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Bacillus pyocyaneus vaccine and preparation method thereof

A technology for Pseudomonas aeruginosa and vaccines, applied in the directions of antibacterial drugs, bacterial antigen components, etc., can solve the problems that antibiotics are difficult to cure Pseudomonas aeruginosa infection, and Pseudomonas aeruginosa vaccines cannot meet clinical immunity, etc., and achieve the effect of enhancing immune effect.

Active Publication Date: 2009-04-15
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that current antibiotics are difficult to cure Pseudomonas aeruginosa infection, and the single-valence Pseudomonas aeruginosa vaccine cannot meet the needs of clinical immunity, and provide a Pseudomonas aeruginosa vaccine and its preparation method

Method used

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specific Embodiment approach 1

[0010] Specific embodiment one: the present embodiment Pseudomonas aeruginosa vaccine is made of Pseudomonas aeruginosa P placed in normal saline 1 endotoxin, Pseudomonas aeruginosa P 2 endotoxin, Pseudomonas aeruginosa P 6 endotoxin and Pseudomonas aeruginosa P 103 The toxoid composition of Pseudomonas aeruginosa vaccine; Pseudomonas aeruginosa P 1 The concentration of endotoxin in protein is 1 / 6~1 / 3mg / mL, Pseudomonas aeruginosa P 2The concentration of endotoxin in protein is 1 / 6~1 / 3mg / mL, Pseudomonas aeruginosa P 6 The concentration of endotoxin in protein is 1 / 6~1 / 3mg / mL, Pseudomonas aeruginosa P 103 The concentration of the toxoid is 0.5-1 mg / mL in terms of protein mass; among them, Pseudomonas aeruginosa P 1 Preserved in the China Medical Microbiology Culture Collection Management Center, the preservation number is 10115; Pseudomonas aeruginosa P 2 Preserved in the China Medical Microbiology Culture Collection Management Center, the preservation number is 10116; Pse...

specific Embodiment approach 2

[0012] Specific embodiment two: the present embodiment Pseudomonas aeruginosa vaccine is prepared according to the following steps: 1. Pseudomonas aeruginosa P 1 ,P 2 and P 6 Inoculate in beef extract peptone medium respectively and culture at 37-42°C for 18-24 hours, then wash Pseudomonas aeruginosa into shake flasks with endotoxin liquid synthesis medium and then incubate at 37-42°C, 175-200r / min Under shaking culture for 24-30 hours, then adjust the pH value of the endotoxin liquid synthesis medium to 6.5-8.5, then add toluene and put it in the environment at 37-42°C for 48-72 hours, and then use a 1000K-3000K filter plate for ultra- Filter and collect the filtrate; 2. Add ZnCl with a concentration of 30~50g / mL in the filtrate respectively 2 solution, then stirred, precipitated, centrifuged at 4°C and 4000r / min for 20min, discarded the supernatant, and 2 HPO 4 Add saturated Na to the precipitate at a volume ratio of solution to precipitate 5:1 2 HPO 4 Solution, then e...

specific Embodiment approach 3

[0022] Embodiment 3: The difference between this embodiment and Embodiment 2 is that the air temperature of the electric fan in Step 3 is 25-50°C. Other steps and parameters are the same as those in Embodiment 2.

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Abstract

The invention discloses a Pseudomonas aeruginosa vaccine and a preparation method thereof, which relate to a vaccine and a preparation method thereof. The invention solves the problems that the prior antibiotic is difficult to cure Pseudomonas aeruginosa infection, and a Pseudomonas aeruginosa vaccine with single valence can not satisfy the demand of clinical immunity. The vaccine consists of endotoxin of Pseudomonas aeruginosa P1, endotoxin of Pseudomonas aeruginosa P2, endotoxin of Pseudomonas aeruginosa P6 and toxoid of Pseudomonas aeruginosa P103 which are placed in physiological saline. The preparation method comprises the following steps: the endotoxins of Pseudomonas aeruginosaes are prepared; the toxoid of the Pseudomonas aeruginosa P103 is prepared; and the Pseudomonas aeruginosa vaccine can be obtained by mixing the endotoxins of the Pseudomonas aeruginosaes and the toxoid of the Pseudomonas aeruginosa P103. The Pseudomonas aeruginosa vaccine belongs to a polyvalent vaccine; the endotoxins of the Pseudomonas aeruginosa P1, the Pseudomonas aeruginosa P2 and the Pseudomonas aeruginosa P6 are adopted, so known type 11 Pseudomonas aeruginosa can be immunized; and the toxoid of the Pseudomonas aeruginosa P103 is added, so the immune effect of the vaccine to the Pseudomonas aeruginosaes is strengthened.

Description

technical field [0001] The invention relates to a vaccine and a preparation method thereof. Background technique [0002] Pseudomonas aeruginosa is an opportunistic pathogen with strong adaptability to the external environment and can survive for a long time in a humid environment. Pseudomonas aeruginosa exists in normal human skin, especially in moist parts (such as armpits, perineum, ear canal, respiratory tract and intestinal tract, etc.), and intact skin and mucous membranes can effectively resist Pseudomonas aeruginosa as a barrier However, the normal defense mechanism of the human body is reduced or damaged (such as skin and mucous membrane damage, agranulocytosis, hypoproteinemia, radiotherapy and chemotherapy of tumors, long-term application of hormones and antibiotics, burns, infant skin, umbilical cord, intestinal tract, Urinary system of the elderly) is prone to lead to Pseudomonas aeruginosa infection. [0003] According to reports, nosocomial infections caused...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/07A61P31/04
Inventor 孙建华曲晓军于冲夏海华刘宇峰王金英沙长青奚新伟于丽萍张云湖王雪娇
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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