97results about How to "Many binding sites" patented technology

The invention discloses a high-solid content polyamide polyamine epichlorohydrin wet strength agent, as well as a preparation method and an application thereof. The preparation method comprises the following steps: performing condensation on a dibasic acid and a diamine under the action of an acidic catalyst to get a polyamide polyamine intermediate; enabling the polyamide polyamine intermediate, an organic chloride and a cation active monomer or a mixture of the cation active monomer and sodium hydroxide to perform nucleophilic addition reaction to get a high-viscosity cation polymer; and further adding water-soluble hydroxy amine to react and regulating pH with an acid to get the high-solid content polyamide polyamine epichlorohydrin wet strength agent. The obtained wet strength agent has the advantages of high solid content above 40% (in percentage by weight), high charge density, stability in storage, great wet-strengthening performance and few chlorine-containing byproducts, and can be applied in papermaking wet-end chemistry process.

The invention discloses a negative pole of an enzymatic biofuel cell as well as a preparation method and application of the negative pole. The negative pole is prepared by taking carbon paper as an electrode substrate, an enzyme as a catalyst and a carbon nano-tube/cibacron blue composite as an enzyme carrier, wherein the carbon nano-tube/cibacron blue composite is a carbon nano-tube modified by cibacron blue. The negative pole of the enzymatic biofuel cell disclosed by the invention has rich surface active groups which provide rich bonding sites for the load of enzyme, and has favorable conductivity; an enzymatic biofuel cell electrode prepared by the negative pole of the enzymatic biofuel cell can be used for greatly increasing the power density of the enzymatic biofuel cell.

The invention discloses a method for preparation of triazole molecularly imprinted electrochemical sensor based on an electropolymerized OPD/OAP composite membrane. The method includes glassy carbon electrode pretreatment, electropolymerization of OPD/OAP composite membrane, template molecule elution and other steps in order. According to the invention, a triazole substance is taken as the template molecule, OPD and OAP are adopted as the mixed functional monomer, the use of the composite functional monomer improves the selection performance of the binding site in a polymer film, and is conducive to forming a stable host-guest compound from a template molecule and a functional monomer, so that the template molecule can maintain a good rigid structure in a polymer network structure and form a cavity with memory effect. The preparation method is simple and easy to control, and the sensor has the advantages of strong selectivity, good stability, high sensitivity, low cost, and fast response. The invention also provides application of the sensor in adsorption of triazole substances. The sensor prepared by the method provided by the invention is suitable for analysis and detection of residual triazole pesticide in vegetables, fruits and other agricultural products.

The invention discloses a method for preparing sulfydryl modified ferroferric oxide/silicon dioxide magnetic nanoparticles. The method comprises the following steps that 1, magnetic ferroferric oxide particles are prepared with trivalent ferric salt serving as raw materials through a solvothermal method; 2, tetraethoxysilane is adopted as a silicon source, generated silicon dioxide wraps the surfaces of the magnetic ferroferric oxide particles through a sol-gel method, and ferroferric oxide/silicon dioxide magnetic nanoparticles are formed; 3, the ferroferric oxide/silicon dioxide magnetic nanoparticles are placed in a methylbenzene solution and dispersed evenly to obtain a solution A, dimercaptosuccinate is added to a dimethyl sulfoxide solution and dispersed evenly to obtain a solution B, the solution A and the solution B are mixed and stirred and react for a period of time, then solid-liquid separation, cleaning and drying are carried out, and then the sulfydryl modified ferroferric oxide/silicon dioxide magnetic nanoparticles are obtained. The magnetic nanoparticles are good in dispersing performance, achieve good stability and biocompatibility, and has potential application value in the bio-medical field.

The invention discloses a dual-response type virus molecularly imprinted sensor based on magnetism and photons, and a construction method thereof. The dual-response type virus molecularly imprinted sensor based on magnetism and photons takes magnetic particles as an inner core, the surfaces of the magnetic particles are coated with silicon dioxide, a metal organic framework is grafted to the silicon dioxide, the metal organic framework is connected with a photoresponse polymer, and the photoresponse polymer is connected with a target virus template. The dual-response type virus molecular imprinting sensor based on magnetism and photons has a good binding behavior on a template virus, and is fast in binding kinetics, high in adsorption capacity and good in selectivity.

The invention belongs to the technical field of biological detection and discloses a method for detecting pathogens with sandwich structure through SERS. The method comprises the following steps thatincubation is performed on antimicrobial peptide functionalized magnetic nanoparticles and bacteria, magnetic nanoparticles which adsorb the bacteria are separated out under the effect of an externalmagnetic field to obtain a magnetic nanoparticle-bacterium compound, then SERS probe co-incubation is performed on a mercaptophenylboric acid modified graphene oxide-silver-coated gold nano-compound to form a sandwich structure formed by antimicrobial peptide functionalized magnetic nanoparticle-bacterium-SERS probe, the sandwich structure is separated out under the effect of the external magneticfield, and detection and identification are performed on different bacteria through SERS.

The invention relates to the field of SPR sensor chips, and particularly relates to a ractopamine molecularly imprinted SPR sensor chip as well as a preparation method thereof. A ractopamine molecularly imprinted polymer, graphene nanogold, and 1-octyl-3- methylimidazole hexafluorophosphate are taken as chip substrate modifiers for the ractopamine molecularly imprinted SPR sensor chip. The ractopamine molecularly imprinted SPR sensor chip provided by the invention has high-specificity adsorption capability to ractopamine, and has obvious response signal variation to low-concentration ractopamine in samples. The ractopamine molecularly imprinted SPR chip prepared by the invention is simple in steps, is short in analysis time, and has relatively high accuracy and flexibility when being used for analyzing the low-concentration ractopamine residue.

The invention discloses a soil restoration agent for restoring heavy metal polluted soil and a preparation method thereof. The soil restoration agent is prepared from turfy soil, brown coal, porous modified biocarbon, composite bacterium powder, and medical stone. The preparation method comprises following steps: (1) weighing all raw materials according to a formula; (2) adding brown coal and medical stone into a pulverizer, and grinding the brown coal and medical stone; (3) adding all raw materials into a high speed mixing machine, stirring, and evenly mixing; and (4) carrying out wet granulation to convert the mixture into granules, namely the soil restoration agent. The raw materials are easily available, the preparation technology is scientific, and the soil restoration agent can restore soil polluted by various heavy metals, is capable of improving the quality and structure of soil and increasing the nutrients of soil, and creates great social benefits, economic benefits, and environmental benefits.

The invention relates to an anti-shearing reinforcement device and method for a concrete box girder web. In order to solve the problem that in existing engineering technologies, the concrete box girder web has cracking inclined cracks and insufficient anti-shearing bearing capacity, an external vertical prestressing tendon reinforcement device and method are provided. The device comprises groove-shaped steel, L-shaped steel, vertical prestressing tendons, wedge-shaped steel cushion blocks and an anti-corrosion coating at the outer side of the web. The groove-shaped steel is horizontally fixedto the junction of the upper end of the web and a top plate in the girder length direction. The L-shaped steel (6) is horizontally fixed to the lower end of the web in the girder length direction andis parallel to the groove-shaped steel (5). The vertical prestressing tendons are vertically mounted on the groove-shaped steel and the L-shaped steel, and it is ensured that the vertical prestressingtendons are parallel to the plane of the web. According to the anti-shearing reinforcement device and method, the web is connected with the top plate and a bottom plate through finish rolling twistedsteel, the force transmission path is definite, the risk that web concrete is pulled broken is reduced, and meanwhile, midspan deformation is reduced; the problem of insufficient main tensile stressof the web concrete is effectively solved, and the situations of continuous extension of the inclined cracks of the web or new inclined cracks are prevented.

The invention belongs to the field of immunological detection and particularly relates to a method for evaluating the blocking activity of an immune checkpoint molecular blocking antibody by magneticbeads. Magnetic beads with solid-phase anti-tag molecular antibodies and immune checkpoint molecular protein expressing label molecules (label I) subjected to solid-phase on the surfaces of the magnetic beads are used; the magnetic beads can be specifically bound with a ligand of an immune checkpoint molecule with another label molecule (label II), and then a label II specific binding antibody labeled by a biologic substance (fluorescent or visible light) is used for quantitatively detecting the immune checkpoint molecular protein/ligand molecular protein compound on the surface of the magnetic bead. When the blocking antibody of the immune checkpoint molecule, the magnetic beads with the immune checkpoint molecule protein in the solid phase and the ligand protein coexist, the blocking activity of the antibody is inversely proportional to the final luminous intensity of the magnetic beads, so that quantitative analysis on the blocking activity of the antibody is realized through lightintensity detection. The magnetic bead method is simple to operate, quick in reaction, economical and time-saving.

The invention discloses a molecule imprinted fluorescent sensor for detecting viruses. A metal organic framework is used as a carrier material of the sensor, a metal chelator is used as a functional monomer, and a six-membered ring is formed between a template molecule and the functional monomer through metal chelation, so as to prepare the sensor. Specific identification capability of the sensorfor template viruses is improved through deactivation. The metal organic framework has high specific surface area, and can provide more imprinting sites, improving response sensitivity of the sensor.Deactivation lowers non-specific adsorption, so that selectivity of the sensor for the template molecule is improved; metal coordination reduces interference of covalent force to template elution andfixes template molecule more effectively relative to non-covalent force; the fluorescent analysis method has advantages of high flexibility, high selectivity, convenience in operation, and so on. Compared with other analysis methods or sensors, the molecule imprinted fluorescent sensor prepared in the invention has high selectivity for template molecules and high detection flexibility, and has high practical application value and significance in biosensing and virus detection and prevention.

The invention discloses a carrier material of immobilized papain and a preparation method of the carrier material. The method comprises the steps as follows: liquid paraffin and trichloromethane are mixed, then an emulsifier is added, the mixture is uniformly stirred and kept at the temperature of 60-70 DEGC, a mixed solution is added to the mixture with stirring, the mixture is continuously stirred for 20-30 min after addition and cooled to the room temperature, a supernatant is removed through centrifugation, prepared microspheres are washed three times with petroleum ether and then washed with water, and the carrier material of the immobilized papain is prepared. The invention further discloses the immobilized papain from the carrier material and a preparation method of the immobilized papain. The carrier material can be firmly combined with the papain, and the papain cannot fall off easily during usage. Besides, the papain can still keep higher activity after being immobilized on the carrier.

The invention relates to the technical field of catalysts, and discloses a Ni-NiO/C core-shell structure nanomaterial electrocatalyst and a preparation method thereof so as to solve the problem that an existing electrocatalyst is low in catalytic efficiency of catalytic hydrogen evolution; the preparation method comprises the following steps: (1) dissolving nickel chloride hexahydrate in distilled water to form a first solution; (2) adding aminoacetic acid and isopropanol into the first solution to form a second solution; (3) stirring the second solution to form turbid liquid, transferring the turbid liquid into a container, carrying out gradient heating reaction, and cooling to room temperature to obtain light green precipitate; (4) washing the collected light green precipitate cleanly, and performing vacuum drying to obtain a precursor; (5) preliminarily calcining the precursor in inert gas to obtain a Ni/C compound; and (6) carrying out secondary calcination on the Ni/C compound in air. The synthesis method provided by the invention has the characteristics of simple process, low energy consumption, mild conditions, good product morphology and the like, and is suitable for large-scale production and application.