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SCC (squamous-cell carcinoma) antigen chemiluminescence immunoassay kit and preparation method thereof

A chemiluminescent immunoassay and detection kit technology, which is applied in the direction of biological testing, material inspection products, etc., can solve the problems of long operation time, narrow linear range, poor accuracy, etc., achieve strong anti-interference ability, improve accuracy, shorten the effect of time

Inactive Publication Date: 2019-01-04
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a squamous cell carcinoma antigen chemiluminescent immunoassay kit and its preparation method in order to solve the problems of long operating time, poor accuracy and narrow linear range in the existing SCC assay kits

Method used

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  • SCC (squamous-cell carcinoma) antigen chemiluminescence immunoassay kit and preparation method thereof
  • SCC (squamous-cell carcinoma) antigen chemiluminescence immunoassay kit and preparation method thereof
  • SCC (squamous-cell carcinoma) antigen chemiluminescence immunoassay kit and preparation method thereof

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preparation example Construction

[0040] The present invention also provides a preparation method of squamous cell carcinoma antigen chemiluminescence immunoassay kit, the preparation process is as follows: figure 1 shown, including the following steps:

[0041] Step 1: Preparation of streptavidin magnetic particle suspension

[0042] After mixing the streptavidin magnetic particle solution and TBST solution, place it on a magnetic separator until the supernatant is free of turbidity, discard the supernatant, keep the magnetic particles, and prepare a solid phase reagent in the buffer after washing , to obtain streptavidin magnetic particle suspension;

[0043] The concentration of the streptavidin magnetic particle solution is preferably 50-100 mg / ml; the volume ratio of the streptavidin magnetic particle solution to the TBST solution is preferably (0.5-1): (5-10); The mixing time is preferably 10-15 minutes, the buffer is 50mM MES, 0.05% Tween-20, 0.05% Proclin300, pH6.5; the concentration of the solid-pha...

Embodiment 1

[0052] Embodiment 1: Preparation of squamous cell carcinoma antigen chemiluminescent immunoassay kit:

[0053] (1) Preparation of streptavidin magnetic particle suspension

[0054] Take 0.72 ml (72 mg) of streptavidin magnetic particle solution with a concentration of 100 mg / ml, add 10 mL of TBST solution and mix well for 10 minutes, then place it on a magnetic separator until the supernatant is free of turbidity, discard the supernatant, Keep the magnetic particles. After repeated washing 3 times, a solid-phase reagent with a magnetic bead concentration of 0.072% was formulated in a buffer solution of 50mM MES, 0.05% Tween, 0.05% Proclin300, pH7.4, and stored at 2-8°C.

[0055] (2) Preparation of acridinium ester-labeled squamous cell carcinoma antigen monoclonal antibody

[0056] Put 500ug of antibody into a centrifuge tube to ensure that the antibody is at the bottom of the centrifuge tube (centrifuge at room temperature for 20s), then add phosphate buffer solution, mix w...

Embodiment 2

[0059] Example 2 Preparation of squamous cell carcinoma antigen chemiluminescent immunoassay kit

[0060] (1) Preparation of streptavidin magnetic particle suspension

[0061] Take 0.72 ml (72 mg) of streptavidin magnetic particle solution with a concentration of 100 mg / ml, add 10 mL of TBST solution and mix well for 12 minutes, place on a magnetic separator until the supernatant is free of turbidity, discard the supernatant, Keep the magnetic particles. After repeated washing 3 times, a solid-phase reagent with a magnetic bead concentration of 0.072% was formulated in a buffer solution of 50mM MES, 0.05% Tween, 0.05% Proclin300, pH7.4, and stored at 2-8°C.

[0062] (2) Preparation of acridinium ester-labeled squamous cell carcinoma antigen monoclonal antibody

[0063] Put 500ug of antibody into a centrifuge tube to ensure that the antibody is at the bottom of the centrifuge tube (centrifuge at room temperature for 30s), then add phosphate buffer solution, mix well, add 5μl ...

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Abstract

The invention provides a SCC (squamous-cell carcinoma) antigen chemiluminescence immunoassay kit and a preparation method thereof, and belongs to the technical field of in-vitro detection. The kit comprises a streptavidin magnetic particle suspension, an SCC antigen monoclonal antibody labelled by a chemiluminescent label and a coupling labelled SCC antigen antibody. The invention further providesa preparation method of the SCC antigen chemiluminescence immunoassay kit. The kit is a fully-automatic measurement sample, and directly gives numerical values, so that manual operation errors are reduced, unattended operation is realized, time required for clinical detection is shortened, detection precision is higher, a closed system is formed by a reagent and an instrument, and system errors are small.

Description

technical field [0001] The invention belongs to the technical field of in vitro detection, and in particular relates to a squamous cell carcinoma antigen chemiluminescent immunoassay kit and a preparation method thereof. Background technique [0002] Squamous cell carcinoma antigen (SCC) is a glycoprotein extracted from uterine tissue, referred to as SCC. SCC is a group of glycoproteins belonging to the serine / cysteine ​​protease inhibitor family. It consists of SCC1 and SCC2, which are highly homologous but have distinct biological functions. They both contain 390 amino acids and have a molecular weight of about 45KDa. SCC widely exists in normal tissues (minimal content) and epithelial cells of malignant lesions. The concentration of SCC in serum is related to the degree of differentiation of squamous cell carcinoma, and the concentration of SCC in normal human serum is <1.5ng / ml. Elevated SCC can also be seen in a few benign diseases, such as pulmonary infection, der...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 王凯孟令敏李磊高威孙成艳
Owner DIRUI MEDICAL TECH CO LTD
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