Squamous cell carcinoma antigen detection reagent

Abstract

The invention provides a squamous cell carcinoma antigen detection reagent. The squamous cell carcinoma antigen detection reagent comprises magnetic microspheres coating squamous cell carcinoma antigen antibodies, squamous cell carcinoma antigen antibodies labeled by alkaline phosphatase, and a chemiluminescent substrate; and the magnetic microspheres are silicon dioxide magnetic composite particles. The squamous cell carcinoma antigen detection reagent provided by the invention has the advantages of simple components and high detection sensitivity, and can realize rapid and accurate detectionin a short time.

Description

technical field [0001] The invention belongs to the technical field of medical detection and relates to a squamous cell carcinoma antigen detection reagent. Background technique [0002] Squamous cell carcinoma antigen (Squamous Cell Careinoma Antigen, SCCA) is a very specific tumor marker that was first used to diagnose cancer. He is a glycoprotein extracted from uterine tissue, which belongs to the serine / cysteine ​​protease inhibitor family. It consists of SCCA1 and SCCA2, which are highly homologous but have completely different biological functions. Both contain 390 amino acids. The molecular weight is about 45kDa. SCCA widely exists in normal tissues of different organs (minimal content) and epithelial cells of malignant lesions; the concentration of SCCA in serum is related to the degree of differentiation of squamous cell carcinoma, and the concentration of SCCA in normal human serum is less than 1.5ng / mL. Elevated SCC can also be seen in a few benign diseases, suc...

AI Technical Summary

Problems solved by technology

However, the ELISA method has large limitations, poor specificity, low sensitivity and low accuracy

Although radioimmunotherapy is considered to be an accurate and reliable method, the radiation effects of radioisotopes, the need for specialized laboratories for waste disposal, the disadvantages of high cost and short half-life have made this method gradually phased out

Latex-enhanced immunoturbidimetry is simple and fast, but has low sensitivity and poor reproducibility

[0004] CN103454417A discloses a preparation method and application of a squamous cell carcinoma antigen biosensor. It prepares a sandwich type electrochemical immunosensor based on nitrogen-doped graphene and Pd-Au / C loaded nanocatalyst, which can Achieve rapid detection of squamous cell carcinoma antigens, but detection accuracy is low

CN104614526A discloses a squamous intracellular carcinoma SCC-TCT combined detection method; the method is to sample the exfoliated cells of the cervix and obtain a sample solution after pretreatment; centrifuge the saponin and the sample solution to take the precipitate, add phosphoric acid After mixing the salt buffer solution, count the total number of cells; centrifuge the counted cell suspension and add phosphate buffer solution for at least one freeze-thaw to obtain the free and released sample solution of the SCC antigen; then perform cell Enzyme standard test and analysis of internal SCC antigen; according to the determination results, the screening is judged, and the specimens higher than the critical value are screened out, and the sample solution corresponding to the specimen is made into a uniform smear on a liquid-based cytometer, fixed, and stained by Papanicolaou staining , microscopic cell morphology analysis, to judge the shape of tumor cells; this method is too complicated

Images