Preserving fluid for preserving virus samples for long time at normal temperature and application thereof

Abstract

The invention provides a preserving fluid for preserving a virus sample at normal temperature for a long time. The preserving fluid is equipped whit nutrient substances such as salts, amino acids, glucose and the like required by virus survival, is added with virus protective agents such as polyvinylpyrrolidone, mannitol, trehalose and the like for protecting viruses, and is added with bacteriostatic agents such as amphotericin B, vancomycin and the like for inhibiting the growth of bacteria. The preserving fluid is safe and non-toxic, is simple to use, does not need cold chain preservation, can be used for transporting and preserving the virus sample at normal temperature for 3 days, and effectively solves the problems that the virus samples depend on cold chains in the sampling, transporting and preserving processes and the preservation time is short. The preserving fluid can maintain the primitiveness of the virus sample, is beneficial to comprehensive analysis and research of the virus sample, and is suitable for collection and transportation of epidemic virus samples.

Description

technical field [0001] The invention relates to the field of biomedical technology, in particular to a preservation solution for preserving virus samples at room temperature for a long time and an application thereof. Background technique [0002] A virus is a special organism without a cellular structure, consisting of a protein shell and genetic material inside. Viruses are tiny and simple in structure, containing only one kind of genetic material (DNA or RNA). Viruses have no metabolic mechanism, no enzyme system, and cannot survive independently. They can only parasitize in host cells, rely on the materials and energy in the host cells to complete life activities, and reproduce, transcribe, and translate according to the genetic information contained in them to produce a new generation Viruses reproduce by replication. After replication, the new generation of virus can lyse the original host cell and infect the new host cell for the next round of replication. Viral in...

AI Technical Summary

Problems solved by technology

However, affected by transportation, cold chain, storage and other factors, the storage time of Hanks solution is short, and the integrity of virus morphology is easily affected, which is not conducive to the isolation and identification of viruses, and is also not conducive to the extraction rate of viruses, and it is easy to breed bacteria and fungi. This leads to a drop in the pH value of the Hanks solution. When the pH value of the Hanks solution is less than 7.4, the stability of the virus decreases, the decomposition speed of the virus is accelerated, and it is difficult to preserve the virus activity for a long time.

The existing virus sample collection and storage methods rely on cold chain transportation, and based on the consideration of virus activity, the storage time is mostly within 48 hours, which not only increases the cost of sample transportation and storage, but also limits the distance between sample collection points and diagnostic experiments. The possibility and distance between them directly affect the accuracy of the experimental detection results

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