Culture and preparation method for pyricularia oryza conidia

Abstract

The invention discloses a culture and preparation method for pyricularia oryza conidia. According to the method, the multi-starting-point culture technology and the aerial mycelium conidium production technology are utilized for fast culturing and preparing the pyricularia oryza conidia. The method comprises the following implementation steps that 1, a conidium production culture medium is prepared; 2, a multi-starting-point transplanting thallus is prepared; 3, the multi-starting-point transplanting thallus is planted in a culture plate; 4, the conidia are prepared and cultured; 5, the conidia are eluted and separated. The method has the advantages that fastness and high efficiency are achieved, and conidia of a new generation can reach the ideal state of a high sporulation quantity after five days of culture usually; sterile operation can be kept in the whole culture process, and the contamination probability of a finished conidium solution is greatly reduced; the operation technology is simple, and the result sporulation quantity is large.

Description

technical field [0001] The present invention relates to phytopathological techniques and microbiological techniques. Specifically, it is a method for cultivating and preparing conidia of rice blast fungus. Background technique [0002] In addition to the basic role of reproduction and expansion of the population of plant pathogenic fungi, the propagules of plant pathogenic fungi have the function of interacting with the host and initiating the infection of the host. Therefore, in the research process of most plant diseases, it is necessary to Using the propagules of pathogenic bacteria as test materials, it is obvious that an efficient and convenient method for culturing and preparing propagule materials will be very beneficial to the efficient development of research work. Rice blast is a major disease of rice production in my country. The propagules of the pathogen (Magnaporthe grisea) include two types: sexual ascospores and asexual conidia. At present, it is very diffi...

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Problems solved by technology

However, this method is still suspected of taking too long time; and there is still a high probability of bacterial contamination in the long-term plate culture; the technical operation of scraping off the aerial hyphae in the middle of the culture, and the need to provide technical support such as lighting, Practical application is still inconvenient

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