Culture medium for helicobacter pylori as well as preparation method and application of culture medium

A Helicobacter pylori, Helicobacter pylori technology, applied in the field of microbiology, can solve the problems of no advantages, inability to adapt to the requirements of Helicobacter pylori isolation and culture, and low positive detection rate

Pending Publication Date: 2022-02-15
SUZHOU GUOKE MEDICAL TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional isolation medium cannot meet the requirements for the isolation and cultivation of Helicobacter pylori. Commonly used media such as...

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  • Culture medium for helicobacter pylori as well as preparation method and application of culture medium
  • Culture medium for helicobacter pylori as well as preparation method and application of culture medium

Examples

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Embodiment 1

[0040] Embodiment 1 This embodiment provides a medium for pylorus spiral bacilli and a preparation method thereof

[0041] 1.1 Medium Formulation:

[0042] Agar 5g, cysteine ​​8g, antibiotic 0.5 mg, soluble starch 6g, 0.1 ml of lactic acid and horses 8g;

[0043] In the antibiotics, the mass fraction of vancomycin, amphotericin B and polymyxin is: vancomycin: 49%; amphotericin B: 49%; polymyxin: 2%.

[0044] 1.2 Reagent material source:

[0045] Joan: Beijing Luqiao Technology Co., Ltd.; cysteine, soluble starch: Annegi Chemical Co., Ltd .; 万, polyphenycin, amphotericin B: BIOSHARP; lactic acid: National Pharmaceutical Group; Ma Quan Blood: Jilin University Medical College Animal Center.

[0046] 1.3 Preparation method:

[0047] S1: Take 0.245 mg according to the formulation, amphotericin B 0.245 mg and polymyxin 0.01 mg dissolved in an appropriate amount of sterile water, mix well, irradiated under ultraviolet light, spare;

[0048] S2: According to the formulation quantity, solu...

Embodiment 2

[0050] Example 2 This example provides a medium of Helicobacterius and a preparation method thereof

[0051] 1.1 Medium Formulation:

[0052] Agar 5g, cysteine ​​8g, antibiotic 0.5 mg, soluble starch 6g, 0.1 ml of lactic acid, 3 mg of pylori rod bacteria lipopolysaccharide extract 3mg and horses 8g;

[0053]In the antibiotics, the mass fraction of vancomycin, amphotericin B and polymyxin is: vancomycin: 49%; amphotericin B: 49%; polymyxin: 2%.

[0054] 1.2 Preparation of Helicobacter bacteria lipopolysaccharide extract

[0055] First activation, screening, expanding cultivating, screening, and expanding cultivating, and collecting a bacterial suspension is collected in a bacterial suspension. The bacterial suspension was centrifuged by 5000 rpm for 10 min to precipitate. After washing with PBS buffer, 5000 rpm was centrifuged for 10 min, repeated 2 times. The precipitate (bacteria) was resuspended with PBS buffer, and the concentration of the bacteria was 2.0 × 10 10 A / ml, made ...

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Abstract

The invention discloses a culture medium for helicobacter pylori as well as a preparation method and application of the culture medium, and belongs to the technical field of microorganisms. Specifically, the invention provides the culture medium for the helicobacter pylori, and every 1000 mL of the culture medium for the helicobacter pylori contains 3 to 10 g of agar, 5 to 10 g of cysteine, 0.3 to 0.8 mg of antibiotics, 3 to 10 g of soluble starch, 0.1 to 1 mL of lactic acid and 1 to 10 g of horse whole blood. Wherein the antibiotics are prepared from vancomycin, amphotericin B and polymyxin; in the antibiotics, the mass fraction of vancomycin is 30-60%, the mass fraction of amphotericin B is 30-60%, and the mass fraction of polymyxin is 30-60%. 30% to 80% of amphotericin B; and 0.1%-10% of polymyxin. The invention also provides a preparation method and application of the culture medium. The culture medium provided by the invention is suitable for quick growth and culture of helicobacter pylori, can effectively inhibit the growth of infectious microbes, reduces the use of antibiotics, and has great use value.

Description

Technical field [0001] The present invention belongs to the field of microbial technology. In particular, the present invention relates to a medium for the medium for pylorial spiral bacilli and a preparation method thereof. Background technique [0002] Helicobacter pylori (HP) is a Gram-negative spiral bacterium that is planted between gastric mucosal epithelium and mucus, which shows that it is closely related to chronic gastritis, digestive ulcer, lymphatic hyperplasia. Relevant, is important in pathogens. Since the first separation in 1983, the technology of testing the spiral bacillus of the testor has a large progress, mainly divided into two major categories, one is invasive detection technology, including live tissue sections, fast urea enzyme, bacterial culture Method and PCR method; the other is non-invasive detection technology, including 13 C or 14 C-urease exhalation test, serum immunological test, and feces antigen detection, etc. The bacterial culture method is th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C12R1/01
CPCC12N1/20C12N1/38
Inventor 李勇段生宝于海利王红梅陈晔洲丁少华田晶晶王玉珏刘杰王泽龙王晴晴
Owner SUZHOU GUOKE MEDICAL TECH DEV CO LTD
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