Method for preparing glucoraphanin from broccoli

Abstract

The invention discloses a method for preparing glucoraphanin from broccoli, which comprises the following steps: cleaning the raw material, chopping, adding into an extraction tank set host, and sufficiently contacting solid-phase and liquid-phase substances in the extraction tank set in a reverse motion process, thereby extracting effective components from the medicinal material. The medicine slag are forcedly pushed to a slag outlet to be discharged by a discharge conveyer, and a special squeezing machine extrudes the medicine slag to obtain medicinal material tissues from the residual liquor in the medicine slag, thereby reducing the residual liquor content in the medicine slag. The method has the advantages of higher extraction efficiency and more complete extraction. By adopting the macroporous adsorbent resin combined with silica gel column chromatography for purification, the separating effect is better. The method can be used for preparing the glucoraphanin finished product of which the maximum yield is 1% and the content is higher than 95%.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a preparation method of glucoraphanin, in particular to a preparation method of separating and purifying glucoraphanin by using a macroporous adsorption resin coupled with silica gel column chromatography. Background technique: [0002] Cauliflower (scientific name: Brassica oleracea L.var.botrytis L.), also known as cauliflower, cauliflower or cauliflower, is a cruciferous vegetable, a variant of cabbage. [0003] Biennial herb, 60-90 cm high, covered with pink frost. Stem erect, stout, branched. Basal leaves and lower leaves are oblong to elliptic, 2-3.5 cm long, gray-green, rounded at the top, spreading, not convoluted, entire or with fine teeth, sometimes the leaves are decurrent, with several small lobes, And wing-shaped; petiole 2-3 cm long; middle and upper leaves of stem are small and sessile, oblong to lanceolate, clasping. There is a milky white fleshy head at...

AI Technical Summary

Problems solved by technology

The preparation process of glucoraphanin in the above-mentioned patent documents is mainly extracted through steps such as hot water extraction and chromatographic column separation. Although the final glucoraphanin has a high purity, the process requires too much raw material selection and is not easy for industrial production. , itself costs a lot, and the actual application effect is far inferior to that of macroporous adsorption resin coupled with silica gel column chromatography separation and purification method.