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Superparamagnetic iron oxide labeled human pancreas cancer cell strain as well as labeling method and application thereof

A pancreatic cancer cell, superparamagnetic technology, applied in the direction of tumor/cancer cells, microbial-based methods, biochemical equipment and methods, etc., can solve the problems of high mortality and difficulty in early diagnosis, achieve short tumor formation time, Strong proliferative ability and high tumor formation rate

Inactive Publication Date: 2010-09-01
川北医学院附属医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pancreatic cancer is a malignant tumor that seriously threatens human health. It is difficult to diagnose early and has a high mortality rate. It is urgent to establish effective methods for early diagnosis, treatment and monitoring of treatment effects.

Method used

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  • Superparamagnetic iron oxide labeled human pancreas cancer cell strain as well as labeling method and application thereof
  • Superparamagnetic iron oxide labeled human pancreas cancer cell strain as well as labeling method and application thereof
  • Superparamagnetic iron oxide labeled human pancreas cancer cell strain as well as labeling method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: In vitro magnetic resonance imaging of SPIO-labeled human pancreatic cancer cells

[0037] 1. Materials and equipment

[0038] Human pancreatic cancer PANC-1 cell line: purchased from Sichuan University;

[0039] SPIO: trade name Resovist (SHU555A), with Fe 3 o 4 and Fe 2 o 3 Nano crystal is the magnetic core, and the outside is covered with carboxy dextran, the particle size (including its coating) is 45nm-60nm, purchased from German SCHERING (Schering) company;

[0040] Poly-lysine stock solution with a mass concentration of 0.1%: purchased from Borik Biological Co., Ltd.;

[0041] Newborn bovine serum: purchased from Beijing Yuanheng Shengma Biotechnology Research Institute;

[0042] 1640 culture medium: purchased from GIBICO;

[0043] Pancreatin: purchased from Beijing Yuanheng Shengma Biotechnology Research Institute

[0044] Phosphate buffer saline (PBS): pH 7.4, self-prepared;

[0045] Six-hole plate: purchased from CORNING

[0046] CO 2 Inc...

Embodiment 2

[0058] Example 2: Detection of growth activity of SPIO-labeled human pancreatic cancer cells

[0059] 1. Materials and equipment

[0060] Human pancreatic cancer PANC-1 cell line: purchased from Sichuan University;

[0061] SPIO: trade name Resovist (SHU555A), with Fe 3 o 4 and Fe 2 o 3 Nano crystal is the magnetic core, and the outside is covered with carboxy dextran, the particle size (including its coating) is 45nm-60nm, purchased from German SCHERING (Schering) company;

[0062] Poly-lysine stock solution with a mass concentration of 0.1%: purchased from Borik Biological Co., Ltd.;

[0063] Newborn bovine serum: purchased from Beijing Yuanheng Shengma Biotechnology Research Institute;

[0064] 1640 culture medium: purchased from GIBICO;

[0065] Pancreatin: purchased from Beijing Yuanheng Shengma Biotechnology Research Institute;

[0066] Phosphate buffer saline (PBS): pH 7.4, self-prepared;

[0067] 96-well plate: purchased from CORNING;

[0068] CO 2 Incubator...

Embodiment 3

[0080] Example 3: Labeling of human pancreatic cancer cell lines with SPIO

[0081] 1. Materials and equipment

[0082] Human pancreatic cancer PANC-1 cell line: purchased from Sichuan University;

[0083] SPIO: trade name Resovist (SHU555A), with Fe 3 o 4 and Fe 2 o 3 Nano crystal is the magnetic core, and the outside is covered with carboxy dextran, the particle size (including its coating) is 45nm-60nm, purchased from German SCHERING (Schering) company;

[0084] Poly-lysine stock solution with a mass concentration of 0.1%: purchased from Borik Biological Co., Ltd.;

[0085] Newborn bovine serum: purchased from Beijing Yuanheng Shengma Biotechnology Research Institute;

[0086] 1640 culture medium: purchased from GIBICO;

[0087] Pancreatin: purchased from Beijing Yuanheng Shengma Biotechnology Research Institute;

[0088] Phosphate buffer saline (PBS): pH 7.4, self-prepared;

[0089] CO 2 Incubator: purchased from Healforce company model HF90;

[0090] Vortex osc...

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Abstract

The invention relates to a superparamagnetic iron oxide labeled human pancreas cancer cell strain, wherein superparamagnetic iron oxide is contained in a cell. In a method for labeling the human pancreas cancer cell strain through the superparamagnetic iron oxide, polylysine is adopted as a transfection medium and is incubated together with the cell through a labeled liquid containing superparamagnetic iron oxide-polylysine, and the method comprises the following steps of: 1. preparation of the labeled liquid; 2. cultivation of the human pancreas cancer cell strain; and 3. labeling of the human pancreas cancer cell strain. The superparamagnetic iron oxide labeled human pancreas cancer cell strain can be applied to magnetic resonance imaging and applied to constructing a pancreas cancer animal model.

Description

technical field [0001] The invention belongs to the field of cell labeling, and in particular relates to a human pancreatic cancer cell line labeled with superparamagnetic iron oxide and a labeling method and application thereof. Background technique [0002] As a branch of molecular imaging, cell imaging is developing rapidly. Currently, the main cell markers used in magnetic resonance imaging (MRI) research are: paramagnetic gadolinium chelate, superparamagnetic iron oxide nanoparticles and perfluorocarbon Wait. Commonly used paramagnetic gadolinium ion contrast agents such as Magnevist, this type of contrast agent itself cannot penetrate the cell membrane into the cell, and the use of gadolinium ion contrast agents to label cells requires cationic liposome mediation and requires a long co-incubation time with cells , so less used for cell labeling. perfluorocarbons are 19 F's magnetic resonance signal changes while imaging can avoid 1 H contrast agent interferes with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09A01K67/027C12Q1/02A61K49/06C12R1/91
Inventor 张小明武超颖蒲宇邵阳
Owner 川北医学院附属医院
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