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Method of evaluating skin sensitivity level by using squamous cell carcinoma-related antigen as indication

An evaluation method, squamous epithelial technology, applied in the field of evaluation of skin sensitivity, can solve problems such as unclear

Active Publication Date: 2009-04-08
SHISEIDO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High expression of SCCA-1 and SCCA-2 is known to be caused by UV in diseases such as psoriasis, but how they are involved in skin traits is not clear

Method used

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  • Method of evaluating skin sensitivity level by using squamous cell carcinoma-related antigen as indication
  • Method of evaluating skin sensitivity level by using squamous cell carcinoma-related antigen as indication
  • Method of evaluating skin sensitivity level by using squamous cell carcinoma-related antigen as indication

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Experimental program
Comparison scheme
Effect test

Embodiment

[0031] Materials and methods

[0032] (1) Antibody

[0033] Polyclonal Antibodies Recognizing Both SCCA-1 and 2 Polyclonal antibodies were prepared using SCCA (SCCA-1 and SCCA-2) purified from psoriatic epidermis scales. The scale extract of the psoriasis epidermis (extract solution: 0.1M Tris-HCl (pH8.0), 0.14M NaCl) was centrifuged, and then the supernatant was washed with Sephacryl S-200, DEAE Sepharose, Mono Q, Mono S, Mono P, Superrose 6 was purified and used as an antigen, and rabbits were used as sensitized animals.

[0034] Anti-SCCA-1 monoclonal antibody and anti-SCCA-2 monoclonal antibody were obtained from Santa Cruz Biotechnology, CA, USA.

[0035] (2)ELISA

[0036] The skin stratum corneum sample was collected by a tape peeling method in which a transparent adhesive tape (セロテプ (registered trademark) (NICHIBAN)) was attached to the skin surface and then peeled off. The tape attached to the stratum corneum of the skin was cut off, immersed in an extraction buffe...

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Abstract

It is intended to provide a method of evaluating a skin sensitivity level by using the expression of squamous cell carcinoma related antigen (SCCA) in skin horny layer cells as an indication.

Description

technical field [0001] The present invention provides a method for evaluating the degree of skin sensitivity using Squamous Cell Carcinoma Antigen 1 (hereinafter referred to as "SCCA") of cells as an index. Background technique [0002] In recent years, the number of people who consider their skin to be sensitive skin, that is, highly sensitive skin showing strong reactivity to external stimuli and stress, tends to increase. Especially among young women aged 20 to 30, more than 70% of people answered that their skin is sensitive skin in public opinion surveys. The main causes of skin sensitivity include a decrease in skin barrier function, a decrease in skin irritation threshold, dryness of the skin, inflammatory substances in contact dermatitis, physical and chemical stimulation, stress, physical conditions, seasonal changes, ultraviolet rays , Physiology, etc. In addition, even if the skin is resistant to stress, etc., it may be reported as sensitive skin, and appropriat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N33/6881G01N2333/705G01N33/5091
Inventor 片桐千华仲西城太郎日比野利彦
Owner SHISEIDO CO LTD
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