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Nanoparticle preparation, and preparation method and application thereof

A nanoparticle and preparation technology, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, powder delivery, etc., can solve the problems of poor biological safety, insufficient responsiveness, insufficient targeting, etc., and achieve uniform and adjustable size, The effect of short preparation time and mild experimental conditions

Active Publication Date: 2017-01-11
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, nano-assembled drug carriers still have problems such as 1) poor stability in blood; 2) insufficient responsiveness; 3) insufficient targeting; 4) poor biological safety and other issues that need to be resolved urgently.

Method used

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  • Nanoparticle preparation, and preparation method and application thereof
  • Nanoparticle preparation, and preparation method and application thereof
  • Nanoparticle preparation, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Prepare a molar concentration of 2mM fluorenyl moxycarbonyl-L-histidine aqueous solution (pH value is 6) and a molar concentration of 10mM copper chloride solution (pH value is 5); take 1mL copper chloride solution and add it to 9mL fluorene Mix the methoxycarbonyl-L-histidine aqueous solution evenly; adjust the pH value of the mixed solution to 6.5-7.4 to obtain a nanoparticle preparation.

[0064] The particle size distribution of the obtained nanoparticle preparation is shown in Figure 1(A), showing that the particle size is 120nm and the PDI is 0.12; the Zeta potential of the nanoparticle preparation is -22.8eV, indicating that it is negatively charged. The infrared spectrum of the obtained nanoparticle preparation is shown in Figure 1(B), curve 1 is fluorenylmethoxycarbonyl-L-histidine, and curve 2 is the nanoparticle preparation.

Embodiment 2

[0066] Disperse N-benzyloxycarbonyl-L-histidine-L-phenylalanine in water, adjust the pH value of the solution to 9-11, and dissolve it completely, and finally N-benzyloxycarbonyl-L-histidine- The molar concentration of the L-phenylalanine aqueous solution was 10 mM. Prepare a zinc chloride solution with a molar concentration of 1000mM, adjust the pH of the solution to 3-5; take the above 0.45mL zinc chloride solution and add it to 9mL N-benzyloxycarbonyl-L-histidine-L-phenylalanine aqueous solution and mix uniformly; adjust the pH value of the mixed solution to 6.5-7.4 to obtain a nanoparticle preparation.

[0067] The particle size distribution of the obtained nanoparticle preparation is as follows figure 2 , showing that the particle size is 80nm, and the PDI is 0.06; the Zeta potential of the nanoparticle preparation is -24.9eV. The scanning electron microscope photo of the obtained nanoparticle preparation is shown in Figure 3 (A); the transmission electron microscope pi...

Embodiment 3

[0070] Disperse β-naphthylamino-L-phenylalanine-L-glutamic acid in water, adjust the pH value of the solution to 9-10, and dissolve it completely, and finally β-naphthylamino-L-phenylalanine The molarity of the acid-L-glutamic acid aqueous solution was 100 mM. Prepare a zinc nitrate solution with a molar concentration of 10mM, and adjust the pH value of the solution to 3-5; take the above 5mL zinc nitrate solution and add it to 5mL β-naphthylamino-L-phenylalanine-L-glutamic acid aqueous solution, and mix well; Adjust the pH value of the mixed solution to 6.5-7.4 to obtain a nanoparticle preparation. The scanning electron micrograph of the obtained nanoparticle preparation is shown in Fig. 5(A).

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Abstract

The invention relates to the field of controlled release of biomedical materials and drugs, and particularly relates to a nanoparticle preparation, and a preparation method and application thereof. The nanoparticle preparation is prepared from the following components: (1) oligopeptides and / or oligopeptides derivatives; and (2) metal ions. The nanoparticle preparation is uniform and controllable in particle size, high in drug loading ratio, and good in stability in blood, can quickly release drugs via multiple stimulated response of pH / enzyme / glutathione / metallothionein, and is easy in preparation method and easy for large-scale production.

Description

technical field [0001] The invention relates to the fields of biomedical materials and drug controlled release, relates to the application of nanocarriers in the field of biotechnology, and specifically relates to a nanoparticle preparation, its preparation method and application. Background technique [0002] In recent years, the rapid development of nano-assembly technology in the prevention, diagnosis and treatment of major human diseases has attracted extensive attention and research. Compared with "naked" drugs, nano-drug carriers prepared by nano-assembly technology can change the route of administration and the distribution of drugs in the body, and have target tissue selectivity, improve the bioavailability of drugs, and achieve safe and effective targeted drug delivery and treatment. [0003] These favorable therapeutic effects are achieved based on tumor site-specific microenvironmental properties. The human tumor extracellular environment (pH 5.7-6.8) is more ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K45/06A61P35/00
CPCA61K9/143A61K9/146A61K45/06
Inventor 闫学海李永新沈桂芝李淑坤
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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