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Kit for measuring content of total bile acid of blood serum and use method of kit

A serum total bile acid and kit technology, which is applied in the preparation of test samples, biological testing, measuring devices, etc., can solve the problems of unsatisfactory reagent stability and short reagent stability time, and achieve improved stability and improved stability. Accuracy, conversion-boosting effect

Inactive Publication Date: 2017-12-22
WHITMAN BIOTECH NANJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reagent provided in this invention has a short stable time when the lid is opened and used at 2-8°C; in the process of airtight and light-proof storage at 2-8°C, the stability of the reagent is not ideal. Prolonged, the reaction rate will decrease, and the color of the reagent will gradually change from colorless or light yellow to red

Method used

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  • Kit for measuring content of total bile acid of blood serum and use method of kit
  • Kit for measuring content of total bile acid of blood serum and use method of kit
  • Kit for measuring content of total bile acid of blood serum and use method of kit

Examples

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Effect test

Embodiment 1

[0042] The raw materials and proportions of the high-density lipoprotein cholesterol detection kit of this embodiment are as follows:

[0043] The kit consists of reagent 1 and reagent 2;

[0044] Reagent 1 is as follows:

[0045]

[0046] Reagent 2 is as follows:

[0047]

Embodiment 2

[0049] The raw materials and proportions of the high-density lipoprotein cholesterol detection kit of this embodiment are as follows:

[0050] The kit consists of reagent 1 and reagent 2;

[0051] Reagent 1 is as follows:

[0052]

[0053]

[0054] Reagent 2 is as follows:

[0055]

Embodiment 3

[0057] The raw materials and proportions of the high-density lipoprotein cholesterol detection kit of this embodiment are as follows:

[0058] The kit consists of reagent 1 and reagent 2;

[0059] Reagent 1 is as follows:

[0060]

[0061] Reagent 2 is as follows:

[0062]

[0063]

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Abstract

The invention discloses a kit for measuring content of total bile acid of blood serum and a use method of the kit, relates to the field of biochemical detection and aims to provide a high-stability, high-accuracy and non-interfering kit for measuring the content of total bile acid of blood serum and a use method of the kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises a GOOD'S buffer solution, thionicotinamide adenine dinucleotide, polyoxyethylene ether of nonylphenol, polyoxyethylene lauryl ether, ADP.2Na, EDTA (ethylene diamine tetraacetic acid), bovine serum albumin, mannitol and a preservative (proclin series); the reagent 2 comprises a GOOD'S buffer solution, 3 alpha-hydroxysteroid dehydrogenase, reduced coenzyme and the like. Due to addition of ethylene glycol, sucrose and multiple compound stabilizers, the stability of the reagents is improved; due to addition of EDTA, the interference effect of metal ions can be reduced and the test result accuracy can be improved.

Description

Technical field [0001] The present invention relates to the field of biochemical detection, in particular to a kit for measuring materials by measuring the chemical and physical properties of the materials. Background technique [0002] In 1974, the Norwegian Coming Company used purified 3α-hydroxysteroid dehydrogenase (3α-HSD) to successfully produce the first generation of bile acid enzymatic reagents, enabling the determination of bile acids to truly enter the clinical field. Since then, Norwegian Komin and Japan Daiichi Chemical have improved the reagent components of the first generation reagents, using starter reagents, and developed the second generation of bile acid determination reagents. Afterwards, Japan Daiichi Chemicals purified 3 from the genus Pseudomonas. -Oxy-5β-sterol-Δ4 dehydrogenase (Δ4DH) coupled with 3α-HSD to develop the third-generation bile acid determination kit. [0003] The Chinese patent CN1281983 application discloses a reagent for the determination o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/38G01N33/573G01N33/53
CPCG01N1/38G01N21/31G01N33/5308G01N33/5735G01N2333/902
Inventor 常俊骏孙文勇郑佳
Owner WHITMAN BIOTECH NANJING
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