Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Chinese tongue squamous epithelial cell carcinoma cell line CTSC-2 and establishment method thereof

A technology of CTSC-2 and squamous epithelium, applied in the field of Chinese tongue squamous cell carcinoma cell line CTSC-2 and its establishment, can solve the problem of high drug resistance of cells

Inactive Publication Date: 2014-04-30
INST OF DONGGUAN SUN YAT SEN UNIV
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some patients received chemotherapy before surgery, so the cells obtained after surgery have high drug resistance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chinese tongue squamous epithelial cell carcinoma cell line CTSC-2 and establishment method thereof
  • Chinese tongue squamous epithelial cell carcinoma cell line CTSC-2 and establishment method thereof
  • Chinese tongue squamous epithelial cell carcinoma cell line CTSC-2 and establishment method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0058] The establishment method of the Chinese tongue cancer cell line CTSC-2 of the present invention will be further described in conjunction with specific examples below.

[0059] 1. Establishment and Characterization of Tongue Squamous Cell Carcinoma Cell Line CTSC-2

[0060] Material Description:

[0061] 1 clinical specimen

[0062] The oral tissues used in this experiment were all surgically excised specimens from Guanghua Stomatological Hospital Affiliated to Sun Yat-sen University.

[0063] 2 cell lines

[0064] Tongue squamous cell carcinoma CAL27 cell line used in this experiment was purchased from ATCC.

[0065] 3 main instruments

[0066]

[0067]

[0068] 4. Main reagents

[0069]

[0070] 5 main solutions and formulations

[0071] 5.1 Cell culture

[0072] 1) 10×PBS Buffer: NaCl80g, KCl2g, NaCl 2 HPO 4 11.55g, KH 2 PO 4 2g, add about 800ml of ultrapure water, fully stir to dissolve with a magnetic stirrer, adjust the pH value to 7.4, set the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The present invention provides a Chinese tongue squamous epithelial cell carcinoma cell line CTSC-2 establishment method so as to provide material basis for researches on characteristics and treatment drugs of human tongue squamous epithelial cell carcinoma. The method comprises the following specific steps that: a tissue mass adherence method is adopted to carry out primary culture of tongue squamous epithelial cell carcinoma cells; subculture is performed when the cell density achieves about 80%; RT-PCR detection is performed on the stably-passaging cells to show that the cells express marker molecules CK8, 18 and 19 of the human tongue squamous epithelial cell carcinoma cells; and an immunoblotting experiment is adopted to detect expression of proteins of the marker molecules CK8 and 18 of the human tongue squamous epithelial cell carcinoma cells by the adherent cells so as to prove successful establishment of the Chinese tongue squamous epithelial cell carcinoma cell line CTSC-2 from the molecular level.

Description

technical field [0001] The present invention relates to the technical field of human tongue squamous cell carcinoma cells, in particular to a Chinese tongue squamous cell carcinoma cell line CTSC-2 and its establishment method. Background technique [0002] 1.1 Incidence and prognosis of tongue squamous cell carcinoma [0003] Tongue cancer is the most common oral squamous cell carcinoma, usually occurring in the anterior two-thirds of the tongue. Tongue cancer has a high incidence in Southeast Asia, with the highest incidence in India, with an annual incidence rate of 9.4 / 1 million. Studies in recent years have shown that the total incidence of tongue cancer worldwide is gradually increasing, and there is a trend of younger age. Due to the histological characteristics of the site where it occurs and the biological characteristics of tongue cancer, tongue cancer restricts tongue movement in patients, resulting in difficulties in swallowing, speaking, and eating. In additi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N5/095C12Q1/02C12R1/91
Inventor 张雁贺姮张海霞汪华
Owner INST OF DONGGUAN SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com