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64 results about "Glucoraphanin" patented technology

Glucoraphanin is a glucosinolate found in broccoli, cauliflower, and mustard. Glucoraphanin is converted to sulforaphane by the enzyme myrosinase. In plants, sulforaphane deters insect predators and acts as a selective antibiotic. In humans, sulforaphane has been studied for its potential affects in neurodegenerative and cardiovascular diseases.

Method for preparing glucoraphanin from broccoli

The invention discloses a method for preparing glucoraphanin from broccoli, which comprises the following steps: cleaning the raw material, chopping, adding into an extraction tank set host, and sufficiently contacting solid-phase and liquid-phase substances in the extraction tank set in a reverse motion process, thereby extracting effective components from the medicinal material. The medicine slag are forcedly pushed to a slag outlet to be discharged by a discharge conveyer, and a special squeezing machine extrudes the medicine slag to obtain medicinal material tissues from the residual liquor in the medicine slag, thereby reducing the residual liquor content in the medicine slag. The method has the advantages of higher extraction efficiency and more complete extraction. By adopting the macroporous adsorbent resin combined with silica gel column chromatography for purification, the separating effect is better. The method can be used for preparing the glucoraphanin finished product of which the maximum yield is 1% and the content is higher than 95%.
Owner:HEILONGJIANG DINGHENGSHENG PHARM CO LTD

Method for preparing radish red pigment homopolymer and radish proanthocyanidin from red-core radishes

The invention relates to a method for preparing radish red pigment homopolymer and radish proanthocyanidin from red-core radishes, belonging to the technical field of preparation of natural red pigments and proanthocyanidin. The method comprises the following steps: by using red-core radishes as the raw material, extracting, removing proteins, removing glucoraphanin, purifying by ultrafiltration,concentrating, and carrying out spray drying to obtain the radish red pigment homopolymer and radish proanthocyanidin products. The invention has the advantages of great convenience, simple technique, wide raw material sources, and cheap and accessible raw materials; all the materials in the production process can be recycled, so that the material resources are fully utilized and no waste is discharged, and thus, the invention also has the advantages of energy saving and low production cost; and the products have the advantages of high purity, no peculiar smell of radishes, favorable solubility and the like. The invention can be widely used for preparing radish red pigment homopolymer and radish proanthocyanidin from red-core radishes. The products prepared by the method provided by the invention can be widely used in the industries of food, medicines, health products, cosmetics, dyes and the like.
Owner:重庆海巨农业发展有限公司 +1

Glucoraphanin-containing composition and use thereof

The invention relates to a glucoraphanin-containing composition which contains a component 1 for providing glucoraphanin, a component 2 for providing myrosinase and a basic salt compound. The composition disclosed by the invention has good stability and overcomes the problems that the content of the glucoraphanin is lowered, a product is discolored and tastes bitter and the like in the prior art.The invention further relates to a pharmaceutical use of the composition.
Owner:SHENZHEN F&S BIO TECH

Method for extracting and separating glucoraphanin from broccoli seeds

The invention relates to a method for extracting and separating glucoraphanin from broccoli seeds. The method comprises the following steps that 1, a glucoraphanin solution is extracted from the broccoli seeds and concentrated to obtain a concentrated glucoraphanin solution; 2 the concentrated glucoraphanin solution is adsorbed through macroporous adsorption resin; 3, the macroporous adsorption resin is eluted with an ethanol solution to obtain resin eluate; 4, after the resin eluate is concentrated, silica gel is added, mixing is performed, ethanol is volatilized, and silica gel adsorbing glucoraphanin is obtained and added to the upper layer of a silica gel column obtained through silica gel column filling; 5, the silica gel column is eluted with eluent, eluate is collected, concentrated and dried, and then the glucoraphanin product is obtained. Compared with the prior art, the method has the advantages that the technology is simple and easy to operate, industrialization is easy, the macroporous resin and the silica gel are low in cost and easy to obtain, the whole technology is low in price and little in investment, and the high-purity glucoraphanin product can be obtained.
Owner:PIONEER HERB IND CO LTD

Foliar fertilizer and method for improving contents of glucoraphanin and organic selenium in broccoli

InactiveCN108848836AIncreased glucoraphanin contentMagnesium fertilisersFertilising methodsGlucoraphaninSe element
The invention discloses a fertilizer capable of simultaneously improving contents of glucoraphanin and organic selenium in broccoli. The sulfur content in the fertilizer is 1 to 4 mM, and the seleniumcontent in the fertilizer is 50 to 150 mu M. Meanwhile, the invention further provides a method capable of simultaneously improving contents of glucoraphanin and organic selenium in broccoli. The method comprises the step of foliage spraying of the fertilizer on broccoli plants. By means of the foliar fertilizer disclosed by the invention, the content of glucoraphanin in the broccoli is effectively improved by 27.9 to 89.5%, and the content of organic selenium in the broccoli is improved by 5.1 to 16.5 times.
Owner:HUNAN AGRICULTURAL UNIV

Method for extracting sulforaphane and myrosinase magnetic microspheres

The invention discloses a method for extracting sulforaphane and myrosinase magnetic microspheres, the method comprises the following steps of:(1) heating and stir-frying broccoli seeds, and squeezingoil to remove grease to obtain an oil cake;(2) crushing the oil cake into small particles, soaking and extracting in water to obtain a sulforaphane extract;(3) adding myrosinase magnetic microspheresinto the sulforaphane extract, performing enzymolysis, and recovering the myrosinase magnetic microspheres by magnet adsorption to obtain sulforaphane extract;(4) extracting the sulforaphane by usingdichloromethane to obtain a dichloromethane extract containing the sulforaphane, and concentrating the dichloromethane extract;(5) dissolving the concentrated solution by using acetonitrile, pouringout supernatant, and concentrating to obtain concentrated solution; (6) dissolving the concentrated solution obtained in step (5) by using edible ethanol, pouring out supernatant, and concentrating toobtain the sulforaphane. According to the method, by taking the solidified myrosinase magnetic microspheres as a catalyst, the glucoraphanin is hydrolyzed into sulforaphane, and the purity of the sulforaphane is as high as 95%.
Owner:LESHAN NORMAL UNIV +1

Method for detecting oxidized form glucoraphanin and reduced form glucoraphanin from radish extract

ActiveCN106501419ARealize simultaneous quantitative detectionReduce testing costsComponent separationGlucoraphaninPeak area
The invention discloses a method for detecting oxidized form glucoraphanin and reduced form glucoraphanin from a radish extract. The method comprises the following steps: dissolving an oxidized form glucoraphanin and reduced form glucoraphanin standard product into a methanol-aqueous solution, and diluting to obtain a standard product solution with series mass concentration; dissolving the to-be-tested radish extract into a methanol-aqueous solution to obtain a radish extract sample solution; performing high performance liquid chromatography analysis on the standard product solution with series mass concentration and the radish extract sample solution separately to obtain each chromatographic peak area; establishing standard curve equations of the oxidized form glucoraphanin and the reduced form glucoraphanin separately; and substituting the chromatographic peak area of the oxidized form glucoraphanin and the chromatographic peak area of the reduced form glucoraphanin in the sample solution into the corresponding curve equations to obtain the mass concentration of the two types of glucoraphanin in the sample solution, and calculating to obtain the oxidized form glucoraphanin content and the reduced form glucoraphanin content of the radish extract. The method realizes synchronous and quantitative detection of the oxidized form glucoraphanin and the reduced form glucoraphanin, and has the advantages of high speed, high efficiency and high accuracy.
Owner:LINYI UNIVERSITY

Method for producing high-purity sulforaphene by using glucoraphanin

InactiveCN109593798AAchieve in situ separationReduce inhibitionFermentationDialysis membranesGlucoraphanin
The invention belongs to the technical field of sulforaphene production, and particularly relates to a method for producing high-purity sulforaphene by using glucoraphanin. According to the method, the sulforaphene is prepared by taking glucoraphanin, myrosinase and a dialysis membrane tube as raw materials through the steps of glucoraphanin reaction solution preparation, reaction system preparation, dialysis reaction, reverse osmosis concentration, centrifugal extraction and the like. According to the method, the dialysis membrane tube is introduced for dialysis reaction, so that the in-situseparation of the product from the substrate and enzyme is realized, the degradation rate of the glucoraphanin is significantly improved, the complete retention of the myrosinase is realized, and thedialysis membrane tube can be repeatedly used for the production of the sulforaphene. The method has the characteristics of being simple in production process, stable, easy in organization amplification, high in product yield, low in equipment investment cost, low in production cost, high in comprehensive resource utilization rate and environmentally friendly. The sulforaphene prepared by the method has the characteristics of being high in purity, free of solvent residue and stable in performance, and can be widely used in the industries of medicine, food and daily chemical.
Owner:CHONGQING TECH & BUSINESS UNIV

Pharmaceutical composition for treating diabetes mellitus

The invention discloses a pharmaceutical composition for treating diabetes mellitus and a preparation method thereof. The pharmaceutical composition is prepared from the following medicinal raw materials in a proportion: gomisin G, mucuna birdwoodiana, herba artemisiae adamsii, glucoraphanin, swertiamarin and sarmentosin. The pharmaceutical composition can be manufactured into various preparations according to a conventional preparation process, and has a remarkable curative effect for treating diabetes mellitus.
Owner:JINAN XINGYI MEDICAL TECH

Breeding method of broccoli variety with high glucoraphanin content in broccoli florets and seeds

The invention discloses a breeding method of a broccoli variety with high glucoraphanin content in broccoli florets and seeds. According to the invention, a broccoli double haploid (DH) line obtainedby microspore culture is used as a male parent, a cytoplasmic male sterile line of a high-glucoraphanin-content broccoli variety obtained by multi-generation backcross transformation is used as a female parent, and sexual hybridization is performed to obtain the novel high-glucoraphanin-content broccoli variety. According to the high-glucoraphanin-content broccoli variety obtained through breedingin the invention, the content of glucoraphanin in the broccoli florets is 2.5 times or above the content of glucoraphanin in a common variety (a commercially-available variety with the highest glucoraphanin content), the content of glucoraphanin in the broccoli seeds is 107 micromole / g or above, and the broccoli seeds do not contain alkenylated glucosinolate components.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES

Method for preparing reduced type glucoraphanin by enteric bacterium metabolism and detection method

The invention provides a method for preparing reduced type glucoraphanin by enteric bacterium metabolism and a detection method. The method comprises the following steps: preparing an anaerobic culture solution and an intestinal bacteria solution, mixing and incubating the anaerobic culture solution and the intestinal bacteria solution to obtain an intestinal bacteria culture solution, then addinga glucoraphanin extract into the intestinal bacteria solution, thus obtaining a crude product solution containing metabolite under the action of intestinal flora and utilizing sephadex to separate and purify the metabolite to obtain the highly purified reduced type glucoraphanin. The reduced type glucoraphanin obtained by the invention has high degree of purity and a good purification effect.
Owner:SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE

A method of preparing a novel cauliflower variety rich in glucoraphanin by utilizing molecular marking

A method of preparing a novel cauliflower variety rich in glucoraphanin by utilizing molecular marking is disclosed. Through a process of crossing, selfing and back-crossing in order, based on a molecular marking technique, and through combining marker assisted selection and traditional phenotypic selection, related gene fragments in a broccoli selfing line having a high glucoraphanin content are transferred to a cauliflower selfing line having excellent comprehensive properties to obtain the novel cauliflower variety having a high glucoraphanin content. By utilizing molecular marking, single plants having high glucoraphanin contents can be selected in a seedling stage and single plants having low glucoraphanin contents are eliminated, the area of land utilized is small, the working load is low, and disadvantages such as long cycles, high working loads and susceptibility to environment influences of methods for measuring glucoraphanin contents of ball-flowers in conventional breeding methods can be overcome. Different cauliflower selfing lines can be subjected to high-glucoraphanin-content creation so that passive glucoraphanin content breeding is turned into initiative breeding and creation, thus providing rich original materials for breeding the novel cauliflower variety having the high glucoraphanin content.
Owner:SHANGHAI ACAD OF AGRI SCI +1

Fertilizer for improving broccoli yield and quality and preparation method thereof

The invention provides a fertilizer for improving broccoli yield and quality and a preparation method thereof. The preparation method comprises the following steps: firstly, mixing porous micro-powderprepared from corn straw powder with cottonseed hull powder to obtain mixed powder, spraying and granulating the mixed powder and polyglutamic acid to prepare particles, pouring the particles into anaqueous solution prepared from an inorganic fertilizer component, mixed bacterial powder and a corn-enteromorpha clathrata mixed fermentation product, and carrying out dipping treatment, and finally,performing vacuum freeze-drying to obtain the fertilizer, the fertilizer is particularly suitable for broccoli planting, the broccoli yield is effectively increased, the content of glucoraphanin, vitamin C and the like of the mature broccoli is greatly increased, and high-quality broccoli is harvested.
Owner:崔艳兰
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