101 results about "Zinc metallothionein" patented technology

Devices, such as solid supports having metal binding proteins, such as metallothionein proteins, bound thereto are disclosed for removing metals from substrates in need of having such metals removed therefrom. Specifically membranes having metallothionein proteins from the brine shrimp Artemia are disclosed for removing metals from liquid substrates. Associated methods for removing metals from substrates using metallothionein proteins are also disclosed.

This invention provides a method for expressing human metallothionein (MT) by using plant oil. The method comprises: designing and synthesizing human MT gene according to the preference of plant codon, fusing human MT gene with plant oil protein gene, constructing an expression vector with the fused genes, converting recipient plant by using the expression vector to obtain converted plant, and efficiently expressing human MT by using the seed of the converted plant. Activity experiments show that purified recombinant MT has comparable activity with human origin MT. The method utilizes plant bioreactor to produce MT, thus can avoid possible attack by animal pathogen and toxin in E. coli. The method can largely simplify the separation and purification process of human MT, and lower the production cost, this is advantageous for MT industrialization.

The invention discloses a freeze-dried gel cosmetic and preparation method and application thereof. The freeze-dried gel cosmetic of the invention comprises the following components by weight percent: 0.5-3% of chitosan, 1-10% of stabilizing agent, 0.0001-0.001% of coenzyme Q10, 0.00001-0.001% of metallothionein, 0.00001-0.001% of recombinant human angiopoietin-1 and 0.01-0.2% of low molecular weight heparin sodium, the balance of water. The freeze-dried gel cosmetic of the invention can be used for daily skin care and has the beauty effects of whitening, postponing senility and enhancing skin elasticity.

The invention relates to a composition employing a recombinant human metallothionein fermentation product extract as a core active ingredient and different preparation forms of the composition. The composition is characterized by comprising the recombinant human metallothionein fermentation product extract, oligopeptide-1, a freeze drying agent employing tranexamic acid as a main ingredient, a solution and a liquid crystal essence, wherein the amino acid sequence of recombinant human metallothionein is as shown in SEQ ID No.1; and the amino acid sequence of the oligopeptide-1 is as shown in SEQ ID No.1. The essence has the effects of reducing wrinkles, whitening the skin, moisturizing the skin and preventing ultraviolet radiation; microelements of Zn, Fe, Cu and the like can be supplemented at the same time by removing free radicals of the skin to adjust local microcirculation of the skin and to promote skin repairing and healing, thereby achieving the target of whitening the skin. In addition, the metallothionein has a special function of combining heavy metals; and the essence has a particular effect on makeup people with skin injuries of stains and wrinkles caused by cosmetic pigments containing the heavy metals.

The invention discloses cell protective agent containing metallothionein, which aims to solve the problems that blood cells are damaged during blood perfusion, corresponding tissues and organs during growth are damaged after the cells are cultured and augmented in vitro and transplanted and survival rate is low and the like, and can be used for blood perfusion treatment method and cell treatment method including a method for protecting the corresponding tissues and organs after vitro-culturing, augmenting and transplanting of the cells. The cell protective containing metal sulfur protein can be widely used for protecting blood cells of human bodies with diseases such as uremia, critical illness, intoxication, systemic lupus erythematosus (SLE), severe hepatitis and the like during blood perfusion treatment, and protecting corresponding cultured and augmented cells or transplanted and hyperplasia cells of the corresponding tissues and organs in the cell treatment of diseases caused by severe damage or reduction of the cells.

The present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits by modulating expression in a plant of a nucleic acid sequence encoding a yield increasing polypeptide selected from the group consisting of: an AT-hook motif nuclear localized 19 / 20 (AHL19 / 20), a GRP (Growth Regulating Protein) (wherein said GRP polypeptide is a metallothionein 2a (MT2a) polypeptide), an alanine aminotransferase (AAT)-like polypeptide, and an alanine aminotransferase (AAT) polypeptide. The present invention also concerns plants having modulating expression of a nucleic acid sequence encoding a yield increasing polypeptide selected from the group consisting of: an AT-hook motif nuclear localized 19 / 20 (AHL19 / 20), a GRP (Growth Regulating Protein) (wherein said GRP polypeptide is a metallothionein 2a (MT2a) polypeptide), an alanine aminotransferase (AAT)-like polypeptide, and an alanine aminotransferase (AAT) polypeptide which plants have increased yield-related traits relative to control plants. The invention also provides constructs useful in the methods of the invention.

The invention provides construction of metallothionein fusion protein, rapid preparation of an immobilized carrier and application of the metallothionein fusion protein in heavy metal ion removal. A genetic engineering method is used for fusing encoding genes of metallothionein, carbohydrate binding domain and superfolded green fluorescent protein to obtain a fusion gene mt-cbm-sfGFP. An expression vector is constructed from the fusion gene and transformed into an escherichia coli expression host to be expressed. The expressed protein is immobilized onto an insoluble carbohydrate carrier, andwater-insoluble carbohydrate particles bound with the metallothionein are further applied to remove heavy metal ions in traditional Chinese medicine flos lonicerae japonicae water decoction. The results show that lead ions can be removed quickly and effectively without affecting main active ingredients in the flos lonicerae japonicae water decoction. The method has the advantages of low cost, quickness and convenience and visualization, and can be used for the rapid and effective removal of heavy metals in traditional Chinese medicine water decoction, liquid food and waste water.