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Extracting method for pork liver metal sulfur protein

A metallothionein and extraction method technology, which is applied in the field of metallothionein extraction, can solve the problems of limited donors, high cost, and inability to meet the needs of national economic development, and achieve large breeding volume, low cost, and enhanced tolerance sexual and adaptive effects

Inactive Publication Date: 2005-02-23
蒋浩然
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the existing products on the market are zinc-containing metallothionein extracted from yeast, snail and rabbit liver. Due to limited donors and high cost, they cannot meet the needs of national economic development.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] Describe in detail below in conjunction with specific embodiment:

[0027] ① induction

[0028] Choose healthy suckling pigs about 5-10kg, and use the prepared ZnSO 4 .7H 2 O solution was induced by intraperitoneal injection, once every other day, a total of 5 times, each dose was 10mg / kg, 20mg / kg, 50mg / kg, 100mg / kg, 150mg / kg, and the total was 330mg / kg.

[0029] Using pigs as donors, the amount of MT produced in pig liver after 5 inductions was 8.6mg / g, which was significantly higher than the amount of MT produced by 3 inductions (4.92mg / g), and 7 inductions produced MT The amount (8.81mg / g) and the amount of MT induced 5 times (8.6mg / g) were not significantly different.

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PUM

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Abstract

Metallothionein is extracted from pork liver by. taking porket as supply source, Intraperitoneal injecting with trace element zinc and inducing, Butchering after 5 times inducing, separating and extracting metallothionein which is well stability and high purity, with high output i.e. 2 to 3 times of extracted from rabbits. The pork liver metallothionein induced with zinc has advantages of low cost, good effect, high output, wide source of inducer, without side effect etc. And pig is a easy feeding animal, wide source, and the pork is edible after inducing to extract metallothionein, The products thereof adapted for anti-oxidation and anti irritability for avian and livestock's feedstuff.

Description

technical field [0001] The invention relates to a method for extracting metallothionein, in particular to a method for extracting pig liver metallothionein. Background technique [0002] Metallothionein (MT) has a strong effect of eliminating free radicals, and can be used alone as a nutritional supplement or in combination with glutathione (GSH), vitamin C (VC), vitamin E (VE), etc., which can effectively inhibit Lipid peroxidation damage, cell protection, lower blood viscosity, improve blood circulation and enhance the body's immunity and other biological functions. Metallothionein can also be used as an antioxidant to prevent oxidative deterioration of food or feed. In addition, metallothionein can combine with many metal ions, participate in the regulation of metal metabolism in the body, balance the homeostasis of metal ions in the body, participate in the operation and excretion of metal ions, and play an important role in mineral nutrition. Metallothionein has been ...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K14/47C07K14/825
Inventor 李丽立
Owner 蒋浩然
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