Industrial extraction and purification method for rabbit liver zinc metallothionein
A zinc metallothionein and rabbit liver technology, which is applied in the extraction and purification of metallothionein, can solve problems such as unsatisfactory results, achieve the effects of poor mechanical strength of colloidal particles, enhance tolerance and adaptability, and reduce losses
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[0019] 1. Induction
[0020] Choose healthy adult cyanine rabbits about 3kg, and use the prepared ZnSO 4 ·7H 2 O solution was induced by subcutaneous injection in the abdomen, injected every other day, a total of 7 inductions, each dose was 10mg / kg, 20mg / kg, 50mg / kg, 100mg / kg, 150mg / kg, 200mg / kg, 250mg / kg; On the fifteenth day, the rabbits were slaughtered and the livers were taken.
[0021] 2. Rough extraction
[0022] Wash the removed liver with normal saline, weigh 6000g, mince the rabbit liver and put it into the extraction tank-1, add 12L of 0.02M (pH 8.6) Tris-HCl buffer, stir to homogenate, and extract For 30 minutes, add 6L of ethanol: chloroform = 1:0.08 denaturing solution, centrifuge at 4°C, 4000 rpm for 30 minutes, discard the precipitate, take the supernatant, obtain supernatant 1, add supernatant 1 to the extraction In the tank-2, the temperature of the extraction tank-2 was raised to 75°C rapidly, kept for 5 minutes, and then quickly lowered to room temperat...
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