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Drug for inhibiting enterovirus 71

An enterovirus and drug technology, applied in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., to achieve the effect of good market value and clinical application prospects

Active Publication Date: 2015-05-13
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] With the in-depth study of EV71 molecular biology, many antiviral drugs have been designed for different targets in the process of EV71 replication, but most of their antiviral effects and adverse reactions in vivo need further in vivo experiments and clinical verification.

Method used

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  • Drug for inhibiting enterovirus 71
  • Drug for inhibiting enterovirus 71
  • Drug for inhibiting enterovirus 71

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Drug Inhibition Experiment on EV71

[0030] 1. Method RD cells (8×10 5 ) or 293 cells (6×10 5 ) in six-well plates without antibiotics in complete DMEM medium, cultivated overnight, and when the cell growth was more than 90%, RD cells were treated with 0, 1, 2, 3, 4, 5 μM amphotericin B, and after 2 hours Add MOI: 0.08 of EV71 to infect respectively. 293 cells were treated with amphotericin B at 0, 0.2, 0.5, 1, 2, and 3 μM, and then infected with EV71 at an MOI of 0.5 after 2 hours. After incubating in the incubator for 1 hour, replace it with normal medium and culture for the corresponding time, collect the supernatant, and carry out the virus plaque formation test. At the same time, the cells were collected, and Western Blotting experiments were performed after lysing to detect the expression of viral proteins in the cells.

[0031] 1.1 Virus plaque formation experiment

[0032] RD cells (3×10 5 ) inoculated in a 12-well plate, and when the cells were ...

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Abstract

The invention provides a drug for inhibiting enterovirus 71. Amphotericin B for clinically treating fungal infection is found to have the characteristic of inhibiting enterovirus 71; through a drug toxicity test, an inhibition test of the drug to viruses and a virus plaque formation test, the amphotericin B is found to obviously inhibit infection of the EV71 virus in an RD cell and a 293 cell; furthermore, the amphotericin B is found to play the virus infection resistance through influencing the adsorption of the EV71 virus. The drug provided by the invention is a novel drug for preventing and treating the enterovirus 71, and is higher in market value and good in clinical application prospect.

Description

technical field [0001] The present invention relates to the field of antiviral drugs, in particular to a drug for inhibiting enterovirus 71. Background technique [0002] Hand, foot and mouth disease has broken out and spread in many parts of the world, especially in Asia, and its infection rate and death rate are increasing year by year, and the harm is very serious. Enterovirus 71 (EV71) is the main pathogen of hand, foot, and mouth disease (HFMD), mainly infecting infants, and its infection is often accompanied by neurological complications, which can lead to death in children . At present, although there are some reports on antiviral drugs targeting EV71 replication cycle, vaccine development of EV71, RNA, etc., so far, no effective preventive measures and treatments have been found. [0003] In order to find an effective treatment for EV71 infection, scholars at home and abroad have achieved some research results in drug development. One is to use existing antiviral ...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P31/14
Inventor 郭斐
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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