The invention discloses a triple artificial miRNA carrier in restraining of VEGFRs and a construction method and application thereof. According to the triple artificial miRNA carrier in restraining of VEGFRs, artificial miRNAs are constructed in a pcDNATM6.2-GW / miR carrier and targeted to three target genes, namely VEGFR1, VEGFR2 and VEGFR3, the three artificial miRNAs are connected in series to be connected to a skeleton carrier of psliencer4.1 to form the triple amiRNA carrier, and the triple amiRNA carrier exerts the effect of silencing the three genes, namely VEGFR1, VEGFR2 and VEGFR3 at the same time. The triple amiRNA reduces proliferation of pancreatic cancer cells, accelerates apoptosis, and reduces the capacity of cell migration and invasion; in a nude mouse pancreatic cancer transplanting model, the triple amiRNA-VEGFRs obviously reduces the growth speed of a tumor, achieves a synergistic effect with chemotherapy drugs, and is related to restraining of epithelium-mesenchyme conversion; the pancreas shape and the insulin and blood glucose level of peripheral blood are not influenced. In this way, the triple artificial miRNA in restraining of VEGFRs can be well applied to preparation of medicine for treating a pancreatic cancer and other malignant tumors with high expression VEGFRs.