Breeding method of broccoli variety with high glucoraphanin content in broccoli florets and seeds

A technology of glucoraphanin and broccoli, applied in the directions of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of goiter and limited efficacy, achieve high homozygosity, neat hybrids, and reduce seed production. cost effect

Inactive Publication Date: 2020-10-30
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a variety of glucosinolate components can be detected in broccoli germplasm, and thioalkylglucosinolates such as glucoraphanin are generally considered to be beneficial components for health, while alkenylated glucosinolate components have limited efficacy, especially Oxazolidine-2-thione, a hydrolyzate of 2-hydroxy-3-butenylglucosinolate, can cause goiter in some mammals

Method used

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  • Breeding method of broccoli variety with high glucoraphanin content in broccoli florets and seeds
  • Breeding method of broccoli variety with high glucoraphanin content in broccoli florets and seeds
  • Breeding method of broccoli variety with high glucoraphanin content in broccoli florets and seeds

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Embodiment 1

[0040] like figure 1 As shown, the present embodiment specifically provides a breeding method of a cauliflower curd and grain high glucoraphanin content variety 'Zheqing 803', comprising the following steps:

[0041] 1. Female parent selection

[0042] 1.1 After identification, broccoli inbred line 'IL609' is high glucoraphanin curd germplasm and high glucoraphanin grain material.

[0043] 1.2 The microspore culture of 'IL609' was carried out, and a total of 22 DH strains were obtained, and the high glucoraphanin material was identified and named 'DH803'.

[0044] 1.3 Using the cytoplasmic male sterile broccoli variety 'Excellent' with excellent traits as the female parent and the high glucoraphanin broccoli 'DH803' as the male parent, cross-pollinated to obtain the first generation of hybrid (F 1 ).

[0045] 1.4 Using the high glucoraphanin ‘DH803’ as the recurrent parent, carry out multi-generation backcross breeding, and select and identify excellent sterile single plant...

Embodiment 2

[0062] Embodiment 2 seed production technology

[0063] 1. Parental breeding

[0064] Seed production methods in plastic greenhouses. The male parent directly collects seeds in a closed greenhouse, and self-fertilization is strong. The female sterile line 'DH803A' and the corresponding maintainer line 'DH803' were planted at a ratio of 2:1. The bumblebee was released for pollination at the flowering stage, and the maintainer line was removed at the horn formation stage. The seeds received on the sterile line were the seeds of the female parent.

[0065] 2. Hybrid seed production technology

[0066] Due to the relatively large amount of seed production of the first-generation hybrid species, open-air cultivation is generally used for seed production.

[0067] 1) Strict isolation of seed production bases: open-air seed production bases generally adopt spatial isolation, that is, other varieties or crops that can be hybridized with broccoli cannot be planted within a radius of...

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Abstract

The invention discloses a breeding method of a broccoli variety with high glucoraphanin content in broccoli florets and seeds. According to the invention, a broccoli double haploid (DH) line obtainedby microspore culture is used as a male parent, a cytoplasmic male sterile line of a high-glucoraphanin-content broccoli variety obtained by multi-generation backcross transformation is used as a female parent, and sexual hybridization is performed to obtain the novel high-glucoraphanin-content broccoli variety. According to the high-glucoraphanin-content broccoli variety obtained through breedingin the invention, the content of glucoraphanin in the broccoli florets is 2.5 times or above the content of glucoraphanin in a common variety (a commercially-available variety with the highest glucoraphanin content), the content of glucoraphanin in the broccoli seeds is 107 micromole / g or above, and the broccoli seeds do not contain alkenylated glucosinolate components.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a breeding method for varieties with high glucoraphanin content in broccoli curds and seeds. Background technique [0002] Broccoli (Brassica oleracea L.var.italica), also known as broccoli and green cauliflower, is a vegetable crop in Brassicaceae Brassica. Scientific research has found that the isothiocyanates produced by the degradation of some glucosinolates in broccoli have beneficial biological activities for the human body, especially those produced by the degradation of glucoraphanin (scientific name: 4-methyl-sulfinylbutyl glucosinolate) The isothiocyanate is sulforaphane (also known as sulforaphane), which can not only reduce the occurrence of various cancers, such as colon cancer, pancreatic cancer, bladder cancer, etc., but also reduce the occurrence of cardiovascular diseases. As of early 2020, the official website of clinical trials in the United...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 王建升顾宏辉虞慧芳赵振卿盛小光沈钰森
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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