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Antigen stabilizer and preparation method and application thereof

A technology of stabilizer and antigen, applied in the field of antigen stabilizer and its preparation, can solve the problems of reduced activity, shortened retention period, increased safety risks, etc., and achieves the effects of inhibiting oxidation, avoiding pollution, and reducing costs

Active Publication Date: 2017-03-08
BEIJING 3S CENTURY TECH CORP
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At very low concentrations, the stability of most antigens will be affected, and the activity will be reduced or the retention period will be shortened, which will affect the stability of the in vitro diagnostic kit.
It is difficult for a single buffer to stabilize the antigen at low concentrations, and some manufacturers add animal serum to keep the antigen stable, which increases safety risks and is not easy to maintain batch-to-batch consistency

Method used

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  • Antigen stabilizer and preparation method and application thereof
  • Antigen stabilizer and preparation method and application thereof
  • Antigen stabilizer and preparation method and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0031] Embodiment 1, preparation and application of antigen stabilizer

[0032] 1) Weigh 0.6 parts of tris-hydroxymethylaminomethane and dissolve it in 100 parts of purified water to form solution A, adjust the pH of solution A to 8.0 with hydrochloric acid, and obtain a Tris-HCl buffer solution with a molar concentration of 50 mM.

[0033] 2) Take 100 parts of the Tris-HCl buffer solution prepared in step 1), add 2.5 parts of BSA, 5 parts of glycerin, 5 parts of mannitol, 0.25 parts of β-mercaptoethanol, and 0.05 parts of Proclin-300, and stir evenly to obtain Antigen stabilizer.

[0034] The content of CRP in serum was detected by the double-antibody sandwich method, and the specific steps were as follows:

[0035] 1. Coating with CRP (C-reactive protein) antibody, 1mg / L, 100μl / well, overnight at 4°C (the coating solution is 0.159 parts of sodium carbonate and 0.293 parts of sodium bicarbonate dissolved in 100 parts of purified water);

[0036] 2. Dry the coating solution,...

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Abstract

The invention discloses an antigen stabilizer and a preparation method and application thereof. The antigen stabilizer comprises, by weight, 100 parts of Tris-HCl buffer solution, 1-5 parts of bovine serum albumin, 1-10 parts of glycerin, 1-10 parts of mannitol, 0.1-0.5 part of beta-mercaptoethanol and 0.01-1 part of Proclin-300. The antigen stabilizer can stabilize protein, inhibit protein oxidation, resist corrosion, avoid biological-source pollution and lower cost.

Description

technical field [0001] The invention relates to an antigen stabilizer and its preparation method and application, belonging to the field of biotechnology in vitro diagnostic reagents. Background technique [0002] Among in vitro diagnostic reagents, enzyme-linked immunodiagnostic reagents are recognized by researchers and medical staff because of their high sensitivity and accuracy, and are more and more widely used in clinical diagnosis, prompting the rapid development of enzyme-linked immunological diagnostic reagents. However, due to sensitivity requirements, antigens in ELISA reagents often need to be diluted to a very low concentration, such as less than 100 ng / ml. At very low concentrations, the stability of most antigens will be affected, and the activity will be reduced or the retention period will be shortened, which will affect the stability of the in vitro diagnostic kit. It is difficult for a single buffer to stabilize the antigen at low concentrations, and some...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N33/5306
Inventor 郑文果李国伟刘学仁
Owner BEIJING 3S CENTURY TECH CORP
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