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Chinese people tongue squamous epithelial cell cancer cell line CTSC-1 and establishment method thereof

A technology of CTSC-1 and squamous epithelium, applied in the field of Chinese tongue squamous cell carcinoma cell line CTSC-1 and its establishment, can solve the problem of high drug resistance of cells

Inactive Publication Date: 2014-08-06
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some patients received chemotherapy before surgery, so the cells obtained after surgery have high drug resistance

Method used

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  • Chinese people tongue squamous epithelial cell cancer cell line CTSC-1 and establishment method thereof
  • Chinese people tongue squamous epithelial cell cancer cell line CTSC-1 and establishment method thereof
  • Chinese people tongue squamous epithelial cell cancer cell line CTSC-1 and establishment method thereof

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Embodiment Construction

[0057] The establishment method of the Chinese tongue cancer cell line CTSC-1 of the present invention will be further described in conjunction with specific examples below.

[0058] 1. Establishment and Characterization of Tongue Squamous Cell Carcinoma Cell Line CTSC-1

[0059] Material Description:

[0060] 1 clinical specimen

[0061] The oral tissues used in this experiment were all surgically excised specimens from Guanghua Stomatological Hospital Affiliated to Sun Yat-sen University.

[0062] 2 cell lines

[0063] Tongue squamous cell carcinoma CAL27 cell line used in this experiment was purchased from ATCC

[0064] 3 main instruments

[0065]

[0066]

[0067] 4. Main reagents

[0068]

[0069]

[0070] 5 main solutions and formulations

[0071] 5.1 Cell Culture

[0072] 1) 10×PBS Buffer: NaCl80g, KCl2g, NaCl 2 HPO 4 11.55g, KH 2 PO 4 2g, add about 800ml of ultrapure water, fully stir to dissolve with a magnetic stirrer, adjust the pH value to 7....

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Abstract

The invention provides an establishment method of a Chinese people tongue squamous epithelial cell cancer cell line and provides a material basis for research on characteristics and treatment drugs of people tongue squamous epithelial cell cancers. The establishment method comprises specific steps as follows: primary culture is performed on tongue squamous epithelial cell cancer cells with a tissue explant adherent method; subculture is performed when the cell density reaches about 80%; RT-PCR (reverse transcription-polymerase chain reaction) detection indicates that cells which are stable in subculture express people tongue squamous epithelial cell cancer cell indicative molecules CK8, 18 and 19; and western blot experiment detection indicates that adherent cells express people tongue squamous epithelial cell cancer cell indicative molecule CK8 and 18 proteins, so that the successful establishment of the Chinese people tongue squamous epithelial cell cancer cell line CTSC-1 is proved at the molecular level.

Description

technical field [0001] The present invention relates to the technical field of human tongue squamous cell carcinoma cells, in particular to a Chinese tongue squamous cell carcinoma cell line CTSC-1 and its establishment method. Background technique [0002] 1.1 Incidence and prognosis of tongue squamous cell carcinoma [0003] Tongue cancer is the most common oral squamous cell carcinoma, usually occurring in the anterior two-thirds of the tongue. Tongue cancer has a high incidence in Southeast Asia, with the highest incidence in India, with an annual incidence rate of 9.4 / 1 million. Studies in recent years have shown that the total incidence of tongue cancer worldwide is gradually increasing, and there is a trend of younger age. Due to the histological characteristics of the site where it occurs and the biological characteristics of tongue cancer, tongue cancer restricts tongue movement in patients, resulting in difficulties in swallowing, speaking, and eating. In additi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/095C12Q1/02C12R1/91
Inventor 张雁贺姮张海霞汪华
Owner SUN YAT SEN UNIV
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