Kit for measuring content of total bile acid of blood serum and use method of kit

Abstract

The invention discloses a kit for measuring content of total bile acid of blood serum and a use method of the kit, relates to the field of biochemical detection and aims to provide a high-stability, high-accuracy and non-interfering kit for measuring the content of total bile acid of blood serum and a use method of the kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises a GOOD'S buffer solution, thionicotinamide adenine dinucleotide, polyoxyethylene ether of nonylphenol, polyoxyethylene lauryl ether, ADP.2Na, EDTA (ethylene diamine tetraacetic acid), bovine serum albumin, mannitol and a preservative (proclin series); the reagent 2 comprises a GOOD'S buffer solution, 3 alpha-hydroxysteroid dehydrogenase, reduced coenzyme and the like. Due to addition of ethylene glycol, sucrose and multiple compound stabilizers, the stability of the reagents is improved; due to addition of EDTA, the interference effect of metal ions can be reduced and the test result accuracy can be improved.

Description

Technical field [0001] The present invention relates to the field of biochemical detection, in particular to a kit for measuring materials by measuring the chemical and physical properties of the materials. Background technique [0002] In 1974, the Norwegian Coming Company used purified 3α-hydroxysteroid dehydrogenase (3α-HSD) to successfully produce the first generation of bile acid enzymatic reagents, enabling the determination of bile acids to truly enter the clinical field. Since then, Norwegian Komin and Japan Daiichi Chemical have improved the reagent components of the first generation reagents, using starter reagents, and developed the second generation of bile acid determination reagents. Afterwards, Japan Daiichi Chemicals purified 3 from the genus Pseudomonas. -Oxy-5β-sterol-Δ4 dehydrogenase (Δ4DH) coupled with 3α-HSD to develop the third-generation bile acid determination kit. [0003] The Chinese patent CN1281983 application discloses a reagent for the determination o...

AI Technical Summary

Problems solved by technology

The reagent provided in this invention has a short stable time when the lid is opened and used at 2-8°C; in the process of airtight and light-proof storage at 2-8°C, the stability of the reagent is not ideal. Prolonged, the reaction rate will decrease, and the color of the reagent will gradually change from colorless or light yellow to red

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