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Human esophageal carcinoma cell line and application thereof

A cancer cell and esophageal cancer technology, applied in the field of cell lines, can solve the problems of low biodiversity and large differences in biological properties of esophageal cancer, and achieve the effect of stable properties and high tumor formation rate

Inactive Publication Date: 2018-01-05
ANHUI PROVINCIAL HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a new human esophageal cancer cell line and its application

Method used

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  • Human esophageal carcinoma cell line and application thereof
  • Human esophageal carcinoma cell line and application thereof
  • Human esophageal carcinoma cell line and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of ESXC-006 cells

[0042] Fresh clinical esophagus cancer resection specimens were obtained from Anhui Provincial Hospital (female, Chinese, Han nationality, 72 years old, esophagus cancer, pathological diagnosis results: esophagus and cardia excision specimens, esophageal squamous cell carcinoma grade I, invading the submucosa, Peri-esophageal lymph nodes 0 / 4, cardia: adenocarcinoma grades I-II, confined to the submucosa, 0 / 11 pericardial lymph nodes, 0 / 5 subcarinal lymph nodes), immediately immersed in pre-cooled sterile HBSS buffer (containing 500 U / ml penicillin G, 500 μg / ml streptomycin sulfate and 1.25 μg / ml amphotericin B). In a biological safety cabinet, the specimens were washed with fresh sterile HBSS buffer, cut into small pieces of 20-50 mg, and inoculated into immunodeficient SCID mice (purchased from Shanghai Slack Laboratory Animal Co., Ltd.) by subcutaneous puncture. Animals were in good health and behaved normally after inoculati...

Embodiment 2

[0048] Example 2 Biological characteristics and application of ESXC-006 cells

[0049] The present invention firstly adopts the low protein PC-1 without serum TM ESXC-006 cells were cultured in culture medium. When the cells were passaged to the 10th passage, the culture medium was replaced with RPMI1640 medium containing fetal bovine serum and insulin to culture ESXC-006 cells, so that they could grow in vitro for a long time and be stably passaged. When the cells were passed on for more than 20 generations, the cell characters were gradually stabilized, and related biology, genetics and tissue source identification were carried out, and the cells had the same stable characters until the 50th generation. Experimental observation and verification showed that ESXC-006 cells grown in vitro had typical epithelioid morphology, lost contact growth inhibition, and showed malignant growth. Genetic studies confirmed that the cells were aneuploid, with severe aberrations in chromosome...

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Abstract

The invention discloses a human esophageal carcinoma cell line and application thereof. The human esophageal carcinoma cell line is preserved in China Center For Type Culture Collection with a preservation number of CCTCC NO:C201661. Under the premise of retaining the main clinical biological features, the human esophageal carcinoma cell line has the characteristics of high tumor formation rate, short incubation period, good homogeneity and the like, enriches the human esophageal carcinoma cell library, provides powerful scientific research material for study of China population genetic background, and also provides a new experimental material for new drug preclinical study of in vivo and in vitro experiments.

Description

technical field [0001] The invention belongs to the field of cell lines, in particular to a human esophageal cancer cell line and its application. Background technique [0002] Esophageal cancer refers to malignant tumors originating from the esophageal epithelium from the hypopharynx to the esophagogastric junction. Esophageal cancer is one of the most common malignant tumors in human beings, but the incidence level varies greatly and has obvious regional characteristics. my country, especially the country with high incidence of esophageal cancer, has the highest morbidity and mortality in the world. According to statistics, more than 200,000 people die of esophageal cancer every year in the world, of which about 150,000 people die from esophageal cancer every year in my country, accounting for three-quarters of the world's total. In my country, more than 95% of esophageal cancers are squamous cell carcinomas, a few are adenocarcinomas originating from esophageal glands o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02A01K67/027A61K49/00C12R1/91
Inventor 徐美青郭明发柳常青戎保林梅新宇田界勇葛晓梅顾莹谢付波李利孙丽丽李富周禾
Owner ANHUI PROVINCIAL HOSPITAL
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