333 results about "Snake venom" patented technology

The invention relates to the inhibition of blood coagulation, especially during organ rejection, and in particular the inhibition of delayed vascular rejection. The invention provides anticoagulant proteins which are anchored to cell membranes. The anticoagulant function preferably provided by heparin, antithrombin, hirudin, TFPI, tick anticoagulant peptide, or a snake venom factor. These anticoagulant proteins are preferably prevented from being constitutively expressed at the cell surface. In particular, expression at the cell surface is regulated according to cell activation, for instance by targeting the protein to a suitable secretory granule. Expression of these proteins renders cells, tissues and organs less vulnerable to rejection after transplantation (e.g. after xenotransplantation).

The invention provides sequences of a snake venom cathelicidin antibacterial peptide consisting of non-natural fully D-type amino acid, which cannot be easily degraded by protease, and a derivative thereof and a preparation method thereof, and also provides application of the snake venom cathelicidin antibacterial peptide consisting of the non-natural fully D-type amino acid and the derivative thereof. The snake venom cathelicidin antibacterial peptide consisting of the non-natural fully D-type amino acid and the derivative thereof have an obvious effect of suppressing growth of bacteria and fungus, in particular to an excellent effect of suppressing clinical drug-resistant bacteria, have the beneficial characteristics of simple structure, convenience in artificial synthesis and wide antibacterial spectrum system, have the obvious characteristics of capability of resisting degradation of various protease, long antibacterial activity retention time and no drug residue, and can be applied to prevention and treatment of human or animal infected diseases, clinical artificial biomaterials, food preservative and fruit and vegetable refreshment, feed additives and daily chemical supplies.

The present invention is one kind snake venom cytozyme hemostatic containing blood coagulating factor, activator RVV-X and synergist snake venom thrombase. Preferably, the snake venom cytozyme hemostatic contains also serum albumin, for example human serum albumin. In addition, the present invention also discloses the preparation process and hemostatic use of high purity RVV-X.

The invention relates to a method for extracting cobratide, a medicine used to treat chronic pain, the cobratide extracted by the method and a preparation containing the cobratide. The method for extracting the cobratide comprises the following steps: firstly, a stock solution of snake poison of a cobra is pretreated with ammonium sulphate to remove partial other compositions in the snake poison,is subjected to desalination and concentration through ultrafiltration, dialysis or gel chromatography, and after the concentration, is purified by a cation column, and finally is subject to freeze-drying preservation after the concentration. The cobratide preparation comprises an enteric preparation, a rectal administration preparation, a colon-specific administration preparation and so on. Withcobratide extraction and purification processes provided by the method, the purity of the extracted cobratide is more than 95 percent, and the yield reaches 70 percent; and the method is simple and reliable, and saves cost and time.

The invention discloses a method for refining a polyvalent ASV (anti-snake venom) lyophilized serum, pertaining to an immune serum containing antibody which aims at least two kinds of antigens. The serum takes the venom of Ancistrodon acutus, Agkistrodon halys, Pit-vipers, bamboo snakes, adders and the like as the immune antigen which is inoculated into the bodies of animals after degerming and dialysis, and then titer immune serum after purification is used for collecting the antibody which is then desalted and lyophilized; mice are used for evaluating the LD50 of the virulence of the venom and the titer of the ASV serum; the antibody inoculated into the bodies of the animals is one of the venom, toxoid or snake venom or the composition thereof, and glutaraldehyde or formaldehyde is used for toxin detoxication; before immunity, anti-tetanic toxoid condensed antibody for human beings is used for carrying out minim gradual immunization on the animals, or an Emuade adjuvant is used for immunization purpose. The polyvalent ASV lyophilized serum complies with the biological product standards upon the tests and inspections of purity and specific activity, safety, pyrogen, hypersusceptibility, dissolution and the like; moreover, the serum can cure sufferers who are bitten by vipers with the advantages of high purity, low foreign protein, easy preservation and quick dissolving capacity.

Aliquots of extracts from ethnopharmacological plants that have activity against the effects of sarafotoxins present in snake venom are isolated and identified as antagonists of endothelin using a fluorescence-based assay. A process is provided for the identification of an antagonist of an endothelin selected from the group consisting of endothelin-1, endothelin-2, endothelin-3 and mixtures thereof. The process comprises extraction of ethnopharmacological plants with a solvent followed by evaporation of the solvent to form an aliquot containing at least one component of the extract, optionally purifying and isolating one or more component by chromatography, and subjecting the aliquot or purified component to a competitive fluorescent binding assay using biotinylated endothelin-1, wherein the plants having activity against the effects of one or more sarafotoxins present in snake venom.

The invention discloses a synthesis method of a liquid-phase segment of a snake-venom-like tripeptide. The synthesis method comprises the following steps of adopting Fmoc-Pro-OH, H-DAB(Boc)-OH and benzylamine with low cost as raw materials to synthesize a protective segment H-Pro-DAB(Boc)-NH-Bzl firstly, then using Boc-beta-Ala-OH and H-Pro-DAB(Boc)-NH-Bzl to synthesize Boc-beta-Ala-Pro-DAB(Boc)-NH-Bzl, and after trifluoroacetic acid is adopted to carry out protecting-group removal on the Boc-beta-Ala-Pro-DAB(Boc)-NH-Bzl, separating, purifying and freeze-drying to obtain H-beta-Ala-Pro-DAB-NH-Bzl with the purity being greater than 95%, namely the snake-venom-like tripeptide. The synthesis method disclosed by the invention is simple and easy to implement and is low in cost; a synthesis method of the liquid-phase segment, with simple process and lower cost, is provided for synthesizing the snake-venom-like tripeptide.

The invention relates to phospholipase(s), isoforms, derivatives, mutants and / or fragments thereof, for the preparation of a medicament for the treatment and / or prevention of ischemia. Preferred is the use of secretory phospholipase, particularly phospholipase A2, and even more particularly phospholipase A2 derived from the snake venom of Naja sputatrix

The present invention is chicken yoke antibody (IgY) resisting Bengalese cobra venom and its preparation process. The preparation process includes water dilution to extract coarse IgY from chicken yoke, salting out IgY with ammonium sulfate, dissolving the precipitate in PBS, ultrafiltering to desalt, concentrating, anion exchanging chromatographic separation for further purifying IgY, ultrafiltering to desalt, concentrating, and freeze drying. The IgY exhibits single tape of molecular weight 200 KD in non-reductant SDS-PAGE and two tapes of molecular weight 65 KD and 35 KD separately in reductant SDS-PAGE, and has at least 13 dyeing strips of molecular weight 100-0.7 KD on PVDF film for Western Blot detection. The chicken yoke antibody (IgY) resisting Bengalese cobra venom may be used in preparing medicine and health food for preventing and / or treating snake bite, and in preparing reagent for detecting relevant snake venom.

The invention discloses a purification method, an extract and a preparation of cobra venom neurotoxin. The extract of the cobra venom neurotoxin is prepared from cobra crude venom through extraction and purification. A filter membrane filtering method is firstly adopted for removing germs, particles and macromolecular sensitizers in solution, the time and the cost are saved for the subsequent processes, and the safety of products is enhanced; an ion exchange method is adopted for eluting and separating the neurotoxin, and the separation of neurotoxin protein is improved; and finally, acetone is adopted for directly precipitating the cobra venom neurotoxin, and impurities in final products are removed. Compared with the prior art, the extract and the preparation obtained in the invention have the advantages that the product yield, the content and the purity are improved, the adverse reaction of the medicine is effectively reduced, the curative effect is improved, and the quality of products is ensured.

The invention discloses a method for detecting and identifying the variety of snake venom by utilizing an adaptor technology, belonging to the field of biomedicine verification and relating to a method for detecting snake venom by utilizing the adaptor technology. Specific to the problems complex compositions of snake venom and poor specificity and sensitivity of the traditional snake bite detection method, the invention adopts a depletion SELEX (Systematic Evolution of Ligands by Exponential Enrichment) technology, and an adaptor with high-degree snake venom species specificity is obtained through screening and is converted into a report adaptor so as to establish a novel method for quickly detecting snake venom.

The present invention relates to a polypeptide skin beautifying youth essence. The polypeptide skin beautifying youth essence comprises the following components and proportions: 70g of water, 0.02g ofdisodium EDTA, 5g of glycerol, 3g of propylene glycol, 0.08g of xanthan gum, 0.2g of allantoin, 1g of hyaluronic acid, 0.1g of nipagin methyl ester, 0.5g of D-panthenol, 0.2g of oat B-glucan, 0.1g ofHPS, 0.02g of incense, 0.1g of MTI, 1g of centella asiatica extract, 0.1g of snake venom peptide, 0.1g of hexapeptide-8, 0.1g of decapeptide-12, 0.3g of oligopeptide-3, 0.1g of rice peptide, and 1g of purslane herb extract. The polypeptide skin beautifying youth essence provided by the invention can slow down the aging process of skin, help moisturize the skin, promote collagen synthesis and skinregeneration, improve the skin texture and appearance, make the skin elastic and reduce wrinkles; and at the same time, the water retention performance of the skin is increased, so that the polypeptide skin beautifying youth essence plays the role of preserving moisture and moisturizing the skin and protects the skin from damages.

The present invention discloses an anti-tumour snake venom cytotoxin and a production technique thereof, belonging to the fields of biochemistry and biological medicines. The snake venom cytotoxin of the present invention is derived from the prior lyophilized cobra venom powder. In the production technique of the present invention, the lyophilized cobra venom powder is resolved in the known buffer solution, and then on a SP-Sepharose H.P cation exchange column (5.0cm multipled by 29cm), gradiently eluted stage by stage by phosphate buffer and NaC1 at 10ml per tube and the flowing speed of 18ml per minute, a KTA Explorer protein purification system is used to monitor and collect eluting peaks, XII and XIII peaks with cytotoxic effects are collected, and via Source 30RPC reversed phase chromatography, cytotoxin XII and cytotoxin XIII, the molecular weight of which is about 6000 daltons to 7000 daltons and the isoelectric point of which is larger than 10, are respectively obtained. Compared with the purification step of the prior art, the production technique for producing the pure anti-tumour snake venom cytotoxin product without PLA2 has good rapid repeatability and is suitable for expanded production.

Purified multivalent and lyophilized antivenin and a use method belong to antibody immunity serums that aim at more than two antigens. The invention aims at preparing the multivalent antivenin by a plurality of lyophilized antivenins and the use method. The invention is characterized in that: the antigen-binding fragment F(ab)2 has 60 to 80 percent of lyophilized antiserum, the unit is mg per person, 342.5 to 1035 of vipers, 1177 to 3182 of agkistrodon acutus, 456 to 666 of adders, 1419 to 2128 of king cobras, 220 to 331 of coral snakes, 880 to 1320 of gold banded kraits, 3188 to 4782 of cobras are combined; the optimized combination is as follows: 635 of vipers, 1959 of agkistrodon acutus, 561 of adders, 1774 of king cobras, 276 of coral snakes, 1100 of gold banded kraits and 3985 of cobras. Blood circulation snake venom antiserum and nerves snake venom antiserum are respectively filled and measured. The use method is as follows: the antivenin that is diluted by sodium chloride and an anti-allergic agent, such as chlorphenamine maleate, are injected into muscle or a vein. The antivenin is prepared by only seven antivenins, and can effectively and in time treat injuries caused by various poisonous snakes in our country when the antiserum is directly injected in condition of the undetermined variety of poisonous snakes, and the invention reaches the advanced world level of the antivenin.

The invention discloses a snake venom metal protease inhibitor BJ46a capable of resisting attack and transference of tumor and application thereof, and belongs to the field of medical organism. In the invention, according to the BJ46a gene sequence (AF294836) in GenBank, the BJ46a full gene is designed and synthesized. An expression vector cloned to baculovirus can generate the recombined BJ46a protein capable of inhibiting the activity of the substrate metal protease in Sf9 insect cells, and in vivo and in vitro experiments show that the BJ46a protein has the functions of resisting the attack and transference of melanoma cells B16. The inhibitor adopts the genetic transfection technique, and can establish B16 / pcDNA3.1HisC-BJ46a cell strains with stable transfection, and the in vivo and in vitro experiments prove that the BJ46a can inhibit the attack and transference of B16 cells at the gene level. The inhibitor is applied to preventing and treating the attack and transference of tumor, and has great application prospect.

The present invention provides a wheat germ cell-free protein synthesis system for high level expression of recombination snake venom kininogenase. According to the system, snake venom kininogenase gene of agkistrodon halys pallas is cloned through reverse transcription, wheat germ cell-free expression plasmid pCS<2+> / VK is constructed, a wheat germ extract is prepared, an in vitro transcription and translation system is established, the snake venom kininogenase is expressed and the system is optimized. The N terminal of the exogenous protein expressed by the expression system has the His protein tag, and a Ni column elution method is adopted to carry out purification, such that the subsequent purification of the recombinant protein is substantially simplified, the purification efficiency is high, and the cost is low. The exogenous protein further has biological activity, and provides the foundation for the next clinical study.

The invention provides polypeptide anti-wrinkle and anti-aging essence. The polypeptide anti-wrinkle and anti-aging essence is prepared from the following components in percentage by mass: 0.5 to 2 percent of carnosine; 0.5 to 5 percent of snake venom-like tripeptide; 0.5 to 4 percent of acetyl hexapeptide-8; 0.1 to 2 percent of oligopeptide-1; the mask is prepared from, by weight, 1%-4% of dipalmitoyl hydroxyproline, 80.5%-88% of deionized water, 0.5%-3% of betaine, 0.1%-0.5% of p-hydroxyacetophenone, 0.1%-0.2% of xanthan gum, 0.2%-4% of butanediol, 0.1%-1% of sodium hyaluronate, 0.5%-2% of oat beta-glucan and 0.1%-2% of hexanediol. The polypeptide-based anti-wrinkle and anti-aging essence disclosed by the invention can make skin, especially eye skin, moist and moisturize for a long time,comprehensively replenish cell nutrition and slow down generation of true wrinkles of eyes.

The preparation method of five-venom chicken for preventing and curing cancers includes: respectively extracting snake venom, scorpion venom, centipede venom, house lizard venom and toad venom, mixing them and injecting them into poisonous snake body, using said poisonous snake as carrier of venom, feeding it into the crop of live cock, after the poisonous snake is digested and absorbed by said live cock and the venom is bio-converted, the cock can be choked to death, processed and eaten by cancer's pateint. Said invention can raise immunity of patient, can be used for curing cancer of lung, carcinoma of esophagus, carcinoma of stomach and intestinal cancer, etc..

The invention relates to chito-oligosaccharide, in particular to an N-benzene acryloyl chito-oligosaccharide and the preparation thereof; wherein, the chito-oligosaccharide is provided with mixed components with the average molecular weight from 800 to 6000 and the degree of deacetylation from 80 to 100 percent; the degree of substitution of the benzene acryloyl is from 30 to 100 percent; the general structure formula of the components is provided as follows, the chito-oligosaccharide has the effects of lowering the blood fat and blood glucose, enhancing the immune function, inhibiting the tumor and fighting against the fatigue and delaying the aging as well as alleviating the analgesia, diminishing the inflammation and the effects of anti liver virus, anti snake venom, etc., and can be served as the important raw material for certain medicines, health food and cosmetics.

The invention discloses a rapid snake wound identification method using an enzyme mark strip as a carrier, which includes that an enzyme mark hole is coated by a snake venom species specific antibody, blood serum collected from a patient with a snake wound is dropped in the hole, and snake wound types can be judged in a short time. Screening of various specific antibodies and preparation of the enzyme mark strip and auxiliary reagents in the testing process are low in cost; a testing process is simple without the help of instruments, and testers do not need abundant clinical diagnosis experience; testing time is short, and the time from taking out the enzyme mark strip under low temperature to result developing is about 40-45 minutes; accuracy is high, an AB-double-antibody sandwich method can effectively guarantee combination of snake venom and specific antibodies, biotin-avidin amplifies signals effectively, and developing results can be observed directly through naked eyes.

The invention discloses a method for refining a polyvalent ASV (anti-snake venom) lyophilized serum, pertaining to an immune serum containing antibody which aims at least two kinds of antigens. The serum takes the venom of Ancistrodon acutus, Agkistrodon halys, Pit-vipers, bamboo snakes, adders and the like as the immune antigen which is inoculated into the bodies of animals after degerming and dialysis, and then titer immune serum after purification is used for collecting the antibody which is then desalted and lyophilized; mice are used for evaluating the LD50 of the virulence of the venom and the titer of the ASV serum; the antibody inoculated into the bodies of the animals is one of the venom, toxoid or snake venom or the composition thereof, and glutaraldehyde or formaldehyde is used for toxin detoxication; before immunity, anti-tetanic toxoid condensed antibody for human beings is used for carrying out minim gradual immunization on the animals, or an Emuade adjuvant is used for immunization purpose. The polyvalent ASV lyophilized serum complies with the biological product standards upon the tests and inspections of purity and specific activity, safety, pyrogen, hypersusceptibility, dissolution and the like; moreover, the serum can cure sufferers who are bitten by vipers with the advantages of high purity, low foreign protein, easy preservation and quick dissolving capacity.

The invention discloses an application of physically-modified Chinese cobra venom in preparation of medicaments for treating acute and chronic nephrosis. The physically-modified Chinese cobra venom is obtained by dissolving Chinese cobra venom with deionized water, heating for denaturation and then performing renaturation; the heating temperature is controlled to be 50-100 DEG C, and the heating time is controlled to be 1 min-300 min. The invention finds that the heated and denaturated Chinese cobra venom can effectively reduce urine protein in acute and chronic nephrosis, reduce serum creatinine, and urea nitrogen, improve the pathological damage degree of the kidney, and recover kidney functions.

A device for treating a snake bite wound that utilizes a hypodermic tube with a curvature that is similar to a typical snake fang whereby the hypodermic tube on one end comprises a smooth bulbous tip with at least one opening and a vacuum source is attached to the other end of the hypodermic tube. The tip is inserted into a snake bite wound, and due to the curved shape of the hypodermic tube, the tip and the tube then follow the curved shape of the snake bite wound without damaging any of the tissue surrounding the snake bite canal. The vacuum source then extracts any venom that is present from the wound. The device also enables medicine to be injected into the wounded area to treat the wound.

The invention discloses a method for catalytic synthesis of a dicoumarin compound through lipases. The method comprises the step: 4-hydroxycoumarin is adopted as a substrate, and subjected to a Knoevenagel-Michael cascade reaction through lipase catalyzing to generate the dicoumarin compound with a compound in the formula II. The dicoumarin compound can be used for treating thrombus, resisting HIV, and treating asthma, skin cancer and snake venom, and can further be used for antimicrobial treatment of certain staphylococcus aureus and staphylococcus epidermidis and preparing of analytical reagents; and according to the method, reacting conditions are mild, aftertreatment is easy, the substrate is widespread, a catalyst can be recycled, and the method is an economical and environment-friendly new method for synthesizing the dicoumarin compound.

The invention relates to the inhibition of blood coagulation, especially during organ rejection, and in particular the inhibition of delayed vascular rejection. The invention provides anticoagulant proteins which are anchored to cell membranes. The anticoagulant function preferably provided by heparin, antithrombin, hirudin, TFPI, tick anticoagulant peptide, or a snake venom factor. These anticoagulant proteins are preferably prevented from being constitutively expressed at the cell surface. In particular, expression at the cell surface is regulated according to cell activation, for instance by targeting the protein to a suitable secretory granule. Expression of these proteins renders cells, tissues and organs less vulnerable to rejection after transplantation (e.g. after xenotransplantation).

Purified multivalent and lyophilized antivenin and a use method belong to antibody immunity serums that aim at more than two antigens. The invention aims at preparing the multivalent antivenin by a plurality of lyophilized antivenins and the use method. The invention is characterized in that: the antigen-binding fragment F(ab)2 has 60 to 80 percent of lyophilized antiserum, the unit is mg per person, 342.5 to 1035 of vipers, 1177 to 3182 of agkistrodon acutus, 456 to 666 of adders, 1419 to 2128 of king cobras, 220 to 331 of coral snakes, 880 to 1320 of gold banded kraits, 3188 to 4782 of cobras are combined; the optimized combination is as follows: 635 of vipers, 1959 of agkistrodon acutus, 561 of adders, 1774 of king cobras, 276 of coral snakes, 1100 of gold banded kraits and 3985 of cobras. Blood circulation snake venom antiserum and nerves snake venom antiserum are respectively filled and measured. The use method is as follows: the antivenin that is diluted by sodium chloride and an anti-allergic agent, such as chlorphenamine maleate, are injected into muscle or a vein. The antivenin is prepared by only seven antivenins, and can effectively and in time treat injuries caused by various poisonous snakes in our country when the antiserum is directly injected in condition of the undetermined variety of poisonous snakes, and the invention reaches the advanced world level of the antivenin.

The invention discloses a method of extracting defibrase from snake poison and preparing defibrase injection preparation, and the process of extracting defibrase includes the steps: preparing monoclonal antibody; preparing crude defibrase; preparing antibody affinity chromatography column; preparing defibrase; and the process of preparing the defibrase injection preparation includes the steps: taking and adding purified defibrase to a proper amount of injection water for dissolving so as to obtain a solution, adding 5-20g NaCl stabilizer to every 1000ml solution, making split charging and obtaining the defibrase injection preparation.

The invention belongs to the technical field of determination of biological immune methods, and particularly relates to a detection card for identifying bungarotoxin by using colloidal gold immunochromatography, and a preparation method and application thereof. The detection card is composed of a PVC backboard, a nitrocellulose membrane (NC membrane), a colloidal gold pad, a sample pad and water absorption filter paper. The sample pad is made of glass fibers. A colloidal gold labeled rabbit anti-beta-BGT polyclonal antibody is sprayed on the colloidal gold pad. The C line of the nitrocellulose membrane is coated with a goat anti-rabbit IgG polyclonal antibody, and the T line of the nitrocellulose membrane is coated with a rabbit anti-beta-BGT polyclonal antibody. The preparation method is simple, the specificity is high, the stability is good, the detection method is simple, carrying is convenient, bungarotoxin can be rapidly detected, whether a person is bitten by bungarus multicinctus or not can be judged within 5-10 minutes, a diagnostic tool for judging venomous snake bites can be provided for snake bite treatment, more treatment time can be gained for patients, and the detection card can assist in the clinical anti-snake venom serum dosage, and is economical and practical.

The invention discloses application of cytotoxin (CTX1) from snake venom to preparation of a medicament for rehabilitating. Proved by the experiment, the CTX1 can better eliminate the behavioral sensitization of addict rats by morphine, eliminate the medicament thirst behavior and the abstinence syndrome of rats and has favorable rehabilitating effect. In addition, the invention can improve the function state, the diet and the activities of the addict rats and remarkably increase the weight, thereby recovering normal physiological activity.