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Preparation method for silver-containing carbon dot and application of silver-containing carbon dot to preparation of antibacterial agent

A silver-carbon and carbon dot technology, applied in the field of biomaterials, can solve the problems of poor biocompatibility of Cd quantum dots, and achieve the effects of good photostability, strong bacteriostatic function, and avoiding the harm of the natural environment.

Active Publication Date: 2018-04-20
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Cd-type quantum dots have poor biocompatibility. Due to their good biocompatibility, carbon dots have gradually shown the potential to replace conventional quantum dots in applications such as drug delivery, medical diagnosis, two-photon imaging, ion detection, and catalysis.
However, the application of carbon dots in antibacterial aspects is rarely involved in research groups.

Method used

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  • Preparation method for silver-containing carbon dot and application of silver-containing carbon dot to preparation of antibacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Take by weighing 0.1gPEI (molecular weight is 1800), 0.5g citric acid is in the round bottom flask of 50mL, then inject the silver nitrate of 50μL 0.1mol / L, add the ultrapure water of 30mL again and make it mix evenly, in After stirring for 20 min under the protection of nitrogen, a mixed solution was obtained.

[0046] (2) Transfer the mixed solution obtained in step (1) into a microwave reactor and heat it to 200° C., and react for 30 minutes to obtain the PEI-Ag carbon dot solution.

[0047] (3) Mix the PEI-Ag carbon dot solution obtained in step (2) with methanol at a volume ratio of 1:5, centrifuge at 5000 rpm for 30 minutes, remove the supernatant, and freeze-dry the resulting precipitate to obtain solid PEI-Ag carbon dots. point.

[0048] To dissolve in 0.1mol / L H 2 SO 4 Quinoline sulfate in solution (refractive index (η) 1.33) was used as reference (quantum yield=54%). By comparing the fluorescent area (E x =360nm) and the ratio of absorbance to calcula...

Embodiment 2

[0057] (1) Weigh 0.1g PEI (molecular weight is 600), 2g of citric acid in a 50mL round bottom flask, add 30mL of ultrapure water to mix evenly, stir for 20min under the protection of nitrogen, then pour into the round bottom flask 100μL of 0.1mol / L silver nitrate, and continue to stir for 20min.

[0058] (2) Transfer the mixed solution obtained in step (1) into a microwave reactor and heat it to 220° C., and react for 5 minutes to obtain the PEI-Ag carbon dot solution.

[0059] (3) Place the PEI-Ag carbon dot solution obtained in step (2) directly in a 3KD ultrafiltration centrifuge tube, centrifuge at 12,000 rpm for 10 minutes, remove the lower layer solution, and dry the upper layer solution with nitrogen to obtain solid PEI-Ag carbon dots. point. By measurement, the quantum yield of the PEI-Ag carbon dots synthesized in Example 2 is 55.5%.

[0060] (4) Inoculate Staphylococcus aureus in the MH broth liquid medium, place it in a constant temperature shaker, and cultivate i...

Embodiment 3

[0063] (1) Weigh 0.1g PEI (molecular weight 10000), 1g citric acid in a 50mL round bottom flask, add 30mL ultrapure water to the round bottom flask to mix evenly, and stir for 20min under the protection of argon.

[0064] (2) Transfer the mixed solution obtained in step (1) into an autoclave and heat it to 220° C., and react for 30 minutes to obtain the PEI carbon dot solution.

[0065] (3) Inject 30 μL of 0.1mol / L silver nitrate into the PEI carbon dot solution, and stir for 20 min under the protection of argon to obtain the PEI-Ag carbon dot solution.

[0066] (3) Mix the PEI-Ag carbon dot solution obtained in step (3) with acetonitrile in a volume ratio of 1:2, centrifuge at 15000rpm for 5min, remove the supernatant, and dry the resulting precipitate with argon to obtain solid PEI-Ag Ag carbon dots. By measurement, the quantum yield of the PEI-Ag carbon dots synthesized in Example 3 was 53.3%.

[0067] (4) Inoculate the Candida albicans strains in the improved Martin liqu...

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Abstract

The invention discloses a preparation method for a silver-containing carbon dot and application of the silver-containing carbon dot to preparation of an antibacterial agent. The preparation method comprises the following steps: with polyethyleneimine (PEI), citric acid and silver nitrate as raw materials, synthesizing a silver-doped functionalized carbon dot solution (a PEI-Ag carbon dot solution)by using a hydrothermal process or microwave process; and then carrying out centrifugation and drying so as to obtain the solid PEI-Ag carbon dot. The preparation method provided by the invention isenvironment-friendly in preparation and uses cheap and easily available raw materials; and the prepared PEI-Ag carbon dot has good biocompatibility and high quantum yield, can be individually used orcooperatively used with antibacterial agents like tetracycline, gentamycin and cefoxitin, and show inhibitory effect on Staphylococcus aureus, Escherichia coli, Candida albicans and the like.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a method for preparing functionalized silver-containing carbon dots and the application of the carbon dots in antibacterial aspects. Background technique [0002] Infectious diseases caused by pathogenic microorganisms hinder the normal growth and reproduction of animals and plants, resulting in crop yield reduction, animal disease, and endanger human health. With the continuous emergence of drug-resistant bacteria, traditional antibacterial drugs are facing more and more severe challenges in hygiene prevention and disease treatment, and people's demand for high-efficiency antibacterial materials is becoming increasingly urgent. [0003] Due to the broad-spectrum bactericidal properties and excellent antibacterial properties of nanomaterials, it is difficult for pathogenic microorganisms to develop drug resistance to nanomaterials. In recent years, nanomaterials have been wi...

Claims

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Application Information

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IPC IPC(8): A01N59/16A01N37/44A01N43/16A01N43/90A01N43/50A01N43/60A01N43/653A01N43/22A01P1/00A01P3/00
CPCA01N37/44A01N43/16A01N43/22A01N43/50A01N43/60A01N43/653A01N43/90A01N59/16
Inventor 赵丹韦春锦郝健
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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