70 results about "Cellular distribution" patented technology

The present invention provides a method and / or means for collecting and analyzing an individual cell in a tissue, and at the same time, quantitatively monitoring the expression levels of various genes while keeping two-dimensional information in the tissue. Specifically, the present invention provides a method comprising preparing a cDNA library from mRNA while keeping two-dimensional cellular distribution information and obtaining the gene expression levels at any site or all sites at a level of single cell. More specifically, the present invention provides a method comprising preparing a cDNA library in a sheet-form from mRNA while keeping two-dimensional cellular distribution information and repeatedly using the cDNA library in the detection of the gene expression, thereby allowing measurement of the expression distribution for a number of genes at a high accuracy.

Methods for differentially identifying cells in an instrument employ compositions containing a combination of selected antibodies and fluorescent dyes having different cellular distribution patterns and specificities, as well as antibodies and fluorescent dyes characterized by overlapping emission spectra which form non-compensatable spectral patterns. When utilizing the compositions described herein consisting of fluorescent dyes and fluorochrome labeled antibodies with overlapping spectra that cannot be separated or distinguished based upon optical or electronic compensation means, a new fluorescent footprint is established. This new fluorescent footprint is a result of the overlapping spectra and the combined cellular staining patterns of the dyes and fluorochrome labeled antibodies chosen for the composition. The new fluorescent footprint results in histogram patterns that are useful for the identification of additional cell populations or subtypes in hematological disease.

The invention discloses an exfoliative cells preserving fluid which is prepared from a pH buffering agent, an osmotic pressure maintenance agent, preservatives, a fixing agent for maintaining cellular morphology, an anticoagulant, a mucus softener, an antimicrobial reagent, a cleaning agent, a humectant and red blood cell destroying components. The components in the preserving fluid are reasonable in proportioning, and the exfoliative cells can be preserved at a long time under normal temperature, wherein the longest time can reach 2 years; mucus can be sufficiently dissolved and the red cells can be partially destroyed; a film production effect is good, cellular distribution is very even, cellular morphology is perfect, cytoplasm and cell nucleus demarcation is distinct, gradation is clear, and cytoplasm and cell nucleus transparency is very good, and the exfoliative cells preserving fluid can be simultaneously used for the HPV-DNA (human papillomavirus-deoxyribonucleic acid), Chlamydia and immunohistochemical test; the special liquid base preserving fluid used for the cytologic examination of other parts can be provided, an imported product can be completely replaced, and the cellular constituent with diagnostic significance can be sufficiently preserved; and the exfoliative cells preserving fluid is low in configuration cost and easy to popularize.

Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device.

Methods for differentially identifying cells in an instrument employ compositions containing a combination of selected antibodies and fluorescent dyes having different cellular distribution patterns and specificities, as well as antibodies and fluorescent dyes characterized by overlapping emission spectra which form non-compensatable spectral patterns. When utilizing the compositions described herein consisting of fluorescent dyes and fluorochrome labeled antibodies with overlapping spectra that cannot be separated or distinguished based upon optical or electronic compensation means, a new fluorescent footprint is established. This new fluorescent footprint is a result of the overlapping spectra and the combined cellular staining patterns of the dyes and fluorochrome labeled antibodies chosen for the composition. The new fluorescent footprint results in histogram patterns that are useful for the identification of additional cell populations or subtypes in hematological disease.

The invention provides a description of a method and a device suitable for producing a cell suspension spray with living cells, and the produced cell preparation, suitable for grafting to a patient. In contrast to other methods, the spraying is performed through a disposable needle which is inserted into a disposable air tube; which provides a cell distribution avoiding spray nozzles. Small suspension droplets are provided instead of cell nebulization. By using medical grade sterile Luer-lock disposables from medical routine praxis, biocompatibility and easy application is addressed. In applying the method and / or in using the device, cells suitable for grafting to a patient are dispersed in a solution and sprayed with the device for distribution over the recipient graft site.

A method of detecting Alzheimer's disease includes detecting a disruption or alteration in normal sub-cellular distribution of G-protein receptor kinases (GRKs), particularly GRK2 and GRK5. The disruption is caused by abnormal accumulation of soluble β-amyloid. The prevention or suppression of the disease progression at prodromal or early stages includes correction of GRK dysfunction.

A method is described to assay for protein interactions in living cells, e.g. by the introduction of two heterologous conjugates into the cell. The method uses the measurement of cellular distribution of a detectable component (e.g. a GFP-labeled˜fluorescent probe) to indicate the presence or absence of an interaction between that component and a second component of interest. The method uses the knowledge that certain components can be stimulated to redistribute within the cell to defined locations. Inducible redistribution systems make it possible to determine if specific interactions occur between components. Inducible systems are described where it is demonstrated that the redistribution stimuli are essentially “null”, in that they affect no other system in the cell during the assay period, other than the component whose redistribution can be induced. Also described is an extraction buffer which is useful in high throughput screening for drugs which affect the intracellular distribution of intracellular components. The extraction buffer comprises a cellular fixation agent and cellular permeabilisation agent. Optimizing the composition of the extraction buffer and its application to various cell types is described.

The present invention provides a method for quantifying the amount of nucleic acid existing in a specimen which comprises steps of: (1) quantitatively measuring the amount of nucleic acid in the specimen; (2) measuring the distribution of cell types existing in the specimen; and (3) correcting the measured value of the amount of nucleic acid based on the measured value of cell distribution. According to the present invention, the expression level of genes in the target cell can be measured without isolating target cells from control cells in the sample comprising one or more types of control cells and one type of target cell.

The present invention relates to the use of and method for using MnO nanoparticles as MRI T1 contrasting agents which reduces T1 of tissue. More specifically, the present invention is directed to MRI T1 contrasting agent comprising MnO nanoparticle coated with a biocompatible material bound to a biologically active material such as a targeting agent, for example tumor marker etc., and methods for diagnosis and treatment of tumor etc. using said MRI T1 contrasting agent, thereby obtaining more detailed images than the conventional MRI T1-weighted images. The MRI T1 contrasting agent of the present invention allows a high resolution anatomic imaging by emphasizing T1 contrast images between tissues based on the difference of accumulation of the contrasting agent in tissues. Also, the MRI T1 contrasting agent of the present invention enables to visualize cellular distribution due to its high intracellular uptake. The MRI T1 contrasting agent of the present invention can be used for target-specific diagnosis and treatment of various diseases such as tumor etc. when targeting agents binding to disease-specific biomarkers are conjugated to the surface of nanoparticles.

The present invention relates to an apparatus and a method for fabricating a microcapsule, more particularly to an apparatus and a method for fabricating a microcapsule, which enable to encapsulate uniform cell number in a microcapsule through cell distribution, improve cell viability in the microcapsule through fluid exchange, and ensure uniform microcapsule size.The apparatus for fabricating a microcapsule according to the present invention uses a plurality of microchannels which are spatially connected with one another and are designed such that fluid flows through them in a particular direction, and comprises a cell supply unit which supplies a fluid mixture of cells and a cell dropletizing material; and a droplet forming unit in which a dropletization inducing fluid supplied from one of the plurality of microchannels joins with the fluid mixture of the cells and the cell dropletizing material to form a droplet.

The invention relates to a tissue engineering vessel and a preparation method and an application thereof. The tissue engineering vessel uses a tubular gelatin / polycaprolactone electricity texture membrane as a support, and smooth muscle cells are evenly distributed in the support. The preparation method includes: a) spreading disinfected gelatin / polycaprolactone materials in a culture dish; b) collecting umbilical artery smooth muscle cells, evenly inoculating the umbilical artery smooth muscle cells on the surface of the gelatin / polycaprolactone electricity texture membrane to culture over the night, and forming a cell material composite electricity texture membrane; and c) twisting the cell material composite electricity texture membrane on a polyvinyl chloride (PVC) tube for culturing, and finally forming the tubular tissue engineering vessel. Cells of the tissue engineering vessel prepared by the method are evenly distributed, simultaneously the tissue engineering vessel has the advantages of being good in elasticity, moderate in hardness and compact in tissue structure, having smooth inner walls and abundant collagenous fibers, and being clear in self tissue boundary and the like. The characteristics of the tissue engineering vessel are close to that of living body vessels.

The invention relates to a ZnO quantum dot vector / DNA composite-containing collagen-based composite cornea substitute, and a preparation method and application thereof. The ZnO quantum dot vector / DNA composite-containing collagen-based composite cornea substitute is prepared by absorbing ZnO quantum dot vectors / DNA composites by using a collagen / MPDSAH IPN cornea substitute, wherein the weight ratio of the cornea substitute to the ZnO quantum dot vectors / DNA composites is 425:1; and the weight ratio of ZnO quantum dot vectors to DNA is 25:1. The ZnO quantum dot vector / DNA composite-containing collagen-based composite cornea substitute has high biocompatibility, can induce and promote the regeneration of a cornea and be biologically decomposed along with the regeneration of the cornea, hasmechanical properties and optical properties the same as those of a human cornea, remarkably improves the stability and mechanical strength of the cornea substitute in collagenase due to the introduction of an MPDSAH polymer network, can effectively compositely compress the DNA, successfully induce the DNA into cells, successfully express the DNA, trace the position of the DNA / vector at any time and determine the intra-cellular distribution of the DNA / vector in a transgenosis process due to ZnO quantum dots, and effectively combines gene transfection, tissue engineering and fluorescent tracing so as to achieve simple manufacturing method, and easy processing and long-term storage and transport.

The invention discloses a microscopic image splicing method. The method comprises the steps: acquiring a local microscopic image sequence; carrying out cell detection on the local microscopic image toobtain a cell distribution situation, and estimating the area of a cell nucleus; according to the cell distribution condition, utilizing a brightness-based normalization cross-correlation characteristic algorithm to obtain the maximum value overlapping region and translation transformation of the adjacent local microscopic images; calculating the overlapping rate of the cell nucleus area in the maximum overlapping region according to the area of the cell nucleus, and comparing the overlapping rate with a preset overlapping rate threshold to obtain the confidence of translation transformation;according to the confidence coefficient, obtaining a splicing sequence of the local microscopic images by searching a minimum error path; and splicing the local microscopic images one by one according to the translation transformation and the splicing sequence. According to the method provided by the invention, automatic, rapid, accurate and seamless splicing of the microscopic images is realized, and seamless and accurate microscopic splicing can also be realized under the conditions of noise, blurring and extremely small overlapping area of the adjacent microscopic images.

A method of detecting parasite infection, particularly malaria, includes mixing a blood sample with a lytic reagent system to lyse red blood cells, and to form a sample mixture; performing a differential analysis of white blood cells of the sample mixture on a blood analyzer, and obtaining a cell volume distribution of a white blood cell subpopulation from a cell volume measurement used in the differential analysis; obtaining a cell volume parameter from the cell volume distribution of the white blood cell subpopulation; evaluating the cell volume parameter against a predetermined criterion, and reporting an indication of the parasite infection if the cell volume parameter meets the predetermined criterion. The method also uses a discriminant obtained from cell volume parameters of two different white blood cell subpopulations against a predetermined criterion. The method further use a cell distribution parameter of a cell distribution obtained using a RF impedance measurement.

The invention discloses a dedicated centrifugal tube and method for manufacturing cell blocks. The dedicated centrifugal tube is composed of a cylindrical centrifugal pipe with two opened ends and two centrifugal tube covers. The surfaces of the pipe walls of two ends of the centrifugal tube are provided with external threads, the centrifugal tube covers are covers with a flat bottom, the bottoms of the covers are provided with a sealing ring (cushion), the internal walls are provided with internal threads, and the internal threads and the external threads can be engaged together to form a sealed cylindrical space. The cell block is produced by the following steps: heating a centrifugal tube containing matrix to liquidize the matrix, then adding cell (cluster) suspension on the upper surface of the matrix layer, turning the centrifugal tube upside down to mix the matrix and the cell suspension, then carrying out centrifugation, (or directly carrying out centrifugation), so as to enrich the cells to a plane on the bottom layer of the matrix through the matrix (cushion); after the matrix is cured, removing the centrifugal tube cover on the two ends of the centrifugal tube, pushing out the matrix containing a cell layer by a piston or pressurized air from the centrifugal tube, and cutting the matrix to manufacture cell blocks. The provided method can conveniently and efficiently make trace cell samples into a complete and regular cell block with a large cell distribution area and high cell density.

The invention discloses a sectional non-linear enhancement method for a urinary sediment image. The method comprises the following steps of: a first step: calculating section points by using a genetic algorithm according to distribution ranges of cells in the image and background in a gray space, dividing the whole image into a background section, a target section and a transition section according to the section points, calling a gray-level zone of distributed background as the background section, counting the gray-level zone of each distributed cell, ensuring that the gray-level zones in which the backgrounds and the cells are distributed have overlapped and crossed areas to find out the most proper thresholds to divide the two zones, and selecting a gray-level zone with less than 100 pixels as the transitional section; and a second step: performing different gray-level transformation method on the background section, the target section and the transition section. The method has the beneficial effects that the enhancement method is high in processing speed and stable and reliable; and the noise of the background is well inhibited, and the whole image is obvious in vision effect.

Previously we have identified a conserved domain (SAP, for SAF-A / B, Acinus, and PIAS) in the foot-and-mouth disease virus (FMDV) leader (L) protein coding region that is required for proper sub-cellular localization and function. Mutation of isoleucine 55 and leucine 58 to alanine (I55A, L58A) within the SAP domain resulted in a viable virus that displayed a mild attenuated phenotype in cell culture, along with altered sub-cellular distribution of L and failure to induce degradation of the transcription factor nuclear factor kappa-B. Here we report that inoculation of swine and cattle with this mutant virus results in the absence of clinical disease, the induction of a significant FMDV-specific neutralizing antibody response, and protection against subsequent homologous virus challenge. Remarkably, swine vaccinated with SAP mutant virus are protected against wild type virus challenge as early as two days post-vaccination suggesting that a strong innate as well as adaptive immunity is elicited. This variant could serve as the basis for construction of a live-attenuated FMD vaccine candidate.

Provided are systems for analyzing cellular distribution in an engineered tissue sample, which optionally is a bioprinted organ or tissue sample. In some embodiments, the systems include an ultrasound imaging system and a processing unit configured with software that permits analysis of images acquired from the engineered tissue sample in order to output desired characteristics thereof. In some embodiments, the systems also include a bioreactor for engineering a tissue sample and a pump configured to regulate flow of fluids and reagents into and out of the bioreactor, wherein at least one surface of the bioreactor includes a window that is acoustically transparent to ultrasound waves. Also provided are systems for analyzing cell distribution in an engineered tissue sample and methods for analyzing distribution of cells in an engineered tissue sample present within a bioreactor.

The invention discloses a bioreactor cell culture quality evaluation method, and relates to the technical field of bioreactors. The bioreactor cell culture quality evaluation method comprises image enhancement, image segmentation, smoothing processing, connected region extraction, standard cell area judgment and cell distribution statistics, and completes automatic evaluation of the cell culture quality through statistics of the number of cells. According to the bioreactor cell culture quality evaluation method, the growth state of the cultured cells can be automatically evaluated, and a reliable guarantee means is provided for promoting the application of a large-scale cell culture technology in a cell factory.

An electroporation cuvette is constructed with electroporation electrodes arranged in non-parallel relation to form a gap whose width varies with the location within the cuvette, plus a pair of positioning electrodes that are arranged to cause electrophoretic migration of biological cells within the cuvette according to cell size. Once the cells, suspended in a solution of the impregnant, are distributed in the cuvette by the positioning electrodes, electric field pulses are generated by the non-parallel electroporation electrodes. Because of their distribution in the cuvette, the various cells will experience voltage differentials across their widths that approach uniformity regardless of cell diameter, since the larger cells will be positioned at locations where the gap between the electrodes is greater and the smaller cells at locations where the gap is relatively small while the voltage drop across the entire gap is uniform along the length of the cell. The voltage differential across the width of the cell is thus roughly paired with the cell diameter, and this reduces the disparity in voltage differential that cells of different sizes would otherwise experience with parallel electrodes.

A characteristic data pre-processing system includes a data acquisition device that collects characteristic data including first cell distribution data defined according to first default read levels, and second cell distribution data defined according to second default read levels, a data pre-processing apparatus that merges the first cell distribution data and the second cell distribution data according crop ranges to generate training data, wherein the crop ranges are defined according to the first default levels and the second default levels, and a database that stores the training data communicated from the data pre-processing apparatus.

Embodiments of the invention relate to devices and methods for evaluating the interactions between cells and between cells and matrix materials wherein the cellular distribution patterns formed as a result of such interactions are indicators of the invasive potential(s) of the cells. Furthermore, such devices and methods can provide indications of the preferred sites of metastasis of invasive cells; the efficacy of an anti-cancer drug applied to such cells; and the potential for agents to promote or enhance tumor growth or metastasis.

The invention discloses a 5G tracking area abnormity detection method and system based on a cellular distribution identification technology, and the method comprises the steps: carrying out the TAC relation analysis of a main cell and a peripheral adjacent cell according to the TAC map distribution rule of base stations and cells with the distance between the base stations and four quadrants of the center coordinate axis of the base stations as entry points; establishing two TAC base station models, and accurately identifying cells with abnormal TAC according to different scenes; performing modeling based on the TAC map distribution rule of the base stations and the cells, and the cells with abnormal TAC can be accurately identified according to the actual map distribution of the base stations. According to the method, the cell with the abnormal TAC is rapidly identified, and operation and maintenance personnel are helped to timely troubleshoot mobile network risks and hidden dangers.

The embodiment of the invention discloses a method for establishing a standardized tumor animal model and a culture device. The method comprises the following steps that a 3D cell ring culture mold ispretreated, and the pretreated 3D cell ring culture mold is placed into a cell culture dish; the 3D cell ring culture mold is obliquely rotaed in the space of the cell culture dish according to different sequences, and 3D tumor cell rings with uniformly distributed tumor cells are cultured on the 3D cell ring culture mold; and the 3D tumor cell ring is cultured in vitro until the 3D tumor cell ring is formed, the 3D tumor cell ring is transplanted into the animal tissue for secondary culture, and the tumor cells of the 3D tumor cell ring are enabled to bidirectionally grow at the modeling position of the animal tissue until the tumor grows and is formed. According to the scheme, cell loss is little, the tumor formation rate is high, tumor formation is uniform and stable, the number of thecells required by modeling and the modeling cost are greatly reduced, and the method for establishing the standardized tumor animal model is efficient, simple, reliable, high in operability, economical and practical.

The invention relates to the technical field of medical auxiliary diagnosis, and discloses an artificial intelligence blood smear cell labeling system and method. The artificial intelligence blood smear cell labeling system comprises an observation unit, a labeling unit, an analysis and classification unit and a central control unit; wherein the observation unit comprises a microscope, a CCD camera and a microscopic control module; the labeling unit comprises a display module and a labeling operation module, and the labeling operation module comprises a labeling line adding module and a standard line deleting module; and the analysis and classification unit comprises a cell distribution statistical template, a cell quantity statistical module, a purple cell statistical module, an orange cell statistical module, a huge cell statistical module, a cell cluster analysis module, a benign cell statistical module and an abnormal cell statistical module. According to the method, the multiple of the microscope can be automatically adjusted, the clearest image in the same visual field image is found around the set multiple and cells in the image are marked, wherein the benign cells enter a benign disease classification system, and abnormal cells enter a pathology classification system.

The present invention is directed to transduced T cells expressing at least 100,000 molecules of human somatostatin receptor 2 (SSTR2), which improves PET / CT imaging sensitivity. The present invention is also directed to transduced T cells expressing SSTR2 and chimeric antigen receptor (CAR). In one embodiment, the CAR is specific to human ICAM-1 and the CAR comprises a binding domain that is scFv of anti-human ICAM-1, or an I domain of the αL subunit of human lymphocyte function-associated antigen-1. In another embodiment, the CAR is specific to human CD19, and the CAR comprises a binding domain that is scFv of anti-human CD19. The present invention is further directed to using the above transduced T cells for monitoring T cell distribution in a patient by PET / CT imaging and / or treating cancer.

The invention discloses a cell classification result correction method based on multi-peak fitting analysis, and the method comprises the following steps: classifying various cells by using an automatic classification algorithm according to sample cell characteristics collected by a cell analyzer; according to an automatic classification result, extracting a feature histogram of overlapped cell classification, and extracting a noise signal obtained by Gaussian filtering elimination sampling with a step length of 3 or 5 in the process; estimating Gaussian distribution parameters according to the cell distribution, and respectively executing coarse Gaussian distribution fitting on each wave crest; on the basis of the rough fitting result, executing overlapped Gaussian distribution fitting, and obtaining the distribution parameters of cell peaks; selecting an overlapping region of each cell peak, calculating the area ratio of the overlapping region, completing cell content correction, and improving the classification accuracy.