The invention relates to a method for screening high-yield
amphotericin B streptomyces nodosus through high-
throughput mutagenesis, a screened
mutant strain, a recombinant
engineering bacterium obtained from the
mutant strain and an application thereof. The
streptomyces nodosum ZJB2016050 which is a
streptomyces nodosum strain capable of highly yielding
amphotericin B is obtained by screening in the invention. The method disclosed by the invention has the beneficial effects that 1, the yield of
amphotericin B can be conveniently, quickly and efficiently detected by a high-
throughput amphotericin B high-yield strain
screening method, and the
mutation breeding and screening or
verification efficiency of
genetic engineering strains can be improved; 2, compared with an original strain N3, thestreptomyces nodosus N5 obtained by
mutation breeding has the advantages that the amphotericin B yield is increased by 20%, and the streptomyces nodosus N5 can be used as the original strain of
genetically engineered bacteria; 3, the yield of amphotericin B is respectively increased by overexpression of
functional genes, wherein the yield of amphotericin B is increased by 20% by
acetyl coenzyme Acarboxylase 1 (acc1), and 4, the
kanamycin is low in price, wide in antibacterial spectrum and strong in
bactericidal effect, is suitable for industrial use, has a relatively strong effect of improving the total yield of enterprises, and can reduce the cost by about 2000 yuan per tank by taking a 5L tank in a laboratory as a calculation result.