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Squamous cell carcinoma SCC-TCT combined detection method

A technology of SCC-TCT and joint detection, which is applied in the field of SCC-TCT joint detection of squamous epithelial cell carcinoma to achieve the effect of improving work efficiency

Inactive Publication Date: 2015-05-13
HANGZHOU TAISHEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The detection of SCC antigen in exfoliated squamous cell carcinoma cells and the method of combined detection with liquid-based cell TCT have not been involved at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Patient Li XX, the examination of the cervix found that the surface of the cervix was smooth or erosive, hard, and easy to bleed when touched.

[0044] Step (1). Pretreatment of exfoliated cells:

[0045] The gynecologist used a special cervical brush for gynecology to obtain exfoliated cells from the patient's cervix, put them into 10-50wt% methanol solution to fix the exfoliated cells, and obtained the pretreated sample solution, which was stored at 0°C;

[0046] Step (2). Centrifugal aggregation of exfoliated cells:

[0047] First add 20μl 1wt﹪saponin to the test tube, then add 5ml of the sample solution obtained in step (1), centrifuge at 2000 rpm for 10 minutes, and discard the supernatant;

[0048] The saponin is used to destroy red blood cells in the sample solution;

[0049] Step (3). The total amount of exfoliated cells is counted:

[0050] Step (2) The exfoliated cell pellet after centrifugation is collected in a test tube, and 2ml of phosphate buffer solutio...

Embodiment 2

[0065] The examination of the cervix of patient Zhang XX found that the surface of the cervix was smooth and hard.

[0066] Step (1). Pretreatment of exfoliated cells:

[0067] The gynecologist used a special gynecological cervical brush to obtain the exfoliated cells from the patient's cervix, put them in 10% wt% ethanol solution to fix the exfoliated cells, and obtained the pretreated sample solution, which was stored at 8°C;

[0068] Step (2). Centrifugal aggregation of exfoliated cells:

[0069] First add 50μl 1wt﹪saponin to the test tube, then add 10ml of the sample solution obtained in step (1), centrifuge at 3000 rpm for 5 minutes, and discard the supernatant;

[0070] The saponin is used to destroy red blood cells in the sample solution;

[0071] Step (3). The total amount of exfoliated cells is counted:

[0072] Step (4) The exfoliated cell pellet after centrifugation is aggregated in a test tube, add 2ml of phosphate buffer solution with a pH value of 7.4 and mix ...

Embodiment 3

[0085] Patient Wang XX, cervical examination found that the surface of the cervix was smooth, hard, and easy to bleed when touched.

[0086] Step (1). Pretreatment of exfoliated cells:

[0087] The gynecologist used a special gynecological cervical brush to obtain the exfoliated cells from the patient's cervix, put them into 10wt% formaldehyde solution to fix the exfoliated cells, and obtained the pretreated sample solution, which was stored at 5°C;

[0088] Step (2). Centrifugal aggregation of exfoliated cells:

[0089] First add 30μl 1wt﹪saponin to the test tube, then add 6ml of the sample solution obtained in step (1), centrifuge at 2500 rpm for 7 minutes, and discard the supernatant;

[0090] The saponin is used to destroy red blood cells in the sample solution;

[0091] Step (3). The total amount of exfoliated cells is counted:

[0092] Step (2) The exfoliated cell pellet after centrifugation is collected in a test tube, and 2ml of phosphate buffer solution with a pH v...

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Abstract

The invention discloses a squamous cell carcinoma SCC-TCT combined detection method. The squamous cell carcinoma SCC-TCT combined detection method comprises the following steps: sampling exfoliated cells at a cervical part, and preprocessing to obtain a sample solution; centrifugating saponin and the sample solution, taking precipitate, adding a phosphate buffer solution, uniformly mixing, and counting the total cell number; centrifugating a counted cell suspension, adding the phosphate buffer solution for freezing and thawing at least once to obtain a sample solution of which an SCC antigen is dissociated and released; performing intracellular SCC antigen enzyme-labeled determination analysis; carrying out judging and screening according to a determination result, screening out a sample higher than a critical value, and preparing the sample solution corresponding to the sample into a uniform slide on a liquid-based cytometer, fixing, performing papanicolaou staining, performing morphological cell analysis by using a microscope, and judging the morphology of a tumor cell. According to the squamous cell carcinoma SCC-TCT combined detection method, by an SCC-TCT combined detection technology, 80-90% of normal samples are screened out, so that the working efficiency of a pathological doctor can be improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for joint detection of human squamous epithelial cancer antigen cells (SCC) and liquid-based cells (TCT), in particular to a human exfoliated cell, that is, exfoliated squamous epithelial cells Cancer antigen (SCC) detection is a method to screen out positive or suspected samples and then conduct liquid-based cell (TCT) pathological cell morphology analysis. Background technique [0002] Cervical cancer is one of the most common malignant tumors of the female reproductive tract, and its incidence rate is the second among female malignant tumors, accounting for about 11% of all tumors. In some developing countries, the incidence of cervical cancer even exceeds the incidence of breast cancer, becoming the first female malignant tumor. [0003] Squamous cell carcinoma antigen (SCC) is a tumor marker with good specificity and the earliest diagnosis of squamous cell carcinoma. It...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N15/10
CPCG01N33/57411
Inventor 秦炜包知言
Owner HANGZHOU TAISHEN BIOTECH
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