Buffer solution for human serum alanine aminotransferase measurement

Abstract

The invention discloses a buffer solution for human serum alanine aminotransferase measurement, is used in a kit by using Tris-citric acid as a buffer system. The kit comprises a first reagent and a second reagent, wherein the first reagent is composed of Tris, alanine, NADH (nicotinamide adenine dinucleotide), lactate dehydrogenase, sodium azide and citric acid; and the second reagent is composed of Tris, alpha-ketoglutarate, sodium azide and citric acid. The buffer solution for human serum alanine aminotransferase measurement adopts the Tris-citric acid as the buffer system. The buffer solution can provide benign conditions for enzymatic reaction of alanine aminotransferase in human serum; and the matched kit has higher accuracy and stability of measurement data than the existing reported buffer solutions, and thus, is worthy of popularization and application.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a buffer solution for measuring human serum alanine aminotransferase. Background technique [0002] Alanine aminotransferase (ALT), commonly known as alanine aminotransferase, is an enzyme involved in human protein metabolism (equivalent to a catalyst in industrial production), and plays a role in accelerating the conversion of protein amino acids in vivo. The concentration is the highest in the cytoplasm of the human body, and the content is less in tissues and organs such as kidney, heart, and skeletal muscle. Elevated alanine aminotransferase in the human body is usually caused by certain liver-related diseases such as liver cirrhosis, liver cancer, viral hepatitis, etc., and is also common in extensively damaged muscle diseases or circulatory failure with shock, hypoxia, myocardial Infarction and hemolytic disease. Therefore, in clinical medicine, alanine aminotransferase ...

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Problems solved by technology

[0005] The above reaction causes NADH to decrease, causing the absorbance to decrease at a wavelength of 340nm, and the rate of decrease is proportional to the activity of alanine aminotransferase (ALT)

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