Stable reagent for determining alanine aminotransferase

A technology of alanine amino and medium alanine amino, which is applied in the fields of biochemistry and clinical testing, and can solve problems such as reagent instability, difficulty in detecting serum ALT, and accelerated stability experiments

Active Publication Date: 2018-01-16
BEIJING BEIJIAN XINCHUANGYUAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process also cannot avoid changes in temperature and humidity, and the sample is exposed to air after opening the cover, facing oxidation factors
Therefore, in this case, the accelerated stability test cannot evaluate the stability of other aspects (ISO standard 23640-2011, In vitro diagnostic medical devices-Evaluation of stability of in vitro diagnostic reagents)
[0008] At present, the double-reagent enzyme-coupled continuous detection method is mostly used in the market to detect serum ALT. Due to the presence of enzyme and NADH in the reagent, the reagent is unstable during transportation and when the bottle cap is opened for operation on the machine. ALT brings difficulty

Method used

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  • Stable reagent for determining alanine aminotransferase
  • Stable reagent for determining alanine aminotransferase
  • Stable reagent for determining alanine aminotransferase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1: the preparation of comparison kit

[0064] First Reagent R1:

[0065]

[0066] Second reagent R2:

[0067] TRIS, pH6.5 100mmol / L

[0068] L-alanine 1500mmol / L

[0069] α-ketoglutarate 45mmol / L

Embodiment 2

[0070] Embodiment 2: the preparation of test kit

[0071] The preparation method of the second reagent R2 is the same as in Example 1; the preparation method of the first reagent R1 is shown in Table 1.

[0072] Table 1. R1 reagent composition of different test kits

[0073]

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PUM

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Abstract

The invention provides a stable reagent for determining alanine aminotransferase. The reagent is a dual reagent including TRIS, NADH, LDH, EDTA, TritinX-100, BSA, and Proclin. Through the reagent, thethermal stability, airboard stability and transportation stability of an in vitro diagnostic reagent of an NADH indicating system can be effectively improved, and the storage time of a kit can be effectively prolonged.

Description

technical field [0001] This application relates to the fields of biochemistry and clinical testing. More specifically, the present application relates to a reagent for assaying alanine aminotransferase. Background technique [0002] Alanine aminotransferase (ALT) mainly exists in blood and related tissues. The intracellular concentration is 1000 to 3000 times higher than the serum concentration. As long as 1% of the liver tissue is destroyed, the serum concentration can be doubled. It is recommended by the World Health Organization as the most sensitive detection index of liver function damage. Determination of ALT activity on toxic hepatitis, viral hepatitis, active liver cirrhosis, liver cancer, obstructive jaundice, fatty liver, cholangitis and cholecystitis, myocardial infarction, myocarditis, cardiovascular disease, skeletal muscle disease, internal organs and muscles The diagnosis of inflammatory necrosis etc. has important value ("Clinical Liver Disease Experimental...

Claims

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Application Information

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IPC IPC(8): G01N21/75
CPCY02A50/30
Inventor 姜敏
Owner BEIJING BEIJIAN XINCHUANGYUAN BIOLOGICAL TECH
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