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Extraction method of genome DNA and RNA from fine needle aspiration material

A genome and fine-needle technology, which is applied in the field of separation, preparation or purification of DNA or RNA, can solve the problems of not being able to meet the needs of multi-level whole genome analysis and less biopsy materials, so as to reduce the experimental operation time, reduce the sample size and prevent Degradation effect

Active Publication Date: 2015-01-21
天津迈安诊生物技术有限公司 +1
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] The present invention provides a method for extracting genomic DNA and RNA from fine-needle aspiration biopsy materials in order to solve the problem that there are few biopsy materials and cannot meet the needs of multi-level genome-wide analysis.

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  • Extraction method of genome DNA and RNA from fine needle aspiration material
  • Extraction method of genome DNA and RNA from fine needle aspiration material
  • Extraction method of genome DNA and RNA from fine needle aspiration material

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Embodiment Construction

[0041] The present invention will be described in detail below in conjunction with the embodiments and the accompanying drawings.

[0042] 1. Main materials and equipment

[0043] The sample used in the present invention is a biopsy material obtained by a fine needle (7#) puncture.

[0044] TRIzol—Invitrogen Corporation.

[0045] The composition of the cell lysate was as follows: the final concentration of trishydroxymethylaminomethane (Tris) was 100 mmol / L, the final concentration of ethylenediaminetetraacetic acid (EDTA) was 500 mmol / L, and the final concentration of sodium chloride (NaCl) was 20 mmol / L, the final concentration of sodium dodecyl sulfate (SDS) is 10%, and the final concentration of trypsinase is 20ug / ml.

[0046] The composition of the TE solution was as follows: the final concentration of trishydroxymethylaminomethane (Tris) was 10 mmol / L, and the final concentration of ethylenediaminetetraacetic acid (EDTA) was 1 mmol / L.

[0047] The volume ratio of phen...

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Abstract

The invention discloses a method for extracting genome DNA and RNA from a fine needle aspiration biopsy material. The method comprises the following steps: utilizing a turbine mixer to mix a cell lysis solution and a sample of a trace of biopsy material, implementing simultaneous extraction of DNA and RNA, and substituting the conventional liquid nitrogen grinding or mechanical pulverization method which can only extract the DNA and RNA respectively as the loss of the sample during the operation process is high. The method disclosed by the invention is simple in operation steps, low in cost, low in required sample capacity, short in experiment operating time, and capable of effectively preventing degradation of the DNA and RNA; the concentration, the purity and the integrity of the extracted DNA and RNA can meet the follow-up analytic requirement on genomics in various aspects.

Description

technical field [0001] The invention relates to a method for separating, preparing or purifying DNA or RNA, in particular to a method for extracting genomic DNA and RNA from a fine needle puncture material, which can be used for downstream genomics analysis. Background technique [0002] According to the results of the third nationwide sample survey on the causes of death of residents conducted by the Ministry of Health and the Ministry of Science and Technology of my country, malignant tumors have become the second cause of death in my country, accounting for 22.32% of the total number of deaths, second only to cerebrovascular diseases. The city is the highest cause of death (accounting for 25.0% of the total number of urban deaths). In the past 30 years, the incidence of cancer has been increasing at an average annual rate of 3% to 5%. At present, the traditional imaging diagnosis and histocytology diagnosis used in medicine are to analyze the samples that have formed tumo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 王万恒陈晓瑞
Owner 天津迈安诊生物技术有限公司
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