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A method for extracting genomic dna and rna from a fine needle aspiration material

A genome and fine-needle technology, which is applied in the field of preparation or purification of DNA or RNA, and separation, can solve the problems of less biopsy materials and cannot meet the needs of multi-level whole genome analysis, so as to achieve small sample size, reduce experimental operation time, and prevent Degradation effect

Active Publication Date: 2017-08-04
天津迈安诊生物技术有限公司 +1
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  • Abstract
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Problems solved by technology

[0005] The present invention provides a method for extracting genomic DNA and RNA from fine-needle aspiration biopsy materials in order to solve the problem that there are few biopsy materials and cannot meet the needs of multi-level genome-wide analysis.

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  • A method for extracting genomic dna and rna from a fine needle aspiration material
  • A method for extracting genomic dna and rna from a fine needle aspiration material
  • A method for extracting genomic dna and rna from a fine needle aspiration material

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Embodiment Construction

[0041] The present invention will be described in detail below in conjunction with the embodiments and the accompanying drawings.

[0042] 1. Main materials and equipment

[0043] The sample used in the present invention is a biopsy material obtained by a fine needle (7#) puncture.

[0044] TRIzol—Invitrogen Corporation.

[0045] The composition of the cell lysate was as follows: the final concentration of trishydroxymethylaminomethane (Tris) was 100 mmol / L, the final concentration of ethylenediaminetetraacetic acid (EDTA) was 500 mmol / L, and the final concentration of sodium chloride (NaCl) was 20 mmol / L, the final concentration of sodium dodecyl sulfate (SDS) is 10%, and the final concentration of trypsinase is 20ug / ml.

[0046] The composition of the TE solution was as follows: the final concentration of trishydroxymethylaminomethane (Tris) was 10 mmol / L, and the final concentration of ethylenediaminetetraacetic acid (EDTA) was 1 mmol / L.

[0047] The volume ratio of phen...

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Abstract

The invention discloses a method for extracting genome DNA and RNA from a fine-needle biopsy material. The present invention uses a vortex mixer to mix the cell lysate and the sample with a small amount of biopsy material, so as to realize the simultaneous extraction of DNA and RNA, which replaces the traditional liquid nitrogen grinding or mechanical pulverization method due to excessive sample loss during the operation. Only DNA and RNA can be extracted separately. The present invention has simple operation steps, low cost, less required sample size, short experimental operation time, and effectively prevents the degradation of DNA and RNA; the concentration, purity and integrity of the extracted DNA and RNA can meet the requirements of subsequent genomics. analysis requirements.

Description

technical field [0001] The invention relates to a method for separating, preparing or purifying DNA or RNA, in particular to a method for extracting genomic DNA and RNA from a fine needle puncture material, which can be used for downstream genomics analysis. Background technique [0002] According to the results of the third nationwide sample survey on the causes of death of residents conducted by the Ministry of Health and the Ministry of Science and Technology of my country, malignant tumors have become the second cause of death in my country, accounting for 22.32% of the total number of deaths, second only to cerebrovascular diseases. The city is the highest cause of death (accounting for 25.0% of the total number of urban deaths). In the past 30 years, the incidence of cancer has been increasing at an average annual rate of 3% to 5%. At present, the traditional imaging diagnosis and histocytology diagnosis used in medicine are to analyze the samples that have formed tumo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10
Inventor 王万恒陈晓瑞
Owner 天津迈安诊生物技术有限公司
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