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Preparation method of placenta-source decidua parietalis mesenchymal stem cells by separation and refrigeration

A technology of mesenchymal stem cells and parietal decidua, applied in the field of cell biology, can solve the problems that bone marrow-derived MSCs are difficult to achieve, and achieve the effects of ensuring cell uniformity and effectiveness, improving cell culture quality, and good growth

Inactive Publication Date: 2016-01-13
SHANGHAI BIOZONE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, DMSC can be cryopreserved like UCMSC and can be used to establish a cell bank, while bone marrow-derived MSC is difficult to achieve

Method used

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  • Preparation method of placenta-source decidua parietalis mesenchymal stem cells by separation and refrigeration
  • Preparation method of placenta-source decidua parietalis mesenchymal stem cells by separation and refrigeration
  • Preparation method of placenta-source decidua parietalis mesenchymal stem cells by separation and refrigeration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] This embodiment provides a preparation method for separating and freezing placenta-derived decidual mesenchymal stem cells, which specifically includes the following steps:

[0049] Step 1. Placenta screening and preservation: screening and collection of placenta No. 1 of healthy full-term maternal volunteer baby boys, placing it in a placenta preservation box at 4°C, and separating and processing within 24 hours; the screening test items include carrying Viruses, genetic family history, etc.; such viruses as hepatitis B, hepatitis C, AIDS, syphilis, etc.;

[0050] At the same time, a tube of maternal blood was collected for re-examination. The re-examination items were viral immunological detection and genetic detection of hepatitis B, hepatitis C, AIDS, and syphilis.

[0051] Step 2, placenta separation: transfer the placenta to a petri dish, wash with normal saline; peel off the decidua wall; tear off the

[0052] The parietal decidua was placed in a centrifuge tube...

Embodiment 2

[0061] This embodiment provides a preparation method for separating and freezing placenta-derived decidual mesenchymal stem cells, which specifically includes the following steps:

[0062] Step 1. Placenta screening and storage: screening and collection of placenta No. 2 of healthy full-term maternal volunteer baby boys, placing it in a placenta storage box at 4°C, and separating and processing within 24 hours; the screening test items include carrying Viruses, genetic family history, etc.; such viruses as hepatitis B, hepatitis C, AIDS, syphilis, etc.;

[0063] At the same time, a tube of maternal blood was collected for re-examination. The re-examination items were viral immunological detection and genetic detection of hepatitis B, hepatitis C, AIDS, and syphilis.

[0064] Step 2. Placenta separation: transfer the placenta to a petri dish, wash with normal saline; peel off the decidua; place the torn off decidua in a centrifuge tube and cut it to 1-4mm 3 Small piece;

[00...

Embodiment 3

[0073] This embodiment provides a preparation method for separating and freezing placenta-derived decidual mesenchymal stem cells, which specifically includes the following steps:

[0074] Step 1. Placenta screening and storage: screening and collection of placenta No. 3 of healthy full-term maternal volunteer baby boys, placing it in a placenta storage box at 4°C, and separating and processing within 24 hours; the screening test items include carrying Viruses, genetic family history, etc.; such viruses as hepatitis B, hepatitis C, AIDS, syphilis, etc.;

[0075] At the same time, a tube of maternal blood was collected for re-examination. The re-examination items were viral immunological detection and genetic detection of hepatitis B, hepatitis C, AIDS, and syphilis.

[0076] Step 2. Placenta separation: transfer the placenta to a petri dish, wash with normal saline; peel off the decidua; place the torn off decidua in a centrifuge tube and cut it to 1-4mm 3 Small piece;

[0077...

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Abstract

The invention provides a preparation method of placenta-source decidua parietalis mesenchymal stem cells by separation and refrigeration. According to the preparation method, puerpera is screened before placenta acquisition, and placenta from healthy mature puerperas volunteers who give birth to baby boys is finally selected as the acquisition target; separation and amplification are carried out for large-scale culture until the third generation, and refrigeration and preservation are carried out; and an intermediate product obtained from the separation process and refrigeration process undergoes a series of quality detections such as maternal detection, and cells are resurrected and washed for later use after refrigeration. There is no obvious reduction of motility rate, and biological functions of cells are complete. The placenta-source decidua parietalis mesenchymal stem cells have a simple component, and the whole process from raw material acquisition to cell preparation is monitored clinically, unitedly and systematically so as to realize a clinic standard product of mesenchymal stem cells. Besides, decidua parietalis comes from mother and is more suitable to be used by maternal body.

Description

technical field [0001] The invention belongs to the field of cell biology, and in particular relates to a preparation method for separating and freezing placenta-derived decidua mesenchymal stem cells. Background technique [0002] Stem cells (StemCells, SC) are a kind of pluripotent cells with self-renewing ability (self-renewing), which are primitive and unspecialized cells. Under certain conditions, it can differentiate into a variety of functional cells, and has the potential to regenerate various tissues and organs and the human body. Stem cells exist in all multicellular tissues, can divide into a variety of specialized cells through mitosis and differentiation, and can use self-renewal to provide more stem cells. There are many sources of stem cells, including placenta, umbilical cord, umbilical cord blood and bone marrow. [0003] Decidual mesenchymal stem cells (Decidua-derived mesenchymal stem cells, DMSCs) not only have the general phenotype and differentiation ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A01N1/02
Inventor 苏量
Owner SHANGHAI BIOZONE BIOTECH CO LTD
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