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Application of tiRNA as drug target in metastasis treatment of colorectal cancer

A colorectal cancer and therapeutic target technology, applied in the field of biomedicine, can solve problems such as unclear mechanism of action and clinical significance

Inactive Publication Date: 2019-07-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the mechanism and clinical significance of tsRNA, especially tiRNA, in colorectal cancer are still unclear and need to be clarified

Method used

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  • Application of tiRNA as drug target in metastasis treatment of colorectal cancer
  • Application of tiRNA as drug target in metastasis treatment of colorectal cancer
  • Application of tiRNA as drug target in metastasis treatment of colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] Example 1: Expression of tiRNA in cancer tissues of patients with colorectal cancer

[0114] 1. Analysis of differential expression of tiRNA in paracancerous tissue and cancer tissue of colorectal cancer patients

Embodiment approach

[0115] Implementation method: The small non-coding RNA high-throughput sequencing method was used to detect the expression of tiRNAs in the paracancerous tissues and cancer tissues of 3 patients with colorectal cancer, and then the differentially expressed tiRNAs were analyzed by bioinformatics.

[0116] Result analysis: if figure 1It was shown that, compared with paracancerous tissues, 5'-tiRNAs significantly highly expressed in colorectal cancer tissues included 5'-tiRNA-Val-AAC, 5'-tiRNA-Ala-AGC, 5'-tiRNA-Pro-AGG , 5'-tiRNA-Val-CAC, 5'-tiRNA-Leu-CAG, 5'-tiRNA-Glu-CTC and 5'-tiRNA-Cys-GCA; significantly lower expressed 5'-tiRNAs include 5'- tiRNA-Gly-TCC, 5'-tiRNA-His-GTG, 5'-tiRNA-SeC-TCA and 5'-tiRNA-Tyr-GTA; significantly high expressed 3'-tiRNA including 3'-tiRNA-Ala- TGC, 3'-tiRNA-Ala-CGC, 3'-tiRNA-Gly-CCC, 3'-tiRNA-Val-TAC, 3'-tiRNA-Ala-AGC, and 3'-tiRNA-Met-CAT; low significance The expressed 3'-tiRNAs included 3'-tiRNA-Thr-TGT, 3'-tiRNA-Trp-CCA, 3'-tiRNA-Arg-ACG an...

Embodiment 2

[0121] Example 2: tiRNA can promote the metastasis and invasion ability of colorectal cancer cells

[0122] 1. Screening of colorectal cancer cell lines with high and low migration and invasion ability

[0123] Implementation method: Based on the Transwell migration and invasion assay, the colorectal cancer cells (HCT116) in the upper and lower layers of the Transwell chamber were isolated and cultured, and the isolation and culture method was based on the existing report MiR-218 inhibits invasion and metastasis of gastric cancer by targeting the Robo1receptor (PLoS Genet 2010; 6: e1000879); after 3 rounds of screening (the screening condition is whether the cells can migrate to the lower layer of the Transwell chamber), the cells that meet the conditions of migrating to the lower layer of the Transwell chamber and staying in the upper layer of the Transwell chamber are selected to screen and establish two different migration and invasion abilities. The sub-cell lines were nam...

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Abstract

The invention belongs to the field of biomedicine, relates to a novel target for colorectal cancer metastasis treatment, in particular to application of tiRNA as the target in the metastasis treatmentof the colorectal cancer, and also relates to the tiRNA and an antagonist siRNA and the application using the the tiRNA and the antagonist siRNA to prepare medicines for targeted treatment of colorectal cancer metastasis. The invention discloses the use of the tiRNA as the treatment target for the colorectal cancer metastasis or as a medicament for regulating and treating the colorectal cancer metastasis. The antagonist of 5'-tiRNA has a great clinical value in the preparation of pharmaceutical compositions for treating the colorectal cancer, and provides novel drugs and methods for effectivetreatment of the colorectal cancer.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a new target for the treatment of colorectal cancer metastasis, specifically, it relates to using tiRNA as a target for the treatment of colorectal cancer metastasis, and also relates to tiRNA and its antagonist siRNA, including using them to prepare drugs as Application of targeted therapy for colorectal cancer metastasis. Background technique [0002] Malignant tumor is a disease that seriously endangers human life and health. In my country, about 3.07 million people suffer from cancer every year, and about 2.2 million people die from cancer every year. According to the 2012 World Cancer Report of the World Health Organization, there were 3.07 million newly diagnosed cancer cases in China, accounting for 21.8% of the global total; and 2.2 million annual deaths, accounting for 26.9% of the global annual cancer deaths. What's more serious is that these data are increasing year by year a...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K31/713A61P35/04C12Q1/6886
CPCA61K31/713A61K45/00A61P35/04C12Q1/6886C12Q2600/106C12Q2600/178
Inventor 盛静浩许正平李斯琪
Owner ZHEJIANG UNIV
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