The invention discloses a burley tobacco flavor enhancing preparation, which comprises the following ingredients in percentage by mass: 10 to 15 percent of carbohydrates, 1 to 1.5 percent of organic acid, 0.6 to 1.2 percent of neutral protein enzyme and the balance of water, wherein the enzyme activity of the neutral protein enzyme is 100 thousand U / g. The burley tobacco flavor enhancing preparation provided by the invention is favorable for improving the exquisite degree of smoke gas, the intensity is reduced, the impurity odor is covered, the drying degree is lightened, and the aftertaste is improved, wherein the organic acid is added, saccharides can be converted and are absorbed by tobacco leaves, the organic acid and amino compounds can take reaction to generate various kinds of flavor substances, and the effect of enhancing the cigarette flavor is realized; during the burning, the carbohydrates per se can be coked, and the graceful and comfortable coked sweet and fragrant effect is generated; and the protein content in the tobacco leaves can be degraded. The preparation has the advantages that the use is convenient, the preparation is only needed to be sprayed to the tobacco leaf surface, then, the operation is carried out according to normal burley tobacco maturity harvesting standards and a preparation method, the cost is low, the effect is good, and the practicability is high.