Refined polyvalent anti-snake poison lyophilized blood serum and using method

Abstract

Purified multivalent and lyophilized antivenin and a use method belong to antibody immunity serums that aim at more than two antigens. The invention aims at preparing the multivalent antivenin by a plurality of lyophilized antivenins and the use method. The invention is characterized in that: the antigen-binding fragment F(ab)2 has 60 to 80 percent of lyophilized antiserum, the unit is mg per person, 342.5 to 1035 of vipers, 1177 to 3182 of agkistrodon acutus, 456 to 666 of adders, 1419 to 2128 of king cobras, 220 to 331 of coral snakes, 880 to 1320 of gold banded kraits, 3188 to 4782 of cobras are combined; the optimized combination is as follows: 635 of vipers, 1959 of agkistrodon acutus, 561 of adders, 1774 of king cobras, 276 of coral snakes, 1100 of gold banded kraits and 3985 of cobras. Blood circulation snake venom antiserum and nerves snake venom antiserum are respectively filled and measured. The use method is as follows: the antivenin that is diluted by sodium chloride and an anti-allergic agent, such as chlorphenamine maleate, are injected into muscle or a vein. The antivenin is prepared by only seven antivenins, and can effectively and in time treat injuries caused by various poisonous snakes in our country when the antiserum is directly injected in condition of the undetermined variety of poisonous snakes, and the invention reaches the advanced world level of the antivenin.

Description

Technical field [0001] The present invention belongs to immune serum containing antibodies, especially immune serum containing antibodies against two or more antigens. Background technique [0002] The monovalent antivenom prepared from a certain snake venom has the strongest neutralization specificity for the snake venom used to prepare this antiserum, followed by other snake venoms belonging to the same family and genus, and worse or even no specificity for snake venoms of different families. In clinical application, the bitten snake species must be diagnosed before it can exert a significant curative effect. In order to effectively neutralize the snake venom of the same family or different family and genera with one kind of snake venom when it is not determined which kind of poisonous snake is bitten, scientists choose a variety of snake venoms to prepare antigens to immunize animals to prepare multivalent anti-venoms serum. For example, the polyvalent anti-rattlesnake ...

AI Technical Summary

Problems solved by technology

However, the serum is still limited to neutralizing snake venoms of the same family and genus, and the serum is only effective against rattlesnake bites in this area

Although a variety of monovalent antivenoms have been prepared in my country, there is no multivalent serum for snakebites caused by various venomous snakes in regional areas with great harm.

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