131results about How to "Effective infection" patented technology

The invention discloses a manufacturing method of Chinese caterpillar fungus, which is characterized by the following: establishing greenhouse booth moth culturing field according to culturing temperature and moisture; reducing the danger of natural enemy of moth to accelerate weak plant root of herb and bush; releasing certain quantity of moth fertilized ovum, larva, pupal or and imagine in the culturing field; elevating moth larva breeding density with 110-3500000 per hectareha; breeding the moth from one year to 6-8 years within 365-425d; forming sac fungus; collecting 530000-1570000 Chinese caterpillar fungus per hectareha.

The invention discloses an EV-71 virus species, a human inactivated vaccine and a preparation method thereof. The classification name of the virus species is intestinal virus 71 with a storage number of CGMCC No.2701. The prepared human EV-71 inactivated vaccine comprises the following components: 100 Mu g / ml of EV-71 alive purified antigen, 0.8-1mg / ml of aluminum hydroxide, and 0.05-0.1mg / ml of merthiolate. The experiments show that the vaccine products have good immunogenicity and safety.

This invention relates to cyclosporin derivatives of general formula (I) wherein A, B, R<1>,R<2> and X are as defined in the specification, and pharmaceutical compositions prepared from the same, for use in treatment of hepatitis C virus.

The invention belongs to the technical field of medicines and particularly relates to a new function of micromolecular metabolin, namely glutamine. The micromolecule can improve the sensitivity of bacteria including drug-resistant bacteria to antibiotics so as to solve the problem of drug resistance of bacteria. The micromolecule provided by the invention has better effect and higher safety and operability compared with antibiotic which is applied to the medicament for resisting drug resistance of bacteria at present.

The invention relates to a Coxsackie A16 type virus mutant strain capable of effectively infecting mice. In the invention, a mouse-adaptive mutant virus strain is unexpectedly obtained through continuous passage of CA16 virus by using the mouse cells and mouse; the mouse-adaptive strain has very strong mouse infection ability and can be used for preparing a mouse model of Coxsackie virus infection so as to study the pathogenic mechanism of the virus, evaluate the antiviral drugs, etc.

The invention discloses a porcine circovirus-like particle, a vaccine and a preparation method thereof. The virus-like particle is composed of a main structural protein-nucleocapsid protein of 2-type porcine circovirus, can excite cell and humoral immune response, can be used as a virus-like particle vaccine (VLP vaccine) to immune different fauna and can safely and effectively prevent PCV-2 infections after being used. The VLP can be made into injections, nose drops and drinking preparations by adding adjuvants or not. An ideal vaccine is provided for security of different populations of sows, piglets, fattening pigs and for effective immune prevention and control of PCV-2 infections.

The invention relates to an application technology of AM mycorrhizal fungi in virus-free potato test tube plantlet transplanting. The technology comprises the following steps of: preparing an inoculant containing fungal spores, external mycelia, an infected clover root system and a river sand mixture by taking clovers as host plants; then preparing a transplanting substrate; and adding the inoculant to an interlayer of the substrate with a proportion of 3,000-5,000 spores per kilogram of soil. By adopting the method to transplant, the output of minituber virus-free potatoes can be increased by more than 25 percent.

The invention discloses a charge modified lactoferrin and carrageenin combination medicine which comprises 0.01 part by weight of lactoferrin, 1.5 parts by weight of carrageenin, 5 parts by weight of carbomer, 50 parts by weight of glycerol and 0.1 part by weight of ethylparaben. The combination medicine is one of a gel, a suppository and a dressing. The combination medicine disclosed by the invention can be used for preventing and treating female human papilloma virus infection as well as preventing and treating female gynecological gram-negative bacterium infection.

The invention provides an establishing method of a recoverable immortalized hepatic cell line. The establishing method comprises the following steps: separating a hepatic progenitor cell from liver in a mouse which is 12.5-14.5 days old in an embryonic period; guiding a retrovirus containing genes SV40T and HSV-TK into the hepatic progenitor cell; screening a monoclonal cell strain having a hepatic progenitor cell marker and having a function of differing to a mature hepatic cell; then guiding a retrovirus containing CD gene into the cell strain so as to obtain recoverable immortalized hepatic cell carrying double suicide genes. The cell strain can multiply in vitro to obtain the phenotype and functions of a normal hepatic cell, the safety of the immortalized cell becomes adjustable through controllable modes such as locus recombination and drug screening; when the cell is used in a human body, the biological safety of the immortalized cell is ensured to the greatest extent and the dangerousness of the immortalized cell is reduced; therefore, a reliable, safe and ideal hepatic cell material is provided for bioartificial liver technology.

The invention discloses valnemulin hydrochloride emulsion for animals. Each 100ml of emulsion mainly comprises the following components by weight: 5 to 10g of valnemulin hydrochloride, 1 to 5g of emulsifier, 20 to 40g of oil for injection, 5 to 15g of ethanol and the balance of water for injection. The valnemulin hydrochloride emulsion for animals has the advantages of quick in vivo distribution and good target, can reduce the medicament residue in vivo and the toxic or side effect on the animals, and also can mask the unpleasant odor of the medicament, and improve the stability of the medicament. The valnemulin hydrochloride emulsion for animals can effectively treat gram-positive bacteria and mycoplasma infection, particularly swine dysentery and porcine enzootic pneumonia, and can reduce the using amount of the medicament and avoid medicament tolerance.

The invention discloses a grass carp reovirus S11 gene eukaryotic expression recombinant plasmid preparation method and application thereof in serving as nucleic acid vaccine, and belongs to the technical field of gene engineering and molecular immunology. The preparation method disclosed by the invention is characterized by comprising the steps of extracting viral genome RNA, performing reverse transcription to convert the viral genome RNA into cDNA, amplifying a corresponding DNA sequence out, constructing the DNA sequence to pcDNA-3.1(+) plasmid, converting Escherichia coli DH5alpha, screening out positive clone bacteria containing recombinant plasmid, culturing a lot of the positive bacteria and extracting recombinant plasmid S11-pcDNA3.1 contained in the bacteria. The recombinant plasmid is utilized as nucleic acid vaccine to perform intramuscular injection on the grass carps, and the nucleic acid vaccine enters the muscle cells and expresses VP35 protein of the grass carp reovirus in the muscle cells; thus, fish body immune cell proliferation is stimulated, antiviral related gene expression is up regulated, fish bodies are stimulated to generate antiviral antibodies, and capability of grass carps in resisting grass carp reovirus infection is effectively improved; furthermore, the grass carp reovirus S11 gene eukaryotic expression recombinant plasmid can be used for preventing a grass carp hemorragic disease caused by the grass carp reovirus in aquaculture.

The invention discloses a plant anti-allergy skincare essence, which is characterized by comprising the following components in percentage by weight: 0.5 to 3% of plant anti-allergy agent and the balance being basic skincare essence; wherein the plant anti-allergy agent is extracted from one or more of green tea, centella, licorice, chamomile, gentrin knotweed, rosemary, and radix scutellariae. The invention also discloses a preparation method of the plant anti-allergy skincare essence. Through tests and screening, people find that stock solution extract from seven raw materials: green tea, centella, licorice, chamomile, gentrin knotweed, rosemary, and radix scutellariae can effectively prevent allergy and inflammation, and moreover, the provided plant anti-allergy skincare essence does not contain any harmful additive and is stable.

The invention discloses a DNA (Deoxyribose Nucleic Acid) vaccine of an HCV (Hepatitis C Virus) and a preparation method thereof. The vaccine has CTL (Cytotoxic Lymphocyte) cell immunity and the capability of neutralizing virus infection. The preparation method comprises the following steps of: constructing a recombinant plasmid containing an E1E2 wild-type gene; and mutating a corresponding glycosylation mutant (E1-N209SS is mutated into E1-D209SS, and E2-N430AS is mutated into E2-D430AS) on site-directed mutated PCR (Polymerase Chain Reaction) mutated E1E2; and coupling a CpG sequence by adopting a PCR method, wherein a mutant is named CpG-N2N8. After a mouse is immunized with the DNA vaccine, an antiserum for neutralizing HCVcc (cell cultured Hepatitis C Virus) can be obtained. The method is easy and convenient, and is low in cost; and the DNA vaccine can be used for expressing virus genes, can be used for inducing body fluid immune response of a CTL cell immunizing and neutralizing antibody and which is stronger than wild E1E2, and has a remarkable economic benefit.

The invention relates to veterinary valnemulin, a novel liposome formulation of the salt thereof and a preparation method thereof, which belongs to the field of veterinary antibiotic preparations. In the preparation, valnemulin and the salt thereof serve as the main components, phospholipid and cholesterol serve as membranes, and the common liposomes, the lung targeting liposomes or the long circulating liposomes of valnemulin or valnemulin salt can be prepared. The valnemulin and the liposomes of the salt thereof have better tissue selectivity compared with the common preparation, are effective to the bacterial and mycoplasma infection in cells, and can reduce the toxic and side effect. The lung targeting liposomes targets the medicine to the lung, and has significant effect to the mycoplasma infection of the lung, bacterial infection and the like, and reduces the toxic and side effect. The long circulating liposomes significantly improves the circulation time of the liposomes in thebody, so that the medicines displays the efficacy for a longer time more effectively, so s to reduce the delivery times and the dosage.

The invention successfully screens a virus strain VR18 suitable for building an animal model of CA16 infection and builds the animal model of the CA16 virus infection by infecting a suckling mouse with the virus strain VR18. The invention also relates to a method for building the animal model of the CA16 injection and the use of the virus stain VR18 in the building of the animal model of the CA16infection and the like. The method provides valuable experiment data for study on CA16 vaccines.

The invention discloses an application method for replacing antibiotics with microbic muramidase in feed. The excellent effect can be obtained by controlling the volume of addition at 60 to 400g / ton feed; and the volume of addition in the feed of different animals is relatively adjusted. By using the method, the inflammation and the infection can be resisted effectively by using the excellent effects of antibiosis and sterilization of the muramidase, the effects of antibiosis and sterilization also have the strong killing effect on bacterial strains generate drug resistance on antibiotics, and the effects of antibiosis and sterilization can ensure that the health of intestinal canals of the animals, prevent and cure the diarrhea of the animals and improve the production performance of the animals. The application method has the advantages of good curative effect, high safety, low cost, no drug resistance, no drug residue and no toxic or side effect, and the microbic muramidase is a green environment-friendly active substance.

The invention discloses a solenopsis invicta and termite touch killing powder containing ethofenprox, aiming to provide a safe and efficient solenopsis invicta and termite touch killing powder containing ethofenprox. The invention comprises the following components in percentage by weight: 0.01-0.1% of ethofenprox, 3.0-15.0% of french chalk, 1.0-1.5% of warning colouration powder and 83.40-95.99%of maize starch. The solenopsis invicta and termite touch killing powder containing ethofenprox of the invention is safe to mammals, aquatic organisms, honeybees and natural enemies, and has favorable control efficiency for solenopsis invicta and termite, is a safe and efficient medicament, can be widely applied in various environments and ecosystems for preventing and curing solenopsis invicta and termite and has large popularization and application value.

The invention belongs to the technical field of medicines. The invention relates to a pharmaceutical composition, in particular to a pharmaceutical composition for preventing and treating COVID-19 (diseases caused by novel coronaviruses) and a preparation method thereof. The pharmaceutical composition comprises active ingredients of mycobacterium vaccae for injection and salbutamol sulfate, and the weight ratio of the mycobacterium vaccae for injection to the salbutamol sulfate is 1: (50-200). The medicine prepared from the medicine composition can adopt an aerosol inhalation treatment mode, and through short-term aerosol inhalation, the compliance of a patient can be improved by providing the medicine with a specific purpose for the patient, so that the patient can take the medicine conveniently, and the medical cost is reduced. The pharmaceutical composition for preventing and treating COVID-19 disclosed by the invention has the advantages that the COVID-19 can be effectively prevented and treated; severe or critical symptoms of patients are relieved, nucleic acid negative conversion time is shortened by about 4 days averagely, treatment time is correspondingly shortened, other respiratory virus infections can be prevented and treated nonspecifically, and a new way and scheme are provided for clinical prevention and treatment of COVID-19 and respiratory virus infections.

The invention discloses a compound bergamot oil nano-emulsion composition, which is characterized in that: the granularity of the nano-emulsion is 1nm to 100nm, and comprises the following substances by weight mass: 23 to 35 percent of surface active agent, 0.3 to 8 percent of cosurfacant, 1.5 to 10 percent of bergamot oil, 0.5 to 5.5 percent of patchouli, 0.4 to 4.5 percent of tea tree oil, 0.3 to 2.2 percent of pennyroyal and distilled water, wherein the sum of the mass percentage of the ingredients is 100 percent. The compound bergamot oil nano-emulsion composition is broad-spectrum antimicrobic, has good treatment effect on eczema, psoriasis, herpes, dermatitis, blain, wound, acne, urinary infection and the like, the permeability is strong, the stability is good, content of active ingredients is high, transdermal absorption effect is remarkable, action time is durable, the preparation method is simple and is easy to popularize and use, and the medicine has vast application prospect on the medical market.

The invention relates to veterinary valnemulin, a novel liposome formulation of the salt thereof and a preparation method thereof, which belongs to the field of veterinary antibiotic preparations. In the preparation, valnemulin and the salt thereof serve as the main components, phospholipid and cholesterol serve as membranes, and the common liposomes, the lung targeting liposomes or the long circulating liposomes of valnemulin or valnemulin salt can be prepared. The valnemulin and the liposomes of the salt thereof have better tissue selectivity compared with the common preparation, are effective to the bacterial and mycoplasma infection in cells, and can reduce the toxic and side effect. The lung targeting liposomes targets the medicine to the lung, and has significant effect to the mycoplasma infection of the lung, bacterial infection and the like, and reduces the toxic and side effect. The long circulating liposomes significantly improves the circulation time of the liposomes in the body, so that the medicines displays the efficacy for a longer time more effectively, so s to reduce the delivery times and the dosage.

Streptomyces amritsarensis N1-32, a micro-ecological preparation thereof and a preparing method thereof are provided. The Streptomyces amritsarensis N1-32 is deposited in the China Center for Type Culture Collection with an accession number of CCTCCNO:M 2019381. The Streptomyces amritsarensis N1-32 can effectively suppress growth of common fish pathogenic bacteria, especially Aeromonas veronii. Application of the micro-ecological preparation for grass carp liver cells proves that the Streptomyces amritsarensis N1-32 is harmless and nontoxic to fish. The micro-ecological preparation is utilizedas feed to feed grass carp, crucian, and other types of freshwater fish, and a result shows that the growth rate and the feed efficiency of the grass carp are increased, and immunity of freshwater fish is boosted, and therefore, resistance of freshwater fish, such as grass carp, to fish pathogenic bacteria can be significantly improved.

The invention relates to Coxsackievirus B5 CV-B5 CVB5 / JS417 capable of being stably copied and abdicated. The preservation number of the virus is CGMCC (China general microbiological culture collection center) No. 13851. The invention further relates to an application of Coxsackievirus B5 CV-B5 the CVB5 / JS417 in preparing a Coxsackievirus B5 CV-B5 infected mouse. The experimental results show that obvious eosinophilic necrosis and vacuolar degeneration necrosis in spots of the brain, spinal cord, cardiac muscle and skeletal muscle of hind limbs of the Coxsackievirus B5 CV-B5 infected mouse can be seen, and meanwhile, immunohistochemistry shows that positive or strong positive reaction appears in brain and spinal neural cells and cardiac muscle and skeletal muscle cells of the hind limbs, which verifies that the CVB5 / JS417 can cause obvious lesions and necrosis to the brain, spinal nerves, cardiac muscle and skeletal muscle of the hind limbs of the infected mouse.

The invention relates to a vaccine composition. The vaccine composition comprises an immunological dose of egg drop syndrome virus Penton protein or an immunological dose of a live vector containing arecombinant egg drop syndrome virus Penton protein gene, and a veterinary acceptable carrier. The vaccine composition can effectively protect the occurrence of egg laying of chickens, and can completely prevent against wild strains of egg drop syndrome virus in different regions.

A Chinese veterinary medicine for treating the colibacillosis of fowls is prepared from 4-14 Chinese-medicinal materials including rhubarb, scutellaria root, coptis root, phellodendron bark, etc.

The invention discloses a wet tissue preventing the hand-foot-mouth disease and a preparation method thereof, and relates to the technical field of sterilizing wet tissues. The wet tissue preventing the hand-foot-mouth disease comprises non-woven cloth and a solution on the non-woven cloth. The solution contains artemisinin, goldthread roots and a mint extract. The preparation method includes the steps that the goldthread roots are boiled firstly to obtain medicine liquid, the medicine liquid is filtered to obtain a filtered solution, then the artemisinin and the mint extract are dissolved in ethyl alcohol, the liquid is mixed and diluted by adding water, and finally the non-woven cloth adsorbs the diluted solution according to the specification. The wet tissue can safely, conveniently and effectively prevent the hand-foot-mouth disease of children.

The present invention relates to phages isolates having a strong lytic activity against Enterobacter sakazakii strains and their use as anti-microbial agent in food products, in particular infant formula, and for sanitation of factory environments. The invention also relates to food compositions and anti-microbial agents prepared thereof.

The invention relates to genetic engineering expression and preparation methods of cecropin LL-37 and application thereof. The preparation method comprises the steps of synthesizing a DNA (Deoxyribonucleic Acid) sequence coding the cecropin LL-37, and connecting the DNA sequence to a suitable expression vector for expression and purification, wherein the DNA sequence coding the cecropin LL-37 adopts Escherichia coli preferred codons, and the LL-37 expressed in an Escherichia coli fusion and expression system accounts for about 20% of the total protein of a thallus; and cutting off the purified fusion protein by using cyanogen bromide so as to obtain the target polypeptide LL-37, and obtaining LL-37 powder with the purity higher than 90% through HPLC (High-Performance Liquid Chromatography). The LL-37 of low concentration can play a significant bactericidal effect in the aspect of human preparations for external use, serves as a drug for external use and is applied to the treatment of diseases, such as skin infection. The methods have the advantages that the cecropin LL-37 is expressed and prepared in the form of fusion protein, the process is simple, and the yield of fermentation liquor per cubic liter can reach 20 mg, so that the methods have significance in industrial batch production.

The invention discloses a new utility of hepatitis B surface antigen-antibody compound in the hepatitis B vaccine non-respond or low-respond prevention agent, which is characterized by the following: purifying recombined HBsAG or inactivated serum HBsAG in the mammal cell, adopting hepatitis B with antibody serum or vaccine globulin as compound, improving anti-HBs ability for non-respond or low-respond mouse.

The invention discloses a method for preparing charge modified lactoferrin. The method comprises the following operation steps: dissolving 3-hydroxy-phtalic anhydride in dimethyl sulfoxide to prepare a saturated HP solution; dissolving lactoferrin in a 0.1M sodium phosphate solution to prepare a protein solution of 20mg / mL; uniformly mixing the saturated HP solution and the protein solution of 20mg / mL, regulating the pH to 10, standing at 25 DEG C for 1 hour, dialyzing by adopting PBS which has the pH of 7.4, and filtering and sterilizing with a 0.45mu m membrane to obtain the charge modified lactoferrin. The charge modified lactoferrin can be combined with carrageenan to prepare a composition medicine for preventing and treating infection of human papilloma virus of females and preventing and treating gynecological gram-negative bacterial infection of females.

The invention relates to a coxsackie A group 5 type (CV-A5) virus strain capable of effectively infecting adult young mice. The virus strain is CV-A5-M14-611, and has a complete amino acid sequence shown as SEQ ID NO. 1. The invention also relates to an attenuated strain CV-A5-M14-111 of the CV-A5 virus strain, and the attenuated strain has a complete amino acid sequence shown as SEQ ID NO. 3. Theinvention also relates to application of the virus strain in preparation of an animal model for evaluating vaccines and / or medicines for treating hand, foot and mouth disease (HFMD) and application of the virus strain in preparation of a preparation for evaluating a protection effect of the vaccines for treating the HFMD.