices. A whole flow of the preparation method of the ultrathin slices aiming at the Hainan rubber tree leaf tissue comprises the following steps:firstly, infiltrating plant leaves in an immersion solution of glutaric dialdehyde; then fixing by utilizing osmic acid and dehydrating with ethanol; infiltrating by utilizing Epon 812 and embeddingand trimming blocks to prepare the ultrathin slices; dyeing by utilizing uranium acetate and lead citrate; finally, carrying out microscope inspection. By adopting the preparation method, the problemsof a traditional preparation method of ultrathin slices of plants including the grass family and the like for Hainan rubber tree leaves that a cell tissue structure is not clear in a transmission electron microscope sample preparation process, the slices are cracked when meeting water, an embedding agent is separated from the tissue, the embedding agent is easily affected with dampness and the like are solved; the experiment operation time is effectively shortened, and an effective technical method is provided for researching an ultra-microstructure of the rubber tree leaves.

The invention relates to an astrocyte separating and cultivating method, which is appropriate for various experimental animals and human. The method mainly aims at the problems in the prior art, and improves the method for separating and cultivating astrocyte in vitro at present. The method comprises five experimental steps: cerebral cortex tissue separation, filtering by a seive after tissue cell trituration, cell primary culture, continuous cell culture, and astrocyte identification. The whole method is simple and practicable. Purity of the astrocyte cultivated and obtained by the method reaches more than 95% and the astrocyte grows well, is rapid in cell proliferation, and can be continuously cultured for many times and meets the demand of various experiments of cytobiology.

The invention provides a human HLA-B27 gene typing kit. The human HLA-B27 gene typing kit comprises a PCR amplification primer, DNA polymerase and a reaction buffer solution, wherein the DNA polymerase is a hot initiated Taq polymerase which is modified by polyethylene glycol (PEG) and can tolerate PCR reaction inhibitors in blood. The invention also provides a nucleotide sequence of the PCR amplification primer. The human HLA-B27 gene typing kit has the technical effects that the adopted DNA polymerase is the hot initiated Taq polymerase which is modified by PEG and can tolerate PCR reaction inhibitors, PCR amplification on whole blood of human (nucleic acid extraction does not need to be carried out) can be realized, PCR amplification is directly carried out by virtue of blood, and DNA extraction does not need to be carried out; the human HLA-B27 gene typing kit can realize HLA-B27 negative and positive identification on a sample; meanwhile, a gene subtype result of an HLA-B27 positive sample can be obtained, and 24 HLA-B27 gene subtypes and other gene subtypes of 13 HLAB sites can be detected by every eight holes (eight linked pipes).

The invention relates to the field of sterile detection, in particular to a fully closed membrane filter for sterile examination of a sterile packaging interlayer and a detection method of the fully closed membrane filter. The fully closed membrane filter comprises two bacterium collecting incubators and an air filter device, wherein the two bacterium collecting incubators and the air filter device are connected to one end of a three-phase circulator by virtue of a conduit; the other end of the three-phase circulator is connected to a sterile sampling needle by virtue of a detection tube; and an intercepting clip is arranged on the part, close to the three-phase circulation valve (the three-phase circulator), of the conduit. By virtue of the fully closed membrane filter disclosed by the invention, secondary pollution on leaching liquor during operating is reduced, a microorganism detection rate is improved and an experimental result is better guaranteed. The circulation of medicine liquid in a sealed space is achieved and the medicine liquid is prevented from getting into contact with external side, so that the fully closed membrane filter plays a great role in the prevention of microbial pollution. Meanwhile, as for operation time, the experimental operation time is shortened by half, the detection efficiency of test article sterile examination is greatly improved, and a detection pollution rate is reduced to be less than 5% from original 20-25%.

The invention discloses a method for preparing a solid granular biochemical reagent, solving the problem that the conventional biochemical reagent cannot be accurately metered under the condition of zero gravity or bumping. The method mainly comprises the following steps of: (1) preparing the biochemical reagent; (2) distributing liquid drops through a liquid dispensing pump; (3) dropping the liquid drops into liquid nitrogen so that the liquid nitrogen in contact with the liquid drops absorbs heat so as to be gasified to form a gas flow, wherein the gas flow enables the liquid drops to be floated on the surface of the liquid nitrogen; and when the liquid drops are completely solidified, the liquid drops are formed into ice pellets and sunk to the bottom of the liquid nitrogen; and (4) freeze-drying the ice pellets in a freeze dryer, thereby obtaining the finished product. The method is high in accuracy, simple and convenient for operation, good in detection result and the like.

The invention discloses a chicken whole blood simple, convenient and quick DNA extraction method which comprises the following steps of after adding chicken whole blood in an anticoagulant, adding a mixture in lysate for mixing, putting a mixture in a water solution boiler with the temperature being 56-60 DEG C for 20-30min, adding a separating medium in a product, putting a mixture in the water solution boiler with the temperature being 70-80 DEG C for 20-30min for centrifugation, taking a supernate, transferring the supernate into another EP pipe, adding a precipitated solution, performing centrifugation, reserving precipitates, adding a washing solution, and performing uniform mixing and centrifugation; and carefully discarding the supernate, collecting the precipitates, drying the precipitates at the room temperature, and adding a TB buffer liquid to obtain a chicken DNA extracting solution. By adopting the chicken whole blood simple, convenient and quick DNA extraction method provided by the invention, the purity and the integrity of extracted DNA can both reach test requirements, and the concentration and the purity are superior to that of DNA extracted by an existing kit. Bycombining with application on a chicken molecular breeding aspect, proved that the method is an environment-friendly DNA extraction method with low cost and simple operation.

The invention relates to a method for directly converting plasma into hydrosulfite and application thereof. The method provided by the invention, in comparison with a similar hydrosulfite converted product on the market, has the greatest advantages that free DNA (Deoxyribonucleic Acid) of the plasma does not need to be extracted, the plasma can be directly used as a hydrosulfite conversion sample,and not only is the time of extracting the free DNA saved, but also the loss of the free DNA in an extraction process is avoided. Secondly, according to the method provided by the invention, in orderto protect the DNA from being influenced by miscellaneous protein, lipid and the like, a protective agent is added into a hydrosulfite conversion system. In addition, in order to further improve therecovery rate of the free DNA, the method adopts a high-sensitivity magnetic bead to carry out recovery. The entire conversion process does not exceed 2 hours; the centrifugal operation is not involved; the automation is more easily realized, and the method provided by the invention has the advantages of being low in cost, simple and convenient to operate, high in conversion ratio, high-purity inobtained DNA and the like.

The invention discloses a method for extracting genome DNA and RNA from a fine needle aspiration biopsy material. The method comprises the following steps: utilizing a turbine mixer to mix a cell lysis solution and a sample of a trace of biopsy material, implementing simultaneous extraction of DNA and RNA, and substituting the conventional liquid nitrogen grinding or mechanical pulverization method which can only extract the DNA and RNA respectively as the loss of the sample during the operation process is high. The method disclosed by the invention is simple in operation steps, low in cost, low in required sample capacity, short in experiment operating time, and capable of effectively preventing degradation of the DNA and RNA; the concentration, the purity and the integrity of the extracted DNA and RNA can meet the follow-up analytic requirement on genomics in various aspects.

The invention discloses a rat behavior infrared identification system, a using method and an application thereof. The system provided by the invention comprises a record display device, a treadmill, a signal collecting system, a plurality of infrared detectors and a device for reading rat EEG (Electroencephalogram), wherein a plurality of infrared detectors are respectively arranged on the rat behavior happened places; all of the infrared detectors and the device for reading rat EEG are connected with the signal collecting system, and the signal collecting system is connected with the record display device to record the rat special behavior with the rat EEG synchronously. The infrared detectors cooperate with the signal collecting system to detect the rotate speed of the treadmill, and the rat special movement behavior is also detected to be recorded as an event with the EEG synchronously, so as to analyze the change of EEG under the rat special movement behavior.

The invention relates to a fluorescence in-situ hybridization method for endosymbiotic bacteria in an insect ovary. The method comprises following steps: the insect ovary is dissected under a microscope, the dissected insect ovary is transferred to a glass slide, an insect tissue stationary liquid is added for fixation, and a destaining solution is added for destaining; pre-hybridization and hybridization staining are performed on a specimen, and an eluant is added for elution after hybridization is completed; finally, a sample is sealed with glycerin, and observation and photographing are performed under a confocal microscope. By means of the method, a good cell staining effect can be kept, the operation can be simplified, time can be shortened, reagents can be saved and the cost can be reduced. Several endosymbiotic bacteria in the ovary can be subjected to multiple staining simultaneously, a good staining effect is obtained, and the method is simple, convenient, rapid and economical.

The invention relates to a preparation method of a chromosome R banding. The method comprises the following steps: scribing a bone marrow cell suspension solution which is subjected to hypotension and is fixed, wherein an operation method of scribing comprises the following steps: holding a slide by a left hand to be inclined and holding a burette by a right hand to suck the cell suspension solution; slowly scribing along the upper edge of the slide from left to right; scribing and extruding the suspension solution so that the suspension solution uniformly flows to fully spread on a whole sheet; putting the slide into a constant-temperature box; controlling the temperature at 37 DEG C for 16 hours, and taking out the slide; and carrying out banding and dyeing. According to the method, a whole flow not only realizes standardization, but also has a stable effect; only a splitting phase quality item is compared; and by virtue of the method, the dispersion of chromosomes is not stopped by excessive interferences, the chromosomes are naturally dispersed until a fixed solution is completely volatilized, so that the dispersion degree is high. The ageing degree is sufficient and moderate so that the quality of a chromosome splitting phase is relatively good, and the banding pattern is relatively beautiful and clear and is easy to analyze.

The invention relates to a method for improving the fluorescence quantum yield of II-VI family quantum dots, and belongs to the technical field of nano materials and inorganic materials, and the method comprises the following steps: adding an anion source and a reducing agent into deionized water, and stirring and dissolving in a nitrogen atmosphere to form a transparent solution, thereby obtaining an anion precursor solution; dissolving a cation source in deionized water, adding a ligand, uniformly stirring, regulating the pH value to 7.5-10.5 by using an alkaline medium, and introducing nitrogen for half an hour to obtain a cation precursor solution. A CdTe quantum dot solution is subjected to post-treatment by adopting ZnCl2, and dangling bonds on the surface of the quantum dot are passivated by utilizing Zn < 2 + >, so that the surface defect of the quantum dot is passivated under the conditions that the band gap of the CdTe quantum dot is not influenced and the original light-emitting wavelength of the quantum dot is kept, and the fluorescence quantum yield is effectively improved; and the fluorescence quantum yield of the obtained product is greater than 30%.

The invention provides a multichannel small animal ultrasonic stimulation device. The multichannel small animal ultrasonic stimulation device is characterized by comprising a supporting mechanism, a motion control mechanism, a treatment head fixing frame, a multichannel ultrasonic stimulator and a plurality of ultrasonic treatment heads, wherein the supporting mechanism is provided with an animal experiment platform and a support arranged on the animal experiment platform; the motion control mechanism is provided with a plurality of three-dimensional motion units and a plurality of rotary motion units, and the three-dimensional motion units are arranged on the support; the treatment head fixing frame is arranged on the three-dimensional motion units and used for moving in the front-back direction, the up-down direction and the left-right direction under the driving of the three-dimensional motion units; the multichannel ultrasonic stimulator is fixed to the support and used for transmitting pulse signals; and the ultrasonic treatment heads are installed on the treatment head fixing frame through the rotary motion units respectively and used for rotating under the driving of the rotary motion units, each ultrasonic treatment head is provided with an ultrasonic transducer, and the ultrasonic transducers are in communication connection with the multichannel ultrasonic stimulator and used for converting the pulse signals into ultrasonic stimulation signals. The multichannel ultrasonic stimulator is connected with the ultrasonic transducers through a plurality of cables respectively, and therefore the pulse signals are transmitted.

The invention mainly discloses a method and device for testing the function of refining of fusion metal grains through ultrasonic waves. A single-chip microcomputer, an ultrasonic pulse generation receiver, ultrasonic probes and a heating device are included. The single-chip microcomputer and the ultrasonic pulse generation receiver are connected through a wire. The ultrasonic pulse generation receiver is connected with the ultrasonic probes through a multi-direction connector. An alloy is placed on the heating device, the single-chip microcomputer receives upper computer instructions, and signals are transmitted to the ultrasonic pulse generation receiver through the single-chip microcomputer. The ultrasonic pulse generation receiver generates negative-high-voltage pulse signals to drivethe multiple ultrasonic probes to send ultrasonic waves, and the ultrasonic waves refine the alloy liquefied after heating. After the ultrasonic probes stop working and the alloy is cooled and solidified, and the alloy refined through the ultrasonic waves is cut to be observed. The single-chip microcomputer is utilized for control, the multiple ultrasonic probes are controlled, and under the sameoutside conditions, the fusion metal grain refining functions are compared under different ultrasonic frequencies and ultrasonic action time periods.

The invention provides an adherent tumor cell cryopreservation method and an improved cryopreservation solution formula. By configuring an improved cell cryopreservation solution, the intermediate centrifugation step is omitted, so that the experimental operation time is greatly shortened, the experimental consumables can be saved, the experimental efficiency is increased, and the problems that the traditional adherent tumor cell cryopreservation method is longer in experimental operation time, needs to remove the culture solution by means of centrifugation, and is cumbersome in operation process and larger in damage to the cells, the activity of the resuscitated cells is lower, and the like can be solved. The adherent tumor cell cryopreservation method and the improved cryopreservation solution formula belong to the technical field of biology.

The invention discloses a DNA sulfite conversion and purification method. The method does need early sodium hydroxide denaturating treatment conducted on nucleic acid, adopts varying-temperature conversion conditions different from a commercialized reagent on the market and can perform a conversion experiment under the constant temperature condition. A magnetic bead method is adopted to perform nucleic acid purification after nucleic acid methylation conversion, a high-salt low pH value is utilized to perform nucleic acid separation and purification, then a low-salt high pH value is utilized to perform elution, the step of performing desulfonating by adopting sodium hydroxide on the market at present is omitted in the purification process, and meanwhile high-conversion-rate, high-quality and high-purity DNA can be obtained only by adopting a one-step washing step. The DNA sulfite conversion and purification method has the advantages of being quick, simple and convenient to operate and improving the conversion efficiency, the extraction efficiency and the extraction purity.

The invention provides a method for detecting biological activity of granulocyte colony stimulating factors. The method comprises the following three steps: constructing a cell line, preparing a standard substance and a test sample, and determining. The detection method is more convenient to operate, cells do not need to be specially treated, the operation time is shortened, and the experiment operation time is shortened by 1-2 days; meanwhile, according to the method, the detection flux is improved, the sample is diluted by 6-12 concentration points, the sample can be vertically arranged in a 96-well plate, and the sample detection number of each cell plate is increased.

The invention discloses a Chinese herbal medicine solid-phase extraction column. The Chinese herbal medicine solid-phase extraction column comprises a column tube and a packing in the column tube, wherein the packing is composed of the following components in parts by weight: 2-10 parts of carbon materials, 2-10 parts of amino-functionalized adsorbing materials and 1 part of silica gel material modified by a non-polar functional group; the carbon materials are fed in a bottom layer of the column tube; the amino-functionalized adsorbing materials and the silica gel material modified by the non-polar functional group are fed in an upper layer of the column tube in a mixing manner. The invention further discloses a sample pretreatment method for detecting pesticide residues in Chinese herbal medicines by using the Chinese herbal medicine solid-phase extraction column. The Chinese herbal medicine solid-phase extraction column is applied to sample pretreatment, can be used for analyzing and detecting pesticides and metabolic products in four Chinese herbal medicines namely ramulus mori, flos lonicerae, fructus lycii and folium nelumbinis, and has the characteristics that a sample is treated at one time; more than 400 pesticide residues in the Chinese herbal medicines are extracted and purified; the Chinese herbal medicine solid-phase extraction column is quick, economic and high in generality.

The invention discloses a method for testing chlorine resistance of an automobile part made of a polyamide material, and belongs to the technical field of performance testing of high polymer materials. The testing method comprises the steps that n cuboid splines are cut out from the surface of the automobile part made of the polyamide material, one end, in the length direction, of each spline is marked, the midpoint position of the tail end of each spline is marked as B, and the center, located on the same side of the midpoint of the tail end and having a certain distance from the midpoint of the tail end, of each spline is marked as A; one unmarked end of the sample strip in the step 1) is fixed on a clamping part of a clamp, the other marked end of the sample strip is positioned below a pulse pressure head, and the pulse pressure head is over against a point A of the sample strip; then, the influence of the chlorine salt on the mechanical property of the automobile part made of the polyamide material is judged by measuring the deformation quantity of the sample strip under different salt mist concentrations, different load acting forces and different times. The testing method designed by the invention not only is simple in sample preparation, but also is high in objective accuracy of an evaluation system.

The Invention discloses a preparation method of silage leach liquor, which is particularly practiced by mixing and immersing sterilized distilling water with silage; performing sonic oscillation afterlow-concentration hydrochloric acid treatment; filtering by a filter membrane, wherein the filter fluid is the silage leach liquor. Besides, the invention further provides an application of silage leach liquor prepared by the preparation method in the silage detection. Diluted hydrochloric acid with 1% of mass concentration is added during the preparation process of silage leach liquor; on the one hand, the low-concentration diluted hydrochloric acid can promote the complete dissolution of fermenting indexes in the silage such as organic acid and ammoniacal nitrogen; on the other hand, the viscosity between the silages is weakened after being treated by hydrochloric acid, the solid and fluid can be separated rapidly in the process of filtering by adopting the filter membrane, thereby greatly shortening the experimental operation time and saving labor force and lab consumables.

The invention relates to the field of sterile detection, in particular to a fully closed membrane filter for sterile examination of a sterile packaging interlayer and a detection method of the fully closed membrane filter. The fully closed membrane filter comprises two bacterium collecting incubators and an air filter device, wherein the two bacterium collecting incubators and the air filter device are connected to one end of a three-phase circulator by virtue of a conduit; the other end of the three-phase circulator is connected to a sterile sampling needle by virtue of a detection tube; and an intercepting clip is arranged on the part, close to the three-phase circulation valve (the three-phase circulator), of the conduit. By virtue of the fully closed membrane filter disclosed by the invention, secondary pollution on leaching liquor during operating is reduced, a microorganism detection rate is improved and an experimental result is better guaranteed. The circulation of medicine liquid in a sealed space is achieved and the medicine liquid is prevented from getting into contact with external side, so that the fully closed membrane filter plays a great role in the prevention of microbial pollution. Meanwhile, as for operation time, the experimental operation time is shortened by half, the detection efficiency of test article sterile examination is greatly improved, and a detection pollution rate is reduced to be less than 5% from original 20-25%.

The invention relates to the technical field of sugar content determination, and discloses a sucrose-glucose-fructose content determination kit and a determination method thereof. The sucrose-glucose-fructose content determination kit comprises a kit body, a reagent tube, a movable connecting device, a sealing cover and a locking device, wherein the reagent tube is fixedly arranged in the kit body; the movable connecting device is fixedly arranged on the right side of the kit body; and the sealing cover is fixedly arranged at the right end of the movable connecting device. According to the sucrose-glucose-fructose content determination kit and the determination method thereof, specific enzymes respectively act on sucrose, glucose and fructose, background interference of other substances ina sample is eliminated, and the detection result is accurate; and moreover, the three pieces of sugar data can be detected at one time, the experimental operation time is shortened, personal errors caused by batch detection are reduced, and a time-saving, money-saving and nontoxic detection means is provided for detection of a large number of samples.

The invention discloses a simple and quick DNA extraction method from chicken whole blood. The extraction method comprises adding anticoagulant to chicken whole blood, then adding it to lysate and mixing it, placing it in a water-soluble pot at 56-60°C for 20-30 minutes, and adding separation solution , put it in a water-soluble pot at 70-80°C for 20-30 minutes, then centrifuge, take the supernatant and transfer it to another EP tube, then add the eluate, centrifuge, leave the precipitate, add the washing solution, mix well, and centrifuge; carefully discard the supernatant , collect the precipitate and dry it at room temperature, add TB buffer to obtain the chicken DNA extract. The purity and integrity of the DNA extracted by the invention can meet the test requirements, and the concentration and purity are better than the DNA extracted by the existing kit. Combined with the application in chicken molecular breeding, it can be proved that the method is a low-cost, easy-to-operate and environment-friendly DNA extraction method.

The invention relates to rat vertebral plate bone scissors and a rat vertebral plate cut-out method adopting the same. The rat vertebral plate bone scissors comprise a handheld part and a cutting part. The handheld part comprises a left handheld handle, a right handheld handle, a screw for connecting the left handheld handle with the right handheld handle and an elastic sheet for connecting the tail of the left handheld handle with the tail of the right handheld handle. The cutting part comprises a tongue-shaped edge and an annular edge. The tongue-shaped edge and the left handheld handle are of an integrated structure. The annular edge and the right handheld handle are of an integrated structure. The two integrated structures are hinged through a screw. A certain included angle is formed by the front end of the tongue-shaped edge and the front end of the annular edge on the rat vertebral plate bone scissors. Blunt faces are arranged on the outer side of the tongue-shaped edge and the inner side of the annular edge, and the contact faces of the annular edge and the tongue-shaped edge are matched, so that the tongue-shaped edge and the annular edge are matched in the cutting process. According to the rat vertebral plate cut-out method adopting the rat vertebral plate bone scissors, the force, direction and force bearing point of cutting can be effectively controlled, locating is accurate, marrow is not prone to be infected, and the cutting process is fast and easy.

The invention discloses a rat behavior infrared recognition system and its use method and application, including a recording display, a treadmill, a signal acquisition system, several infrared detectors and a device for reading rat brain electrical signals, wherein, A number of infrared detectors were installed corresponding to the place where the rat’s behavior occurred; all infrared detectors and the device for reading the rat’s EEG signal were connected to the signal acquisition system, which was connected to the recording display, and the rat’s Specific behaviors of the rats were simultaneously recorded in the EEG signals of the rats. The infrared detector cooperates with the signal acquisition system to detect the speed of the treadmill and the specific movement behavior of the rat, and record it in the EEG signal synchronously in the form of an "event", which is used to analyze the EEG under the specific movement behavior of the rat. Signal changes.

The invention discloses a method for preparing a solid granular biochemical reagent, solving the problem that the conventional biochemical reagent cannot be accurately metered under the condition of zero gravity or bumping. The method mainly comprises the following steps of: (1) preparing the biochemical reagent; (2) distributing liquid drops through a liquid dispensing pump; (3) dropping the liquid drops into liquid nitrogen so that the liquid nitrogen in contact with the liquid drops absorbs heat so as to be gasified to form a gas flow, wherein the gas flow enables the liquid drops to be floated on the surface of the liquid nitrogen; and when the liquid drops are completely solidified, the liquid drops are formed into ice pellets and sunk to the bottom of the liquid nitrogen; and (4) freeze-drying the ice pellets in a freeze dryer, thereby obtaining the finished product. The method is high in accuracy, simple and convenient for operation, good in detection result and the like.