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Universal virus sample preservation buffer solution and preparation method thereof

A general-purpose buffer technology, applied in biochemical equipment and methods, virus/bacteriophage, preservation of microorganisms, etc., to achieve the effect of maintaining the integrity of the virus and improving the integrity of the virus

Active Publication Date: 2020-03-31
阿吉安(福州)基因医学检验实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows us to store DNA from different viruses without losing their original structure by storing them separately instead on separate buffers that are compatible with each method being tested. It makes it easier to detect these agents through various techniques such as polymerase chain reaction (PCR) analysis, Western blotting, immunofluorography, etc., which helps improve our understanding about how diseases caused by genetic material may spread across multiple populations worldwide.

Problems solved by technology

This patents describes different ways to preserve and store cells containing DNA (deoxyribonucleic acid) with high resistance against harmful agents like bacteria or fungi. These techniques include extracting them out by physical means, culturing them at specific conditions, testing their ability to resist these attacks, and identifying new strain variants through genetic analysis. Additionally, certain chemical reagents have shown promise but they may affect cellular function due to changes caused during manufacture processes. To address this problem, an improved method called virus sampling and purification was developed based upon previous designs.

Method used

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  • Universal virus sample preservation buffer solution and preparation method thereof
  • Universal virus sample preservation buffer solution and preparation method thereof
  • Universal virus sample preservation buffer solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] S1) Accurately weigh 1100mL RN enzyme-free pure water, and divide the RN enzyme-free pure water into the first RN enzyme-free pure water and the second RN enzyme-free pure water, wherein the amount of the first RN enzyme-free pure water is RN enzyme-free 60% of the total consumption of pure water;

[0047]S2) Accurately weigh 1.44g magnesium chloride hexahydrate, 5.94g calcium chloride dihydrate and 3.06g imidazole; 4.5mL Triton X-100 and 0.9mL liquid biological preservative Proclin 300; and add the first RNase-free pure water In, the mixed solution is obtained;

[0048] S3) Fully stir the mixed solution for 25 minutes.

[0049] S4) Use 6N hydrochloric acid or 5N sodium hydroxide solution to adjust the pH value of the mixed solution to 6.8 under stirring;

[0050] S5) adding the second RNase-free pure water to the mixed solution treated in S4);

[0051] S6) Fully stir the solution prepared in S5) for 10 min.

[0052] S7) The universal virus sample storage buffer sol...

Embodiment 2

[0054] S1) Accurately weigh 1000mL RN enzyme-free pure water, and divide the RN enzyme-free pure water into the first RN enzyme-free pure water and the second RN enzyme-free pure water, wherein the amount of the first RN enzyme-free pure water is RN enzyme-free 70% of the total consumption of pure water;

[0055] S2) Accurately weigh 1.6g magnesium chloride hexahydrate, 6.6g calcium chloride dihydrate and 3.4g imidazole; 5.0mL Triton X-100 and 1.0mL liquid biological preservative Proclin 300; and add the first RNase-free pure water In, the mixed solution is obtained;

[0056] S3) Fully stir the mixed solution for 30 minutes.

[0057] S4) Use 6N hydrochloric acid or 5N sodium hydroxide solution to adjust the pH value of the mixed solution to 7.1 under stirring; the pH value can be set between 7.0 and 7.2 during specific implementation;

[0058] S5) adding the second RNase-free pure water to the mixed solution treated in S4);

[0059] S6) Fully stir the solution prepared in S...

Embodiment 3

[0062] S1) Accurately weigh 900mL RN enzyme-free pure water, and divide the RN enzyme-free pure water into the first RN enzyme-free pure water and the second RN enzyme-free pure water, wherein the amount of the first RN enzyme-free pure water is RN enzyme-free 80% of the total consumption of pure water;

[0063] S2) Accurately weigh 1.76g magnesium chloride hexahydrate, 7.26g calcium chloride dihydrate and 3.74g imidazole; 5.5mL Triton X-100 and 1.1mL liquid biological preservative Proclin 300; and add the first RNase-free pure water In, the mixed solution is obtained;

[0064] S3) Fully stir the mixed solution for 35 minutes.

[0065] S4) Use 6N hydrochloric acid or 5N sodium hydroxide solution to adjust the pH value of the mixed solution to 7.4 under stirring;

[0066] S5) adding the second RNase-free pure water to the mixed solution treated in S4);

[0067] S6) Fully stir the solution prepared in S5) for 20 min.

[0068] S7) The universal virus sample storage buffer sol...

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Abstract

The invention discloses a universal virus sample preservation buffer solution and a preparation method thereof in the technical field related to biological reagents. The universal virus sample preservation buffer solution comprises the following components: RN-free enzyme pure water, magnesium chloride hexahydrate, calcium chloride dihydrate, imidazole, triton X-100, a liquid biological preservative Proclin 300 and a pH value regulator. The preservation buffer solution being a universal virus sample preservation buffer solution not only is suitable for a PCR method or other molecular detectionmethods but also is suitable for detection of virus antigens or antibodies and has better capability of maintaining virus integrity; and the solution can be suitable for preservation of nucleic acids, antibody antigens or functional enzymes; preserved samples can be directly used for PCR, ElISA or colloidal gold, fluorescence and other detection methods.

Description

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Claims

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Application Information

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Owner 阿吉安(福州)基因医学检验实验室有限公司
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