174 results about "Antibiogram" patented technology

The invention provides a high-efficiency compound microbial inoculum for fermenting beds, particularly relates to a compound microbial inoculum for the fermenting beds, and a preparation method and application thereof. The compound microbial inoculum is composed of five beneficial bacterium strains (Bacillus subtilis CGMCC1.769 and CGMCC1.210, lactobacillus plantarum CICC6073, Candida tropicalis CGMCC2.587 and Trichoderma koningii CGMCC3.4290) and metabolites thereof (amylase, protease, lipase, cellulase, organic acid, bacteriophage and the like). The compound microbial inoculum is prepared by using a submerged liquid mixed fermentation technique. The method comprises the following steps: carrying out liquid submerged fermentation on the lactobacillus plantarum at 35 DEG C for 12 hours, respectively inoculating Candida tropicalis and Trichoderma koningii, carrying out mixed fermentation at 30 DEG C for 12 hours, inoculating Bacillus subtilis, carrying out liquid submerged mixed fermentation for 24 hours, respectively controlling the fermented viable count at 1-2*10<9> cfu / mL, 5-8*10<8> cfu / mL, 3-5*10<8> cfu / mL, 3-5*10<9> cfu / mL and 2-5*10<9> cfu / mL, finishing fermentation, packaging and sealing to obtain the high-efficiency compound microbial inoculum for the fermenting beds. The invention also provides application of the high-efficiency compound microbial inoculum-a preparation method of padding material. The compound microbial inoculum has the advantages of high viable count, high biological activity and low impurity bacterium pollution; the compound microbial inoculum can obviously reduce the odor of pigsties and reduce the living possibility of mosquitoes and flies, thereby achieving the ecological culture goals of no pollution and zero discharge; and the process for manufacturing the ferment bed is simple, thereby saving the padding material, human power and the like.

Use of a macrolide antibiotic for the manufacture of a medicament for the treatment or prevention of a cancer selected from colorectal cancer, Desmoid tumor, bladder cancer, gastric cancer, and breast cancer, the macrolide antibiotic being one or more of : tylosin, pharmaceutically acceptable salts thereof, and derivatives thereof; erythromycin, pharmaceutically acceptable salts thereof, and derivatives thereof; oleandomycin, pharmaceutically acceptable salts thereof, and derivatives thereof; and spiramycin, pharmaceutically acceptable salts thereof, and derivatives thereof. Also provided are pharmaceutical compositions and methods for the treatment or prevention of the above mentioned cancers and methods for treating or preventing, in a mammal, a cancer that expresses a mutated APC gene.

The invention relates to Mulin acetate containing substituted squaric acid and an application thereof which belong to the technical field of medicines, specifically to substituted squaric acid containing Mulin acetate as shown in a general formula (I), pharmaceutically acceptable salt, a hydrate and an isomer thereof, wherein R1, R2, R3, R4, R5 and B are defined in the specification. The invention also relates to a preparation method of these compounds, a pharmaceutical composition containing these compounds and an application of these compounds in the preparation of drugs for treating or preventing bacteria and virus. As for staphylococcus aureus and Streptococcus equin MIC value, the compounds have an antibacterial effect 15-20 times higher than a control commercially-available antibiotic tiamulin in the test. And the compounds provided by the invention are effective antimicrobial agents. And the compounds provided by the invention are effective antimicrobial agents.

The invention discloses a midget anti drug-tolerance staphylococcus aureus polypeptide comprised of passage structure domain to form ion channel colicine and staphylococcus aureu pheromone, as well as opposite coded nucleotide sequence and recombination plasmid contained said sequence. Wherein, the plupeptide can not induce bacteria to generate trsditional drug-tolerance by the mechnism that it forms directly ion channel on cell film of target cell to kill bacteria. This invention has more single antimicrobial spectrum without effect to Gram-negative bacteria of escherichia coli, shows at least 500 times effect to pheneazonecillin, and can be used as synergist to beta-lactam antibiotics.

Belonging to the technical field of Chinese medicine preparation methods, the invention relates to a preparation method of a Chinese medicinal lotion treating dark urine-type cellulitis. Current treatment of dark urine-type cellulitis generally employs antibiotics and sulfonamides, and has the disadvantage of great side effect. A technical scheme adopted by the invention consists of: taking Polyporus, Rhizoma Dioscoreae Hypoglaueae, coix seeds, waxgourd peels, Phaseolus calcaratus, Zanthoxylum bungeanum Maxim, Corn Stigma, gourds, Commelina communis, Polygonum aviculare, Dianthus superbus, akebia stems, Ricepaperplant Pith, Juncus effusus, Patience Dock Roots, Ilex pubescens, Herba Patriniae, Changium smyrnioides, Hemsleya macrosperma, Anemarrhena asphodeloides, radix tinosporae, Trollius chinensis, honeysuckle, Bidens biternata, houttuynia cordata, Herba Lespedezae Cuneatae, hypericum perforatum, Sedum sarmentosum, Sagina subulata, convallaria majalis and licorice, and placing the 31 medicines into water for immersion, then conducting decocting with slow fire, and carrying out filtration to remove the residue, thus obtaining a liquid medicine, i.e. the Chinese medicinal lotion treating dark urine-type cellulitis. The prepared Chinese medicine has the advantages of simple preparation, small toxic and side effect, short treatment course, and high curative rate.

The invention discloses a diastole bar apron sodium antibiotics and preparing craft, which is characterized by the following: allocating with mass ratio as 100-400 antibiotics, 100-400 diastole bar apron sodium, 10-300 liposome and 10-300 polyvinyl pyrrolidon; keeping the drug-effect of common diastole bar apron sodium antibiotics compound; prolonging nullifying half-decay time of the antibiotics; adding antioxidant into composite drug; increasing stability of the drug. This invention can prolongs the effective time of the drug.

The invention discloses an orthopaedic metal implant loaded with slow release antibiotic, composed of a matrix (1) and slow release antibiotic layer (2) covered on the matrix; wherein the matrix (1) is titanium alloy, or stainless steel or cobalt molybdenum alloy, and the slow release antibiotic layer (2) is a PDLLA coating combined with antibiotic. The preparation method includes pretreatment ofthe matrix (1), confection of antibiotic solution and sedimentation of the coating. The orthopaedic metal implant loaded with slow release antibiotic of the invention improves anti infection capacity,ensures continuity of implanted bone and repaired bone in period I after debridement or focus clearing of bone defect caused by open fracture and other reasons and also can prevent tubercle germ infection. PDLLA can be well absorbed by organism. Slow-release antibiotic can maintain release for a period of time, thus antibiotic effective concentration in local part can be maintained for a long time, and side effect of whole body medicine application is avoided while infection is effectively prevented and treated at the local part.

The invention relates to an antibiotic, and a preparation method and an application thereof. The preparation method comprises the following steps: activating Lactococcal lactis ATCC 11454, picking single colony, culturing, centrifuging to remove bacterial strains to obtain a fermentation broth, adjusting the pH value of the fermentation broth to 2, carrying out rotary evaporating concentration, adjusting the pH value of the obtained concentrate to an initial value, centrifuging, taking the obtained supernatant, and filtering to obtain a fermentation concentrate; and mixing the fermentation concentrate with a PBS buffering solution, adding a double water phase system formed by Triton X-114 and MgSO4, centrifuging to realize phase balance of double water phases, taking the obtained upper phase, and diluting the upper phase by using the PBS buffering solution to prepare the antibiotic containing nisin and Triton X-114. The antibiotic has a substantial bacteriostasis effect on Gram-positive bacteria. The antibiotic includes nisin and Triton X-114, and the combination of the nisin and the Triton X-114 makes the antibiotic have high titer. The preparation method has the advantages of simple extraction process and low cost, and is suitable for intermittent and large-scale production and processing of high-tilter and high-yield antibiotics.

The present invention relates to a preparation method and application of an antibiotic preparation used in human medicine and veterinary medicine for treating local microbial infections of hard tissue and soft tissue. The preparation method of the antibiotic preparation of the present invention is as follows: from water and an amphoteric component such as an alkyl sulfate, and one or more antibiotics selected from the group consisting of aminoglycoside antibiotics, lincosamide antibiotics, and tetracycline antibiotics The components, an organic auxiliary component and / or an inorganic auxiliary component, and if necessary, at least one biologically active auxiliary component are mixed with each other, and can be further made into molded bodies, granules, and powders. , film, felt and thread.

The invention belongs to the technical fields of molecular biological techniques and clinical tests, and particularly discloses a nosocomial infection multiple causative agent parallel detection gene chip and a preparation method and application thereof. The gene chip can be used for rapidly and sensitively detecting fourteen medical treatment infection typical causative agents such as acinetobacter baumannii, enterobacter cloacae, enterococcus faecalis and the like by using one reaction, and the detection data of coinfection of yeast and bacterium which cannot be provided by a traditional amplification method is offered. Furthermore, the gene chip also comprises a methicillin resistance target spot, thus realizing further detection of staphy lococcus infection, therefore, a clinician can realize which causative agent a patient is infected in one or two hours, and realize whether the patient has drug resistance, so as to decide whether the patient needs antibiotics or which antibiotics is needed, so that the gene chip has a greater clinical guiding significance.

The invention relates to a slow-releasing agent of antibiotics containing aminoglycoside, comprising slow-releasing micro-sphere containing slow-releasing findings and aminoglycoside antibiotics, and special dissolvent containing suspending adjuvant agent such as sodium carboxymethyl cellulose with the adhesive degree being 100cp-3000cp (at 20-30 Deg. C). The slow-releasing findings comprises EVAc, polyphenyl, PLA, PLGA, decanedioic acid copolymer, albumen glue and gelatin; the slow-releasing micro-sphere can be produced to slow-releasing implanting agent and unguent agent. When the slow-releasing implanting agent and injection is placed or injected into bacteria, the medicine can be released slowly for more than 5-30 days, and toxicity is dramatically reduced at the same time when effective medicine concentration is got and maintained. The said slow-releasing agent is specially effective for chronic medullitis, bedsore, intractable ulcer on skin, diabetes femoral head necrosis and other abscessus, which are caused by Staphylococcus, Streptococcus, Streptococcus, acne Propionibacteriaceae, Enterobacter, Enterobacter, gonotoxin or parameningococcus.

The invention discloses Peptaibol antibacterial peptide compounds and a preparation method and application thereof. The four novel Peptaibol antibacterial peptide compounds are separated and obtained from acremonium persicinum SC0105 fermentation products, and it is shown through antibacterial tests that the antibacterial peptide compounds have a remarkable inhibition effect on staphylococcus aureus (MSSA) and methicillin-resistant staphylococcus aureus (MRSA). Peptaibol antibacterial peptide is a main metabolic product of a strain, the yield is high, and high research and development potential is achieved. The Peptaibol antibacterial peptide can serve as an antibiotic substitute and be applied to medicine for preparing antibacterial drugs and feed additives in the breeding industry.

The invention belongs to the technical field of medicaments and in particular relates to oxazolidinone bacteriophage containing azaheterocycle, pharmaceutically-acceptable salts or stereoisomeride thereof, wherein the oxazolidinone bacteriophage is shown as a general formula (I) in the specification, wherein R1, R2, R3, R4, R5, R6, R7, R8, R9, X, Y and Z are defined as a description. The invention also relates to a preparation method of the compound, a medicinal composition containing the compound as well as application of the compound or the medicinal compound thereof to preparation of medicines for treating and / or preventing infectious diseases.

The invention discloses an efficient florfenicol powder composition and a preparation method thereof. The powder composition comprises the following components in percentage by weight: 1-50% of florfenicol, 46-98.9% of a florfenicol-absorbed carrier and 0.05-4% of a florfenicol absorption promoter. The method comprises the following steps of: preparing the raw materials according to the above components; preparing a florfenicol organic solvent solution; dissolving the florfenicol absorption promoter and the florfenicol-absorbed carrier into water to obtain a mixed water solution; and adding the florfenicol organic solvent solution into the mixed water solution, and rapidly spray-drying. The preparation method disclosed by the invention is simple and low in cost. The product disclosed by the invention has the advantages of extrinsic quick release, esoteric quick absorption, high plasma peak concentration, thorough drug absorption and less antibiotic residue. The product disclosed by the invention can be applied to the treatment of infection on all parts of domestic animals caused by bacteria.

The invention discloses original-quality ceftriaxone sodium and a pharmaceutical preparation thereof. The key technology and industrialization of the third generation of cephalosporin antibiotics active ester intermediate wins the second prize of National Scientific and Technological Progress Award, and the third generation of cephalosporin antibiotics intermediate AE active ester is a key factor for affecting the internal quality of the ceftriaxone sodium. A preparation method comprises the steps that 1, boron trifluoride-acetonitrile serves as a catalyst, and on the condition that acetonitrile serves as solvent, a triazine ring is reacted with 7-ACA to generate 7-ACT; 2, triethylamine and aminothiazoly loximate are added into the solvent, a chloroformate activator is dropwise added slowly during cooling mixing, the 7-ACT is added for a one-pot reaction after stirring is conducted, and ceftriaxone is obtained; 3, a salt-forming agent is added, and the ceftriaxone sodium is obtained. According to the preparation method, use of a condensing agent with higher price is avoided, the process route is shortened, operation is easy, the reaction condition is mild, the product yield is high, the purity is good, and industrial production is easy.

Ulifloxacin has low water solubility and low bioavailability. The invention relates to an Ulifloxacin salt, a preparation thereof, a kit having the medicinal antibiotic preparation and the use of the new Ulifloxacin salt in the preparation of an anti-bacterial-infection medicine. The invention particularly relates to a new medicinal preparation which is used for injection and can be stored stablyand the chemical name of which is 6-fluoro-1-methy-7-(1-piperazinyl)-4-oxo-4H-[1,3]thiazeto[3,2-alpha]quinoline-3-carboxylate. In the invention, the medicinal preparation is administrated by direct injection in form of thiabutyldine quinoline carboxylate, the solubility is improved, the bioavailability is improved and treatment effect is enhanced.

The invention discloses a process for preparing board-spectrum antibiotics by immune induction biotechnology, and particularly relates to a process for preparing board-spectrum antibiotics by immune induction biotechnical synthesis, which employs super immunoprotein synthesis ability of silkworms and includes: allowing fifth-instar silkworms to synthetize high-quantity sericin antibacterial peptides from a middle division of silkgland and silk fibroin antibacterial peptides from a posterior division of the silkgland; regulating silk protein genes with highly bioactive agent; and performing synthesis and amplification to obtain board-spectrum antibiotics by solid-phase and liquid-phase biotechnology. By the process, biotechnical synthetic routes are simple and easy to learn, production cost is low, productivity is highly controllable, disease resistance reaches more than 80%-95%, and achievement is easy to transform and convenient for industrial production. The antibacterial peptides prepared by the process can be mixed with oil and used for soaking roots, and have a higher bacterial effect and efficient and broad-spectrum antibacterial advantages to infections caused by wilt fusarium, gram-positive bacterium, gram-negative bacterium or pathogens such as Bacillus subtilis, bacterial wilt and the like.

The invention discloses original development quality ceftazidime and a medicine preparation thereof. The third-generation cephalosporin antibiotics active ester midbody key technology and industrialization obtains the second prize of National Scientific and Technological Progress Award. The cephalosporin antibiotics active ester belongs to a key factor for influencing the internal quality of the cephalosporin. A preparation method comprises the following steps that (a) mixed solvents are added into ceftazidime side chain acid, dibenzothiazyl disulfide, aniline and 2-picoline; triethyl phosphate is dripped for reaction; (b) a coarse product is refined to obtain ceftazidime side chain acid active ester, and the first mother liquid is recovered; (c) the material is added into a mixed solvent for neutralizing 7-APCA; triethylamine is dripped; the temperature reduction is performed for crystal separation and filtering to obtain ceftazidime tert-butyl ester; the second mother liquid is recovered; (d) the ceftazidime tert-butyl ester is subjected to hydrolysis and purification, and then, the ceftazidime is obtained. The original development quality ceftazidime has the advantages that high-toxicity triphenylphosphine is not used; waste liquid and waste slag can be sufficiently recovered and reutilized; the method is safe; the cost is low; the yield is high; the industrial production is facilitated.

The present invention relates to an antibiotic-polymer complex that ensures continuous release of antibiotics for several days under physiological conditions and can be used in human and veterinary medicine. The antibiotic-polymer composite of the present invention is characterized in that it contains one or more hydrophobic polymers selected from the group consisting of polymethacrylates, polyacrylates, methacrylate-acrylate copolymers and a Or in a homogeneous mixture of polymers composed of hydrophilic polymers such as polyethers, one or more substances selected from the group consisting of aminoglycoside antibiotics, lincosamide antibiotics, tetracycline antibiotics, and quinolone antibiotics are suspended in water. A poorly soluble antibiotic, a water-soluble antibiotic selected from aminoglycoside antibiotics, lincosamide antibiotics, β-lactam antibiotics and tetracycline antibiotics as needed, and an organic auxiliary as needed material and causing the suspension to form a composite.

The invention provides a plant antibiotic compound spraying agent and a preparation method thereof. The plant antibiotic compound spraying agent is mainly composed of gastrodia tuber, large-flowered skullcap root, Yedo violet, Sophora flavescens, broom cypress fruit, Cotex Dictamni, calyx melo, pomegranate rind, garden burnet, surfactant, cosurfactant and the like. The plant antibiotic compound spraying agent is an external skin preparation with substantial curative effect and capacity of eliminating recurrence, is free of any western medicine and poses no pollution and residual toxicity to human beings and the environment. Clinical test results show that the plant antibiotic compound spraying agent has an action rate of 95% or above and a total effective rate of 99% or above. Through synergism of all the raw materials, the plant antibiotic compound spraying agent has good antibacterial and anti-inflammation effect, is free of irritation to skin and prevents occurrence of allergy. The plant antibiotic compound spraying agent can be used for treating beriberi, burns and scalds, and acne and exerts good curative effect on tinea, scabies and skin diseases of other parts of the body caused by fungi.

The present invention discloses a novel antimicrobial peptide and an application thereof in citrus preservation. A linear tetrapeptide which has a strong inhibition effect on rotten fungi after citrusharvesting is separated out from fermentation liquid of penicillum oxalicum SG-4 by steps of ethanol precipitation, concentration and freeze-drying, high performance liquid chromatography, etc. Aftermagnetic resonance imaging, high-resolution mass spectrometry and other spectrum analysis, the substance is found to be a propylene glycol monomethyl ether connected linear 4-peptide compound Thr-Thr-Val-Ser (propanediol tetrapeptide, PTP for short), parts of peptide bonds and alpha-amino groups show methylation, and structures and functions of the type of the compound are not reported internationally. Experiments show that the PTP has the strong inhibition effect on rotten fungi penicillium italicum after the citrus harvesting, antimicrobial functions also exist when PTP is at a low concentration of 1 [mu]g / mL, and the effect of the PTP is significantly better than commonly used citrus preservatives of bellkute, a prochloraz and imazalil compound, carbendazim, etc. in the market. The antibiotic is a potential citrus preservative and can be used on a large scale in industrial and agricultural production.

The present invention belongs to the technical field of medicine, and specifically relates to an oxazolidinone antibiotic containing a five-membered heterocycle represented by general formula (I), a pharmaceutically acceptable salt thereof or a stereoisomer thereof: wherein R1, R2, R3, R4, R5, R6, R7, R8, A, X or n are as defined in the description; the present invention also relates to the preparation method of these compounds, the pharmaceutical composition containing these compounds, and the preparation of these compounds in the treatment and / or prevention of infection Use in medicine for diseases.

The invention discloses a preparation method of a Chinese medicine irrigation solution for treating urethral orifice burning type senile vaginitis, belonging to the technical field of preparation method of Chinese medicines. At present, generally, antibiotics and sulfanilamide medicines are used for treating urethral orifice burning type senile vaginitis, and the disadvantage is large toxic and side effect. The Chinese medicine irrigation solution disclosed herein is prepared by putting 38 kinds of herbs consisting of tartarian buckwheat root and rhizome, dandelion, dysosma versipelis, wikstroemia indica, saururus chinensis, radix berberidis, folium isatidis, garden euphorbia herb, Pennywort Herb, Rohdea japonica Roth, Aster ageratoides, radix sophorae tonkinensis, edible tulip, senecio scandens, Huperzia serrata, Japanese stephania root, thymifoious euphorbia herb, meadowrue root and rhizome, purslane, leaf of Chinese coriaria, ardisia crenata, centella asiatica, Polygonum hydropiper, sugarcane, HlblSClls llutabilis, sophora flavescens, balsam pear, ilex latifolia thumb, Elephantopus scaber, polygonum cuspidatum, saxifrage, folium phyllostachys and liquorice in water for soaking,then decocting with slow fire, filtering and removing the residues to obtain a liquid medicine. The Chinese medicine irrigation solution disclosed herein has the advantages of small toxic and side effect, short treatment course and high cure rate.

The invention belongs to the technical field of organic synthesis and pharmaceutical chemistry and especially relates to a preparation method of an animal antibiotic tulathromycin. According to the preparation method, azithromycin A and acetic anhydride which are used as raw materials are subjected to protection and oxidation to obtain oxide cyclic ketone; the intermediate is used for innovative addition of cyclic ketone and nitromethane; an addition product is reduced to obtain methyleneamine; direct condensation between methyleneamine and propionaldehyde is carried out; and reduction is conducted to obtain the high-purity target compound. Raw materials which are cheap and easily available are adopted. The preparation method has advantages of simple reaction, easily-controlled reaction process, high product purity, manageability, high yield, low cost and the like, and is suitable for large-scale industrial production.

The invention belongs to the technical field of dairy processing, and specifically relates to a sugar-free yoghurt and a preparation method thereof. The yoghurt contains a raw buffalo milk component, a momordica grosvenori extract, lactobacillus bulgaricus and streptococcus thermophilus. The preparation method for the yoghurt comprises the following steps: a) raw buffalo milk pre-treatment: taking raw buffalo milk, and carrying out an antibiotic test and a standardization pre-treatment; b) sterilization: mixing and stirring filtered liquid glucose with the pre-treated raw buffalo milk to achieve a homogeneous state, and then sterilizing; c) strain preparation: taking a strain and carrying out enlargement fermentation and production fermentation to obtain a production fermentation agent; d) inoculation and fermentation: inoculating the production fermentation agent into a sterile emulsion, filling, sealing, fermenting and cooling to obtain a sugar-free momordica grosvenori yoghurt product. The yoghurt product obtained by the preparation method disclosed by the invention is excellent in the performances of colour state, various indexes and the like, simple in preparation method, low in production cost, and capable of being eaten by consumers who are not allowed to eat sugar.

The invention discloses a preparation method of a Chinese medicine irrigation solution for treating frequent micturition and urgent micturition type trichomonas vaginitis, belonging to the technical field of preparation method of Chinese medicines. At present, generally, antibiotics and sulfanilamide medicines are used for treating frequent micturition and urgent micturition type trichomonas vaginitis, and the disadvantage is large toxic and side effect. The Chinese medicine irrigation solution disclosed herein is prepared by putting 25 kinds of herbs consisting of pericarpium arecae, nericum indicum, waxgourd peel, phaseolus calcaratus, Zanthoxylum bungeanum Maxim, stigma maydis, bottle gourd peel, dayflower, Polygonum aviculare, pink herb, caulis akebiae, Tetrapanax papyriferus, junci medulla, shepherdspurse herb, Convallaria majalis, mole cricket, sophora flavescens, balsam pear, ilex latifolia thumb, Elephantopus scaber, polygonum cuspidatum, saxifrage, Tetrapanax papyriferus, daylily root and licorice in water for soaking, then decocting with slow fire, filtering and removing the residues to obtain a liquid medicine. The Chinese medicine irrigation solution disclosed herein has the advantages of simple preparation, small toxic and side effect, short treatment course and high cure rate, and can avoid the adverse effects of applying western medicines.

Compositions containing polyether antibiotics having reduced particle size and surface-acting agents are useful for protecting untanned animal hides against decomposition for up to 96 hours when stored at ambient temperature. Further processing of treated hides presents no environmental hazards. The present invention is useful in the preservation of hide quality of a wide variety of animals useful for tanning.

The invention discloses a Pseudomonas aeruginosa vaccine and a preparation method thereof, which relate to a vaccine and a preparation method thereof. The invention solves the problems that the prior antibiotic is difficult to cure Pseudomonas aeruginosa infection, and a Pseudomonas aeruginosa vaccine with single valence can not satisfy the demand of clinical immunity. The vaccine consists of endotoxin of Pseudomonas aeruginosa P1, endotoxin of Pseudomonas aeruginosa P2, endotoxin of Pseudomonas aeruginosa P6 and toxoid of Pseudomonas aeruginosa P103 which are placed in physiological saline. The preparation method comprises the following steps: the endotoxins of Pseudomonas aeruginosaes are prepared; the toxoid of the Pseudomonas aeruginosa P103 is prepared; and the Pseudomonas aeruginosa vaccine can be obtained by mixing the endotoxins of the Pseudomonas aeruginosaes and the toxoid of the Pseudomonas aeruginosa P103. The Pseudomonas aeruginosa vaccine belongs to a polyvalent vaccine; the endotoxins of the Pseudomonas aeruginosa P1, the Pseudomonas aeruginosa P2 and the Pseudomonas aeruginosa P6 are adopted, so known type 11 Pseudomonas aeruginosa can be immunized; and the toxoid of the Pseudomonas aeruginosa P103 is added, so the immune effect of the vaccine to the Pseudomonas aeruginosaes is strengthened.