Preparation method of chromosome R banding

Abstract

The invention relates to a preparation method of a chromosome R banding. The method comprises the following steps: scribing a bone marrow cell suspension solution which is subjected to hypotension and is fixed, wherein an operation method of scribing comprises the following steps: holding a slide by a left hand to be inclined and holding a burette by a right hand to suck the cell suspension solution; slowly scribing along the upper edge of the slide from left to right; scribing and extruding the suspension solution so that the suspension solution uniformly flows to fully spread on a whole sheet; putting the slide into a constant-temperature box; controlling the temperature at 37 DEG C for 16 hours, and taking out the slide; and carrying out banding and dyeing. According to the method, a whole flow not only realizes standardization, but also has a stable effect; only a splitting phase quality item is compared; and by virtue of the method, the dispersion of chromosomes is not stopped by excessive interferences, the chromosomes are naturally dispersed until a fixed solution is completely volatilized, so that the dispersion degree is high. The ageing degree is sufficient and moderate so that the quality of a chromosome splitting phase is relatively good, and the banding pattern is relatively beautiful and clear and is easy to analyze.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a preparation method for chromosome karyotype analysis R-banding in the field of medical examination. Background technique [0002] Chromosomal karyotype analysis is the process of pairing, numbering and grouping the chromosomes of the cells to be tested according to the inherent chromosomal morphological characteristics and regulations of the organism, and performing morphological analysis. Chromosomal karyotype analysis belongs to the category of morphology. Through manual analysis, macroscopic morphological abnormalities of chromosomes, such as deletions, increases or translocations, can be found to provide a basis for clinical diagnosis. According to different preparation processes, chromosome banding methods are divided into Q-banding, G-banding, R-banding, C-banding, T-banding, and N-banding, among which R-banding is the most commonly used, followed by G-banding. The mechanism...

AI Technical Summary

Problems solved by technology

The traditional method has three deficiencies. First, it is difficult to grasp the high-position drip sheet. It is impossible to drop the three positions of the head, body and tail very accurately. There may be overlapping parts, and even drips outside the sheet, causing waste and difficult to standardize.

Second, the degree of aging of the film is not fixed due to overheating, and the degree of overheating cannot be controlled. It is either excessively excessive or insufficient, and it is difficult to standardize

Third, only one piece can be dropped at a time in the high-position drop sheet and the overheating process, and two sheets are required for each specimen. In the case of a large number of specimens, it takes a long time

Fourth, if the film is left overnight at room temperature, it will be affected by the seasonal climate, causing the aging degree of chromosomes to be unstable, because the aging degree of chromosomes is inversely proportional to the banding time, and if the aging is not fixed, the banding time cannot be fixed, resulting in the quality of split images unstable

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