Abalone cell culture medium and abalone cell culture method

Abstract

The invention relates to an abalone cell culture medium and an abalone cell culture method. The abalone cell culture medium comprises an L-15 basal culture medium and mixed serum, wherein the mixed serum comprises fetal calf serum accounting for 15% of the volume of the abalone cell culture medium and inactivated abalone serum accounting for 5% of the volume of the abalone cell culture medium; thebasal culture medium comprises the following components: 100 * of a penicillin streptomycin mixed solution, gentamycin, amphotericin B, NaCl2, KCl, CaCl2, MgSO4 and MgCl2. According to the invention,polylysine coating improvement is performed on the surface of a six-hole cell culture plate, so that a complete culture medium formula for primary culture of haliotis discus hannai Ino blood cells isadjusted, and the haliotis discus hannai Ino blood cells having strong adherence capacity, stable state and good activity can be rapidly obtained in a short time. In addition, the abalone cell culture medium can also provide abalone cells for studies on nutrition, immunity, environmental toxicity, gene function analysis and the like.

Description

technical field [0001] The invention belongs to the technical field of animal cell culture, and in particular relates to a culture medium for abalone cells and a method for culturing abalone cells. Background technique [0002] Haliotis discus hannai Ino is one of the important aquaculture shellfish in my country. Abalone aquaculture has developed rapidly in recent years, but at the same time, it is also facing illness and death caused by high temperature in summer and pathogens. In terms of research on the nutritional requirements of wrinkled abalone, the research on the requirements of amino acids, fatty acids and other nutrients and physiological metabolism needs to be further studied. [0003] The slow growth rate and long breeding period of abalone limit the exploration efficiency of in vivo experiments, and in vivo experiments are affected by many environmental factors, which cannot accurately reflect the deep molecular mechanism. Therefore, in addition to the traditi...

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Problems solved by technology

[0005] In view of this, the purpose of the present invention is to overcome the deficiencies of the prior art and provide a culture medium for abalone cells and a method for culturing abalone cells to solve the problem that the abalone blood cells cultured in the prior art are difficult to meet the experimental requirements

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