283 results about "Genetic transfection" patented technology

The invention discloses a gene vector system of a redox sensitive shielding system having targeting function, its preparation and application in the field of gene therapy. The gene vector system disclosed herein comprises a redox sensitive shielding system having targeting function, a cationic high-molecular material and plasmid DNA; wherein the cationic high-molecular material and plasmid DNA form compound particles, the redox sensitive shielding system having targeting function shields the compound surface by electrostatic action, thus the toxicity of the vector can be reduced, the loaded genetic material is transferred into the cell successfully, the expression of the genetic material is realized, the transfection process is completed, the targeting of gene transfection can be raised, and simultaneously the gene transfection efficiency is raised.

The invention discloses an oligonucleotide aptamer for prostate cancer targeting gene, a delivery carrier, a delivery system and a preparation method thereof. The delivery carrier is made of polycationic polymer material, hydrophilic polymer and Based on the oligonucleotide aptamer, the three are covalently linked, wherein the molar ratio of the hydrophilic polymer to the polycation polymer is 1-25:1, and the oligonucleotide aptamer The molar ratio of the body to the polycation polymer material is 1-5:1; the delivery system is a nanoscale gene delivery system formed by delivery carriers and miRNA, siRNA or plasmid DNA. The delivery carrier and the delivery system of the present invention can effectively improve the gene transfection efficiency and expression efficiency on prostate cancer cells expressing prostate specific membrane antigen, compared with the gene delivery system constructed by targeting the head group used in the prior art , which has the advantages of stronger stability and significantly improved targeting effect.

The invention discloses a genetic vector system of a nanoparticle with multiple oxidation-reduction stimulus response as well as a preparation method and application of the genetic vector system. The genetic vector system is composed of a shielding system, a cation material and foreign plasmid DNA (deoxyribonucleic acid); and the shielding system and the cation material both have oxidation-reduction stimulus response, so that a ternary complex nanoparticle with multiple oxidation-reduction stimulus response is formed. The genetic vector of the ternary complex nanoparticle is low in cytotoxicity, and capable of well compressing and compounding the plasmid DNA under physiological conditions, successfully transferring a supported genetic substance into a cell and entering the cell; and the reduction environment in the cell can be more rapidly broken to release the supported gene, so that the expression of gene substances is realized, the transfection process is finished, and the gene transfection efficiency can be remarkably increased.

The invention discloses cationic lipid containing peptide dendrimer, a transgenic carrier, and a preparation method and application of the transgenic carrier. The transgenic carrier has low cell toxicity, can effectively retard and coat pEGFP-N1 plasmid or pGL3 plasmid, and is high in coating capacity. As the average particle size of a compound of the transgenic carrier with the pEGFP-N1 plasmid or pGL3 plasmid ranges from 80 nm to 200 nm, and the Zeta potential is within the range of 5-50mV, the transgenic carrier is quite suitable for gene transfection. When the compound formed of the transgenic carrier and the pEGFP-N1 plasmid or pGL3 plasmid is used for gene transfection of cells HepG2, MCF7 and B16F10, good transfection capacity is achieved, the transfection capacity is higher than that of the commercial product PE125K on the condition of transfection optimization, and the transfection advantage is much superior on the condition with serum existence.

The invention discloses a preparation method for a replication and transcription activator (Rta) protein and the application of the Rta protein to a nasopharynx cancer detection reagent and relates to a medical diagnosis reagent. The preparation method disclosed by the invention comprises the following steps of: 1, constructing a recombinant expression vector by taking a BRLF1 full-length gene as an exogenous gene; 2, transfecting: transfecting the recombinant expression vector into an eukaryotic expression system to obtain a positive transfected cell; and 3, expressing and purifying: culturing the positive transfected cell so as to enable the positive transfected cell to express an interest protein, and separating and purifying the interest protein, wherein the eukaryotic expression system refers to a Chinese hamster ovary (CHO) cell. The Rta protein prepared by the method disclosed by the invention is used for detecting nasopharynx cancer; the sensitivity of the Rta protein is 96 percent (288 / 300), and the specificity of the Rta protein is 96.7 percent (290 / 300). The sensitivity and the specificity are superior to those of antigens respectively prepared by a prokaryotic expression system and a pichia expression system, and the sensitivity and the specificity on clinical early diagnosis on the nasopharynx cancer are greatly improved.

The invention relates to a ZnO quantum dot vector / DNA composite-containing collagen-based composite cornea substitute, and a preparation method and application thereof. The ZnO quantum dot vector / DNA composite-containing collagen-based composite cornea substitute is prepared by absorbing ZnO quantum dot vectors / DNA composites by using a collagen / MPDSAH IPN cornea substitute, wherein the weight ratio of the cornea substitute to the ZnO quantum dot vectors / DNA composites is 425:1; and the weight ratio of ZnO quantum dot vectors to DNA is 25:1. The ZnO quantum dot vector / DNA composite-containing collagen-based composite cornea substitute has high biocompatibility, can induce and promote the regeneration of a cornea and be biologically decomposed along with the regeneration of the cornea, hasmechanical properties and optical properties the same as those of a human cornea, remarkably improves the stability and mechanical strength of the cornea substitute in collagenase due to the introduction of an MPDSAH polymer network, can effectively compositely compress the DNA, successfully induce the DNA into cells, successfully express the DNA, trace the position of the DNA / vector at any time and determine the intra-cellular distribution of the DNA / vector in a transgenosis process due to ZnO quantum dots, and effectively combines gene transfection, tissue engineering and fluorescent tracing so as to achieve simple manufacturing method, and easy processing and long-term storage and transport.

The invention relates to a multifunctional immune killing transgenic cell and in particular relates to a multifunctional immune killing transgenic cell which has cell killing toxicity and expressing an antibody containing a human constant region. The gene of the antibody containing the human constant region is transfected into a cell with cell killing toxicity through coding, and is expressed in the cell at a high level so as to realize the double effects of cell immunity and antibody and inhibit multiplication of tumor and virus. The invention also relates to a preparation method and use of the multifunctional immune killing transgenic cell.