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Method for extracting polyphenol from mango skins

A mango peel and extract technology, which is applied in the direction of medical formula, medical preparations containing active ingredients, anti-toxic agents, etc., can solve the problems of few research reports on mango peel polyphenols, achieve good industrial promotion value, increase extraction rate, The effect of high extraction rate

Inactive Publication Date: 2016-06-01
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many studies on plant polyphenols at home and abroad, but there are few research reports on mango peel polyphenols, mainly on the microbial inhibitory activity and antioxidant activity of mango peel crude extract.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for extracting polyphenols from mango peel, comprising the following steps:

[0024] (1) Take the mango skin, wash it, dry it at 30°C, and pass it through a 60-mesh sieve after crushing;

[0025] (2) Using ethanol solution with a volume fraction of 30% as a solvent, use ultrasonic extraction, suction filtration, and dry the extract to obtain the crude extract of mango peel polyphenols; wherein, the ultrasonic extraction conditions are temperature 60°C, and the ratio of solid to liquid is 1:25 , the extraction time is 60min;

[0026] (3) Purification: Add water to the polyphenol crude extract to prepare a solution of 9 mg / ml, and pass through the macroporous resin at a speed of 3.6 BV / h; after the crude extract solution is finished, use water with an amount of 6 BV to wash the macroporous resin; Then elute with 3BV of ethanol at a concentration of 70% v / v; collect the eluate and dry to obtain mango peel polyphenols.

[0027] After calculation, after ultrasonic...

Embodiment 2

[0029] A method for extracting polyphenols from mango peel, comprising the following steps:

[0030] (1) Take the mango peel, wash it, dry it at 40°C, and pass it through a 60-mesh sieve after crushing;

[0031] (2) Using ethanol solution with a volume fraction of 50% as a solvent, use ultrasonic extraction, suction filtration, and dry the extract to obtain the crude extract of mango peel polyphenols; wherein, the ultrasonic extraction conditions are temperature 75°C, and the ratio of solid to liquid is 1:30 , the extraction time is 90min;

[0032] (3) Purification: add water to the polyphenol crude extract to prepare a solution of 12 mg / ml, and pass through the macroporous resin at a rate of 6.5 BV / h; after the crude extract solution is finished, use water with an amount of 7 BV to wash the macroporous resin; Then elute with ethanol with a dosage of 6BV and a concentration of 75% v / v; collect the eluate and dry it to obtain mango peel polyphenols.

[0033] After calculation...

Embodiment 3

[0035] A method for extracting polyphenols from mango peel, comprising the following steps:

[0036] (1) Take the mango skin, wash it, dry it at 35°C, and pass it through a 60-mesh sieve after crushing;

[0037] (2) Using an ethanol solution with a volume fraction of 40% as a solvent, ultrasonic extraction, suction filtration, and drying of the extract to obtain the crude extract of mango peel polyphenols; wherein, the ultrasonic extraction conditions are a temperature of 60-75°C and a solid-liquid ratio of 1 : 25, extraction time 60-90min;

[0038] (3) Purification: add water to the polyphenol crude extract to prepare a solution of 10 mg / ml, and pass through the macroporous resin at a rate of 5 BV / h; after the crude extract solution is finished, use water with an amount greater than 5 BV to wash the macroporous resin; Elute with 5BV of ethanol with a concentration of 70% v / v; collect the eluate and dry to obtain mango peel polyphenols.

[0039] After calculation, after ultr...

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PUM

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Abstract

The invention discloses a method for extracting polyphenol from mango skins, and belongs to the field of plant extraction. The method comprises the following steps: taking the mango skins, washing the mango skins, drying the washed mango skins at 30-40DEG C, crushing the dried mango skins, and sieving the crushed mango skins by a 60 mesh sieve; carrying out ultrasonic extraction with an ethanol solution with the volume fraction of 30-50% as a solvent, carrying out pumping filtration, and drying the obtained extract liquid to obtain crude mango skin polyphenol extract; adding water to the crude polyphenol extract to prepare a solution with the concentration of 9-12mg / ml, and allowing the solution to go through macro-porous resin at a speed of 3.6-6.5BV / h; cleaning the macro-porous resin with water with the amount being greater than 5BV after the crude extract solution goes through the macro-porous resin; eluting with ethanol with the amount of 3-6BV and the concentration of 70-75% (v / v); and collecting the obtained eluate, and drying the collected eluate to obtain mango skin polyphenols. The method is a new method for utilizing the mango skins, changes wastes into valuables, and has the advantages of simple operation and high extraction rate.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting mango peel polyphenols from mango peel. Background technique [0002] Plant polyphenol (Plant polyphenol), also known as plant tannin (Vegetabletannin), is a complex phenolic secondary metabolite in plants. It has a polyphenol structure and mainly exists in the bark, root, leaf, and fruit of plants. The content is second only to cellulose, hemicellulose and lignin. Flavonoids in polyphenols are antioxidants. Medical research shows that oxidative damage is an important cause of many chronic diseases, such as cardiovascular disease, cancer and aging diseases, and the antioxidant function of polyphenols can prevent these chronic diseases. As an antioxidant, polyphenols have a very good effect on the prevention of cardiovascular diseases, which has been proved by experiments by Chinese and foreign scientists for many years. Since the la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/22A61P39/06
Inventor 陆道猛
Owner GUANGXI UNIV
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