The invention relates to a manufacture device and method of a hydrological coupling test three-dimensional fracture rock sample; the manufacture method comprises a three-dimensional fracture precision positioning sample mold and a high-pressure injection fracture preforming device; the three-dimensional fracture precision positioning sample mold comprises side plate I, side plate II, a bottom plate and positioning porous plates and can provide precision positioning for multiple three-dimensional fractures and forming of a sample. The high-pressure injection fracture preforming device comprises polyester film boxes, cotton threads, rubber water pipes, mandrels and manual screws, and can preform high-pressure injection three-dimensional fractures and form an injection passage. By combining annularly distributed holes and transversely distributed holes, the positioning porous plates are used to allow precision positioning for the angle, spacing and spatial positions of the multiple three-dimensional fractures; the small-diameter rubber water pipes and mandrels to preform hydraulic passages in the sample and communicate the open fractures, high-pressure injection of water is achieved for the open fractures. The manufacture device and method are simple in structure, convenient to operate and good positioning precision and are widely applicable to the hydraulic coupling test for three-dimensional fracture rock materials.