The invention discloses a breeding method for transgenic pigs expressing sIFITM3 (swine
interferon induced transmembrane 3) genes. The breeding method comprises the steps as follows: firstly, the construction of swine
fibroblast cell lines stably expressing the sIFITM3 genes comprises the following steps: the step a refers to the construction and the
linearization of pcDNACAsIITM3 vectors, wherein, primers are designed,
lymph node tissue
RNA (
Ribose Nucleic Acid) of pigs is extracted, the sIFITM3 genes are obtained through RT-PCR (Reverse Transcription-
Polymerase Chain Reaction) amplification,
ethanol precipitation is carried out, and
sterile water is used for dissolving; the step b refers to
liposome transfection, wherein, swine fibroblasts expressing sIFITM3 are constructed; the step c refers to screening and proliferation of nuclear donor cells transfected with the sIFITM3 genes; secondly, recombination
blastula acquisition based on a manual
nucleus transplantation method comprises the following steps: the step a refers to the preparation of recipient cells; the step b refers to enucleation and injection of the nuclear donor cells; and thirdly, the
embryo transplantation and the acquisition of the transgenic pigs comprise the following steps: the step a refers to
embryo transplantation, wherein, bred blastulas are transferred to a
fallopian tube of a surrogate sow, and then detection is carried out; and the step b refers to transgenic individual identification. The breeding method is feasible, and is simple and convenient to operate; in addition, the pigs are the transgenic pigs expressing sIFITM3, and have the potential capability to
resist animal virus such as foot-and-mouth
disease virus,
Japanese encephalitis virus, swine influenza
virus and the like.