The invention relates to the field of essences, and particularly discloses a floral and fruity flavoring essence, a preparation method and application thereof. The floral and fruity flavoring essence comprises the following components: white flower alcohol, polysantalol, tetrahydrogeraniol, cis-4-Isopropylcyclohexylmethanol, methyl dihydrojasmonate, ambrotone, cis-jasmone, methylcyclopentadecenone, 2,2,5-trimethyl-5-pentylcyclopentanone, ligustron, Convallaria majalis ketone, methyl pamplemousse, hydroxyl vanillyl alcohol, vetiver acetate, bay leaf oil, a fixative, a modifier, a mixture, and a solvent. The preparation method comprises the following steps: weighing the components according to the proportion, mixing the components, refluxing and stirring, and standing for 24-48 hours to obtain the floral and fruity flavoring essence. According to the invention, the floral and fruity flavoring essence can be used in perfume, shampoo, shower gel and laundry detergent, and has the advantages of high coordination degree of fragrance and comfortable fragrance; and the preparation method has the advantages of being simple and convenient.