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Bioreactor cell culture quality evaluation method

A bioreactor and cell culture technology, applied in the field of bioreactor cell culture quality evaluation, can solve the problems of objective accuracy, no objective evaluation method, and inability to guarantee evaluation results, etc., and achieve the effect of reliable guarantee means

Pending Publication Date: 2020-03-24
CHANGCHUN UNIV OF SCI & TECH
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Problems solved by technology

However, the current evaluation methods still mainly rely on experience to judge the state of cell culture. There is no objective evaluation method, and the objective and accurate evaluation results cannot be guaranteed.

Method used

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  • Bioreactor cell culture quality evaluation method
  • Bioreactor cell culture quality evaluation method
  • Bioreactor cell culture quality evaluation method

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Embodiment Construction

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0024] The invention provides a method for evaluating the quality of cell culture in a bioreactor. The main flow of the method is as follows: firstly, it is necessary to use image enhancement technology to highlight the useful information in the image, weaken or even eliminate useless information, and then use image segmentation technology to separate the cells in the image. Separate from the background or other cells, and then through image smoothing, retain t...

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Abstract

The invention discloses a bioreactor cell culture quality evaluation method, and relates to the technical field of bioreactors. The bioreactor cell culture quality evaluation method comprises image enhancement, image segmentation, smoothing processing, connected region extraction, standard cell area judgment and cell distribution statistics, and completes automatic evaluation of the cell culture quality through statistics of the number of cells. According to the bioreactor cell culture quality evaluation method, the growth state of the cultured cells can be automatically evaluated, and a reliable guarantee means is provided for promoting the application of a large-scale cell culture technology in a cell factory.

Description

technical field [0001] The invention relates to the technical field of bioreactors, in particular to a method for evaluating the quality of bioreactor cell culture. Background technique [0002] A bioreactor is a device that achieves in vitro culture by simulating the in vivo growth environment of enzymes or organisms (such as cells, microorganisms, etc.) through biological reactions or self-metabolism. Bioreactors play an important role in vaccine production, monoclonal antibody preparation, pharmaceutical production, tumor prevention and treatment, wine making, biological fermentation, and degradation of organic pollutants. [0003] In the process of using bioreactors for cell culture, it is necessary to evaluate the quality of cell culture. However, the current evaluation methods still mainly rely on experience to judge the state of cell culture, there is no objective evaluation method, and the objective and accurate evaluation results cannot be guaranteed. Contents of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/00G06T7/11G06T7/13G06T7/136G06T7/187G06T5/00G06K9/00G06K9/62
CPCG06T7/0012G06T7/11G06T7/13G06T7/136G06T7/187G06T2207/10024G06T2207/10056G06T2207/30004G06T2207/30242G06V20/69G06F18/23G06F18/24G06T5/70
Inventor 宫平郭红壮马辰昊葛辉琼张宁阚宝慧朱海焕吴昊李旭吉翔宇谭国桢郭旭
Owner CHANGCHUN UNIV OF SCI & TECH
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